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Visualization of Uptake of Mineral Elements and the Dynamics of Photosynthates in Arabidopsis by a Newly Developed Real-Time Radioisotope Imaging System (RRIS).

Sugita R, Kobayashi NI, Hirose A, Saito T, Iwata R, Tanoi K, Nakanishi TM - Plant Cell Physiol. (2016)

Bottom Line: In contrast, high accumulation of(28)Mg,(45)Ca and(54)Mn was found in the basal part of the main stem.Based on this time-course analysis, the velocity of ion movement in the main stem was calculated, and found to be fastest for S and K among the ions we tested in this study.These results show that this real-time radioisotope imaging system allows visualization of many nuclides over a long time-course and thus constitutes a powerful tool for the analysis of various physiological phenomena.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan.

No MeSH data available.


Related in: MedlinePlus

Outline of the real-time radioisotope imaging system (RRIS). (a) The radiation was converted to visual light by a CsI (Tl) scintillator deposited on a FOS. The light was introduced to a high-sensitivity CCD camera to produce the radiation profile image. (b) The camera was equipped with a dark box (60×40×40 cm) in which plants were grown. To superimpose a true image on the RRIS image, the digital camera was installed on the other side of the CCD camera. (c) Schematic of the micro-RRIS. To obtain a higher resolution image, RRIS was combined with a microscope.
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pcw056-F1: Outline of the real-time radioisotope imaging system (RRIS). (a) The radiation was converted to visual light by a CsI (Tl) scintillator deposited on a FOS. The light was introduced to a high-sensitivity CCD camera to produce the radiation profile image. (b) The camera was equipped with a dark box (60×40×40 cm) in which plants were grown. To superimpose a true image on the RRIS image, the digital camera was installed on the other side of the CCD camera. (c) Schematic of the micro-RRIS. To obtain a higher resolution image, RRIS was combined with a microscope.

Mentions: We previously developed a real-time radioisotope imaging system (RRIS) to visualize ion movement in plants (Nakanishi et al. 2009). A schematic illustration of the imaging system is shown in Fig. 1. When a radioisotope is supplied to the plant, it emits radiation. The radiation is converted to visual light by a CsI (Tl) scintillator deposited on a fiber optic plate (FOS) (Hamamatsu Photonics Co.). The light is led to a highly sensitive charge-coupled device (CCD) camera to produce a radiation profile image. To date, two imaging systems, macro-RRIS and micro-RRIS, have been developed. The former can image an area of 20×10 cm, and the latter can visualize radioisotope (109Cd) movement under a microscope at a resolution of at most 500 µm (Kanno et al. 2012). RRIS enables the imaging of various types of radiation including β, γ and X-rays (Sugita et al. 2014). Consequently, live imaging of several essential elements such as phosphorus (32P, 33P) and sulfur (35S) is possible (Nakanishi et al. 2011, Kanno et al. 2012).Fig. 1


Visualization of Uptake of Mineral Elements and the Dynamics of Photosynthates in Arabidopsis by a Newly Developed Real-Time Radioisotope Imaging System (RRIS).

Sugita R, Kobayashi NI, Hirose A, Saito T, Iwata R, Tanoi K, Nakanishi TM - Plant Cell Physiol. (2016)

Outline of the real-time radioisotope imaging system (RRIS). (a) The radiation was converted to visual light by a CsI (Tl) scintillator deposited on a FOS. The light was introduced to a high-sensitivity CCD camera to produce the radiation profile image. (b) The camera was equipped with a dark box (60×40×40 cm) in which plants were grown. To superimpose a true image on the RRIS image, the digital camera was installed on the other side of the CCD camera. (c) Schematic of the micro-RRIS. To obtain a higher resolution image, RRIS was combined with a microscope.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836453&req=5

pcw056-F1: Outline of the real-time radioisotope imaging system (RRIS). (a) The radiation was converted to visual light by a CsI (Tl) scintillator deposited on a FOS. The light was introduced to a high-sensitivity CCD camera to produce the radiation profile image. (b) The camera was equipped with a dark box (60×40×40 cm) in which plants were grown. To superimpose a true image on the RRIS image, the digital camera was installed on the other side of the CCD camera. (c) Schematic of the micro-RRIS. To obtain a higher resolution image, RRIS was combined with a microscope.
Mentions: We previously developed a real-time radioisotope imaging system (RRIS) to visualize ion movement in plants (Nakanishi et al. 2009). A schematic illustration of the imaging system is shown in Fig. 1. When a radioisotope is supplied to the plant, it emits radiation. The radiation is converted to visual light by a CsI (Tl) scintillator deposited on a fiber optic plate (FOS) (Hamamatsu Photonics Co.). The light is led to a highly sensitive charge-coupled device (CCD) camera to produce a radiation profile image. To date, two imaging systems, macro-RRIS and micro-RRIS, have been developed. The former can image an area of 20×10 cm, and the latter can visualize radioisotope (109Cd) movement under a microscope at a resolution of at most 500 µm (Kanno et al. 2012). RRIS enables the imaging of various types of radiation including β, γ and X-rays (Sugita et al. 2014). Consequently, live imaging of several essential elements such as phosphorus (32P, 33P) and sulfur (35S) is possible (Nakanishi et al. 2011, Kanno et al. 2012).Fig. 1

Bottom Line: In contrast, high accumulation of(28)Mg,(45)Ca and(54)Mn was found in the basal part of the main stem.Based on this time-course analysis, the velocity of ion movement in the main stem was calculated, and found to be fastest for S and K among the ions we tested in this study.These results show that this real-time radioisotope imaging system allows visualization of many nuclides over a long time-course and thus constitutes a powerful tool for the analysis of various physiological phenomena.

View Article: PubMed Central - PubMed

Affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo, 113-8657 Japan.

No MeSH data available.


Related in: MedlinePlus