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Visualization of Uptake of Mineral Elements and the Dynamics of Photosynthates in Arabidopsis by a Newly Developed Real-Time Radioisotope Imaging System (RRIS).

Sugita R, Kobayashi NI, Hirose A, Saito T, Iwata R, Tanoi K, Nakanishi TM - Plant Cell Physiol. (2016)

Bottom Line: In contrast, high accumulation of(28)Mg,(45)Ca and(54)Mn was found in the basal part of the main stem.Based on this time-course analysis, the velocity of ion movement in the main stem was calculated, and found to be fastest for S and K among the ions we tested in this study.These results show that this real-time radioisotope imaging system allows visualization of many nuclides over a long time-course and thus constitutes a powerful tool for the analysis of various physiological phenomena.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan.

No MeSH data available.


Related in: MedlinePlus

Imaging of 14C-labeled metabolite distribution pattern in Arabidopsis with three supply processings. The 14C distribution images acquired with an imaging plate after RRIS and sample images are described. 14CO2 supply to rosette, whole shoots and inflorescences was performed by (a) pulse–chase experiments and (b) continuous experiments. Scale bar = 20 mm. The arrow indicates a root.
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pcw056-F7: Imaging of 14C-labeled metabolite distribution pattern in Arabidopsis with three supply processings. The 14C distribution images acquired with an imaging plate after RRIS and sample images are described. 14CO2 supply to rosette, whole shoots and inflorescences was performed by (a) pulse–chase experiments and (b) continuous experiments. Scale bar = 20 mm. The arrow indicates a root.

Mentions: Autoradiographic data obtained by live imaging revealed a 14C signal in roots when 14CO2 was supplied to rosette leaves or whole shoots (Fig. 7). In contrast, no 14C signal was observed in roots and rosette leaves when 14CO2 was supplied to the inflorescences (Fig. 7). These results show that there is little phloem flow leaving the inflorescences and that the rosettes are the primary carbon source for roots. Thus the photosynthates fixed in the inflorescence seem to be transported, metabolized and accumulated only within the inflorescence itself.Fig. 7


Visualization of Uptake of Mineral Elements and the Dynamics of Photosynthates in Arabidopsis by a Newly Developed Real-Time Radioisotope Imaging System (RRIS).

Sugita R, Kobayashi NI, Hirose A, Saito T, Iwata R, Tanoi K, Nakanishi TM - Plant Cell Physiol. (2016)

Imaging of 14C-labeled metabolite distribution pattern in Arabidopsis with three supply processings. The 14C distribution images acquired with an imaging plate after RRIS and sample images are described. 14CO2 supply to rosette, whole shoots and inflorescences was performed by (a) pulse–chase experiments and (b) continuous experiments. Scale bar = 20 mm. The arrow indicates a root.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836453&req=5

pcw056-F7: Imaging of 14C-labeled metabolite distribution pattern in Arabidopsis with three supply processings. The 14C distribution images acquired with an imaging plate after RRIS and sample images are described. 14CO2 supply to rosette, whole shoots and inflorescences was performed by (a) pulse–chase experiments and (b) continuous experiments. Scale bar = 20 mm. The arrow indicates a root.
Mentions: Autoradiographic data obtained by live imaging revealed a 14C signal in roots when 14CO2 was supplied to rosette leaves or whole shoots (Fig. 7). In contrast, no 14C signal was observed in roots and rosette leaves when 14CO2 was supplied to the inflorescences (Fig. 7). These results show that there is little phloem flow leaving the inflorescences and that the rosettes are the primary carbon source for roots. Thus the photosynthates fixed in the inflorescence seem to be transported, metabolized and accumulated only within the inflorescence itself.Fig. 7

Bottom Line: In contrast, high accumulation of(28)Mg,(45)Ca and(54)Mn was found in the basal part of the main stem.Based on this time-course analysis, the velocity of ion movement in the main stem was calculated, and found to be fastest for S and K among the ions we tested in this study.These results show that this real-time radioisotope imaging system allows visualization of many nuclides over a long time-course and thus constitutes a powerful tool for the analysis of various physiological phenomena.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan.

No MeSH data available.


Related in: MedlinePlus