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Visualization of Uptake of Mineral Elements and the Dynamics of Photosynthates in Arabidopsis by a Newly Developed Real-Time Radioisotope Imaging System (RRIS).

Sugita R, Kobayashi NI, Hirose A, Saito T, Iwata R, Tanoi K, Nakanishi TM - Plant Cell Physiol. (2016)

Bottom Line: In contrast, high accumulation of(28)Mg,(45)Ca and(54)Mn was found in the basal part of the main stem.Based on this time-course analysis, the velocity of ion movement in the main stem was calculated, and found to be fastest for S and K among the ions we tested in this study.These results show that this real-time radioisotope imaging system allows visualization of many nuclides over a long time-course and thus constitutes a powerful tool for the analysis of various physiological phenomena.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan.

No MeSH data available.


Time-course analysis of 14C-labeled metabolites. Time course of signal intensity of 14C in ROI: main and lateral stem tip from rosette, whole shoots and inflorescences with (a) 14CO2 pulse–chase experiments and (b) 14CO2 continuous experiments. (c) Time course of signal intensity of 14C in two ROIs set on the cauline leaves (C1 and C2) in the sequential images in (a). (d) Time course of total signal intensity of 14C in three ROIs set on the cauline leaves and lateral stem tip in the sequential images in (a).
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pcw056-F6: Time-course analysis of 14C-labeled metabolites. Time course of signal intensity of 14C in ROI: main and lateral stem tip from rosette, whole shoots and inflorescences with (a) 14CO2 pulse–chase experiments and (b) 14CO2 continuous experiments. (c) Time course of signal intensity of 14C in two ROIs set on the cauline leaves (C1 and C2) in the sequential images in (a). (d) Time course of total signal intensity of 14C in three ROIs set on the cauline leaves and lateral stem tip in the sequential images in (a).

Mentions: Phloem flow direction and the source–sink relationship between organs were further studied by analyzing the amounts of 14C-labeled photosynthates accumulated in the tip areas of main and lateral stems (Fig. 6). When 14CO2 was supplied under both pulsed and continuous conditions to the rosette leaves, the signal intensity at the main stem tip increased more than at the lateral stem tip (Fig. 6). In contrast, the amounts of 14C detected at the tips of main and lateral stems did not differ when 14CO2 was supplied to the whole shoots (Fig. 6). These observations suggested the existence of a source organ other than the rosette leaves supplying photosynthates to the lateral stems. To determine whether the potential source organ was the inflorescence, we supplied 14CO2 in pulses to the inflorescence only, and found that the 14C signal intensity in the lateral stems remained higher than that in the main stems, and the 14C signal intensity in the lateral tips continued to increase for up to 20 h, but not in the main stem tip. One explanation for this is that 14C-labeled photosynthates generated in the main stem are continuously transported towards lateral stems. In addition, the 14C signal in cauline leaves of lateral stems decreased (Fig. 6c), although the total signal intensity of 14C in cauline leaves and lateral stem tips was maintained (Fig. 6d). This observation might indicate that cauline leaves also act as a carbon source for lateral stem tips.Fig. 6


Visualization of Uptake of Mineral Elements and the Dynamics of Photosynthates in Arabidopsis by a Newly Developed Real-Time Radioisotope Imaging System (RRIS).

Sugita R, Kobayashi NI, Hirose A, Saito T, Iwata R, Tanoi K, Nakanishi TM - Plant Cell Physiol. (2016)

Time-course analysis of 14C-labeled metabolites. Time course of signal intensity of 14C in ROI: main and lateral stem tip from rosette, whole shoots and inflorescences with (a) 14CO2 pulse–chase experiments and (b) 14CO2 continuous experiments. (c) Time course of signal intensity of 14C in two ROIs set on the cauline leaves (C1 and C2) in the sequential images in (a). (d) Time course of total signal intensity of 14C in three ROIs set on the cauline leaves and lateral stem tip in the sequential images in (a).
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Related In: Results  -  Collection

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pcw056-F6: Time-course analysis of 14C-labeled metabolites. Time course of signal intensity of 14C in ROI: main and lateral stem tip from rosette, whole shoots and inflorescences with (a) 14CO2 pulse–chase experiments and (b) 14CO2 continuous experiments. (c) Time course of signal intensity of 14C in two ROIs set on the cauline leaves (C1 and C2) in the sequential images in (a). (d) Time course of total signal intensity of 14C in three ROIs set on the cauline leaves and lateral stem tip in the sequential images in (a).
Mentions: Phloem flow direction and the source–sink relationship between organs were further studied by analyzing the amounts of 14C-labeled photosynthates accumulated in the tip areas of main and lateral stems (Fig. 6). When 14CO2 was supplied under both pulsed and continuous conditions to the rosette leaves, the signal intensity at the main stem tip increased more than at the lateral stem tip (Fig. 6). In contrast, the amounts of 14C detected at the tips of main and lateral stems did not differ when 14CO2 was supplied to the whole shoots (Fig. 6). These observations suggested the existence of a source organ other than the rosette leaves supplying photosynthates to the lateral stems. To determine whether the potential source organ was the inflorescence, we supplied 14CO2 in pulses to the inflorescence only, and found that the 14C signal intensity in the lateral stems remained higher than that in the main stems, and the 14C signal intensity in the lateral tips continued to increase for up to 20 h, but not in the main stem tip. One explanation for this is that 14C-labeled photosynthates generated in the main stem are continuously transported towards lateral stems. In addition, the 14C signal in cauline leaves of lateral stems decreased (Fig. 6c), although the total signal intensity of 14C in cauline leaves and lateral stem tips was maintained (Fig. 6d). This observation might indicate that cauline leaves also act as a carbon source for lateral stem tips.Fig. 6

Bottom Line: In contrast, high accumulation of(28)Mg,(45)Ca and(54)Mn was found in the basal part of the main stem.Based on this time-course analysis, the velocity of ion movement in the main stem was calculated, and found to be fastest for S and K among the ions we tested in this study.These results show that this real-time radioisotope imaging system allows visualization of many nuclides over a long time-course and thus constitutes a powerful tool for the analysis of various physiological phenomena.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan.

No MeSH data available.