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Visualization of Uptake of Mineral Elements and the Dynamics of Photosynthates in Arabidopsis by a Newly Developed Real-Time Radioisotope Imaging System (RRIS).

Sugita R, Kobayashi NI, Hirose A, Saito T, Iwata R, Tanoi K, Nakanishi TM - Plant Cell Physiol. (2016)

Bottom Line: In contrast, high accumulation of(28)Mg,(45)Ca and(54)Mn was found in the basal part of the main stem.Based on this time-course analysis, the velocity of ion movement in the main stem was calculated, and found to be fastest for S and K among the ions we tested in this study.These results show that this real-time radioisotope imaging system allows visualization of many nuclides over a long time-course and thus constitutes a powerful tool for the analysis of various physiological phenomena.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan.

No MeSH data available.


Visualization of the downward movement of 14C-labeled metabolites from leaves to roots. (a) Schematic of the photography method. Test plants were covered with a polyethylene bag for supply of 14CO2. (b) Serial images of 14C-labeled metabolite movement taken by macro-RRIS. Scale bar = 10 mm. (c) Micro-RRIS image of 14C-labeled metabolites in the root (root tip, maturation area and lateral root), 14C-labeled metabolite image and distribution profile of 14C-labeled metabolites. Scale bar = 100 µm.
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pcw056-F8: Visualization of the downward movement of 14C-labeled metabolites from leaves to roots. (a) Schematic of the photography method. Test plants were covered with a polyethylene bag for supply of 14CO2. (b) Serial images of 14C-labeled metabolite movement taken by macro-RRIS. Scale bar = 10 mm. (c) Micro-RRIS image of 14C-labeled metabolites in the root (root tip, maturation area and lateral root), 14C-labeled metabolite image and distribution profile of 14C-labeled metabolites. Scale bar = 100 µm.

Mentions: When gas was supplied to the rosette leaves, 14C-labeled photosynthates were found to be transported to the root (Fig. 7). To determine the sink tissues within the roots, the downward movement of 14C-labeled photosynthates was visualized after 14CO2 was supplied only to above-ground parts of 2-week-old seedlings, which are juvenile plants before flowering (Fig. 8a). RRIS images of 14C in roots show the arrival of 14C-labeled photosynthates at the root tip areas involved in developing lateral roots as early as 3 h following 14CO2 supply (Fig. 8b). Thereafter, the accumulation of 14C-labeled photosynthates in lateral root tips increased for 12 h, and, after live imaging, the accumulation of 14C-labeled photosynthates between 200 and 800 µm distal to the main root tip was also confirmed using micro-RRIS (Fig. 8c). The root elongation rate in 2-week-old Arabidopsis plants was 5.1 ± 0.4 (SD) mm during 12 h. Therefore, the root segments captured in Fig. 8c were inferred to be newly developed tissues constructed with the 14C-labeled photosynthate.Fig. 8


Visualization of Uptake of Mineral Elements and the Dynamics of Photosynthates in Arabidopsis by a Newly Developed Real-Time Radioisotope Imaging System (RRIS).

Sugita R, Kobayashi NI, Hirose A, Saito T, Iwata R, Tanoi K, Nakanishi TM - Plant Cell Physiol. (2016)

Visualization of the downward movement of 14C-labeled metabolites from leaves to roots. (a) Schematic of the photography method. Test plants were covered with a polyethylene bag for supply of 14CO2. (b) Serial images of 14C-labeled metabolite movement taken by macro-RRIS. Scale bar = 10 mm. (c) Micro-RRIS image of 14C-labeled metabolites in the root (root tip, maturation area and lateral root), 14C-labeled metabolite image and distribution profile of 14C-labeled metabolites. Scale bar = 100 µm.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4836453&req=5

pcw056-F8: Visualization of the downward movement of 14C-labeled metabolites from leaves to roots. (a) Schematic of the photography method. Test plants were covered with a polyethylene bag for supply of 14CO2. (b) Serial images of 14C-labeled metabolite movement taken by macro-RRIS. Scale bar = 10 mm. (c) Micro-RRIS image of 14C-labeled metabolites in the root (root tip, maturation area and lateral root), 14C-labeled metabolite image and distribution profile of 14C-labeled metabolites. Scale bar = 100 µm.
Mentions: When gas was supplied to the rosette leaves, 14C-labeled photosynthates were found to be transported to the root (Fig. 7). To determine the sink tissues within the roots, the downward movement of 14C-labeled photosynthates was visualized after 14CO2 was supplied only to above-ground parts of 2-week-old seedlings, which are juvenile plants before flowering (Fig. 8a). RRIS images of 14C in roots show the arrival of 14C-labeled photosynthates at the root tip areas involved in developing lateral roots as early as 3 h following 14CO2 supply (Fig. 8b). Thereafter, the accumulation of 14C-labeled photosynthates in lateral root tips increased for 12 h, and, after live imaging, the accumulation of 14C-labeled photosynthates between 200 and 800 µm distal to the main root tip was also confirmed using micro-RRIS (Fig. 8c). The root elongation rate in 2-week-old Arabidopsis plants was 5.1 ± 0.4 (SD) mm during 12 h. Therefore, the root segments captured in Fig. 8c were inferred to be newly developed tissues constructed with the 14C-labeled photosynthate.Fig. 8

Bottom Line: In contrast, high accumulation of(28)Mg,(45)Ca and(54)Mn was found in the basal part of the main stem.Based on this time-course analysis, the velocity of ion movement in the main stem was calculated, and found to be fastest for S and K among the ions we tested in this study.These results show that this real-time radioisotope imaging system allows visualization of many nuclides over a long time-course and thus constitutes a powerful tool for the analysis of various physiological phenomena.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan.

No MeSH data available.