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Visualization of Uptake of Mineral Elements and the Dynamics of Photosynthates in Arabidopsis by a Newly Developed Real-Time Radioisotope Imaging System (RRIS).

Sugita R, Kobayashi NI, Hirose A, Saito T, Iwata R, Tanoi K, Nakanishi TM - Plant Cell Physiol. (2016)

Bottom Line: In contrast, high accumulation of(28)Mg,(45)Ca and(54)Mn was found in the basal part of the main stem.Based on this time-course analysis, the velocity of ion movement in the main stem was calculated, and found to be fastest for S and K among the ions we tested in this study.These results show that this real-time radioisotope imaging system allows visualization of many nuclides over a long time-course and thus constitutes a powerful tool for the analysis of various physiological phenomena.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan.

No MeSH data available.


Photograph of test plants and images of 14C-labeled metabolite movement. (a) Macro-RRIS images of 14C-labeled metabolite. 14CO2 was supplied to rosette leaves. The detection was set at 15 min. Scale bar = 2 cm. (b) 14C distribution images acquired by the imaging plate after the macro-RRIS experiment.
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pcw056-F4: Photograph of test plants and images of 14C-labeled metabolite movement. (a) Macro-RRIS images of 14C-labeled metabolite. 14CO2 was supplied to rosette leaves. The detection was set at 15 min. Scale bar = 2 cm. (b) 14C distribution images acquired by the imaging plate after the macro-RRIS experiment.

Mentions: To investigate the sink–source relationship between rosette leaves and inflorescences, 14CO2 was supplied to the rosette leaves of 43-day-old Arabidopsis plants, and the upward movement of 14C-labeled photosynthates was visualized by RRIS (Fig. 4a). After live imaging for 24 h, samples were placed on an imaging plate (IP), and a distribution image of 14C throughout the plant was acquired (Fig. 4b). The amount of the 14C-labeled metabolite at the main stem was very low, and hardly any signal appeared in the upper part of the main stem. From this, we inferred that rosette leaves were the source organs supplying photosynthates primarily to the lateral stems, while the phloem flow along the sieve tube connecting the basal shoot and the tip region of the main stem was minimal. This result was surprising given that sink parts such as developing flowers and siliques were present on the main stem. We therefore hypothesized that the rosette leaves are the source organs when the stem is young, but, after flowering, the necessary carbon source in the stem is supplied by photosynthates produced in siliques, stems and cauline leaves. To test this hypothesis, the same experiment was performed using a younger plant at 30 d after germination. Accordingly, the movement of the photosynthates was different from that in the 43-day-old plants (Figs. 4a, 5a). The photosynthate produced in the rosette leaves was preferentially transferred to the main stem tip. The direction of phloem flow from rosette leaves towards each stem changed in the basal shoot region and was influenced by the age of the stems.Fig. 4


Visualization of Uptake of Mineral Elements and the Dynamics of Photosynthates in Arabidopsis by a Newly Developed Real-Time Radioisotope Imaging System (RRIS).

Sugita R, Kobayashi NI, Hirose A, Saito T, Iwata R, Tanoi K, Nakanishi TM - Plant Cell Physiol. (2016)

Photograph of test plants and images of 14C-labeled metabolite movement. (a) Macro-RRIS images of 14C-labeled metabolite. 14CO2 was supplied to rosette leaves. The detection was set at 15 min. Scale bar = 2 cm. (b) 14C distribution images acquired by the imaging plate after the macro-RRIS experiment.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836453&req=5

pcw056-F4: Photograph of test plants and images of 14C-labeled metabolite movement. (a) Macro-RRIS images of 14C-labeled metabolite. 14CO2 was supplied to rosette leaves. The detection was set at 15 min. Scale bar = 2 cm. (b) 14C distribution images acquired by the imaging plate after the macro-RRIS experiment.
Mentions: To investigate the sink–source relationship between rosette leaves and inflorescences, 14CO2 was supplied to the rosette leaves of 43-day-old Arabidopsis plants, and the upward movement of 14C-labeled photosynthates was visualized by RRIS (Fig. 4a). After live imaging for 24 h, samples were placed on an imaging plate (IP), and a distribution image of 14C throughout the plant was acquired (Fig. 4b). The amount of the 14C-labeled metabolite at the main stem was very low, and hardly any signal appeared in the upper part of the main stem. From this, we inferred that rosette leaves were the source organs supplying photosynthates primarily to the lateral stems, while the phloem flow along the sieve tube connecting the basal shoot and the tip region of the main stem was minimal. This result was surprising given that sink parts such as developing flowers and siliques were present on the main stem. We therefore hypothesized that the rosette leaves are the source organs when the stem is young, but, after flowering, the necessary carbon source in the stem is supplied by photosynthates produced in siliques, stems and cauline leaves. To test this hypothesis, the same experiment was performed using a younger plant at 30 d after germination. Accordingly, the movement of the photosynthates was different from that in the 43-day-old plants (Figs. 4a, 5a). The photosynthate produced in the rosette leaves was preferentially transferred to the main stem tip. The direction of phloem flow from rosette leaves towards each stem changed in the basal shoot region and was influenced by the age of the stems.Fig. 4

Bottom Line: In contrast, high accumulation of(28)Mg,(45)Ca and(54)Mn was found in the basal part of the main stem.Based on this time-course analysis, the velocity of ion movement in the main stem was calculated, and found to be fastest for S and K among the ions we tested in this study.These results show that this real-time radioisotope imaging system allows visualization of many nuclides over a long time-course and thus constitutes a powerful tool for the analysis of various physiological phenomena.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan.

No MeSH data available.