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Functional Analysis of an S-Layer-Associated Fibronectin-Binding Protein in Lactobacillus acidophilus NCFM.

Hymes JP, Johnson BR, Barrangou R, Klaenhammer TR - Appl. Environ. Microbiol. (2016)

Bottom Line: Bacterial surface layers (S-layers) are crystalline arrays of self-assembling proteinaceous subunits called S-layer proteins (Slps) that comprise the outermost layer of the cell envelope.To investigate the functional role of LBA0191 (designated FbpB) in L. acidophilus NCFM, an fbpB-deficient strain was constructed.The L. acidophilus mutant with a deletion off bpB lost the ability to adhere to mucin and fibronectin in vitro Homologues off bpB were identified in five additional putative S-layer-forming species, but no homologues were detected in species outside theL. acidophilus homology group.

View Article: PubMed Central - PubMed

Affiliation: Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, North Carolina, USA.

No MeSH data available.


Related in: MedlinePlus

(A) Scanning electron micrographs (SEM) and transmission electron micrographs (TEM) of parent and ΔfbpB strains. SEM images were taken of cells at logarithmic phase (2,500×), and TEM images were taken of cells at stationary phase (10,000×). Arrows indicate S-layer. (B) S-layer thickness (in nanometers) of parent and mutant strains at logarithmic and stationary phases.
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Figure 5: (A) Scanning electron micrographs (SEM) and transmission electron micrographs (TEM) of parent and ΔfbpB strains. SEM images were taken of cells at logarithmic phase (2,500×), and TEM images were taken of cells at stationary phase (10,000×). Arrows indicate S-layer. (B) S-layer thickness (in nanometers) of parent and mutant strains at logarithmic and stationary phases.

Mentions: Examination of cellular morphology by transmission electron microscopy (TEM) reveals a significant reduction in S-layer thickness in the ΔfbpB mutant (Fig. 5). At logarithmic phase, the S-layer was thicker in the parent strain (mean, 14.25 nm; standard deviation [SD], 2.25 nm) than in the mutant strain (mean, 11.58 nm; SD, 1.34 nm). At stationary phase, the S-layer was thicker in the parent strain (mean, 21.59 nm; SD, 4.87 nm) than in the mutant strain (mean, 15.48 nm; SD, 2.47 nm). These differences were significant (P < 0.0001). No morphological differences between strains were visible in scanning electron micrographs (Fig. 5A).


Functional Analysis of an S-Layer-Associated Fibronectin-Binding Protein in Lactobacillus acidophilus NCFM.

Hymes JP, Johnson BR, Barrangou R, Klaenhammer TR - Appl. Environ. Microbiol. (2016)

(A) Scanning electron micrographs (SEM) and transmission electron micrographs (TEM) of parent and ΔfbpB strains. SEM images were taken of cells at logarithmic phase (2,500×), and TEM images were taken of cells at stationary phase (10,000×). Arrows indicate S-layer. (B) S-layer thickness (in nanometers) of parent and mutant strains at logarithmic and stationary phases.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836419&req=5

Figure 5: (A) Scanning electron micrographs (SEM) and transmission electron micrographs (TEM) of parent and ΔfbpB strains. SEM images were taken of cells at logarithmic phase (2,500×), and TEM images were taken of cells at stationary phase (10,000×). Arrows indicate S-layer. (B) S-layer thickness (in nanometers) of parent and mutant strains at logarithmic and stationary phases.
Mentions: Examination of cellular morphology by transmission electron microscopy (TEM) reveals a significant reduction in S-layer thickness in the ΔfbpB mutant (Fig. 5). At logarithmic phase, the S-layer was thicker in the parent strain (mean, 14.25 nm; standard deviation [SD], 2.25 nm) than in the mutant strain (mean, 11.58 nm; SD, 1.34 nm). At stationary phase, the S-layer was thicker in the parent strain (mean, 21.59 nm; SD, 4.87 nm) than in the mutant strain (mean, 15.48 nm; SD, 2.47 nm). These differences were significant (P < 0.0001). No morphological differences between strains were visible in scanning electron micrographs (Fig. 5A).

Bottom Line: Bacterial surface layers (S-layers) are crystalline arrays of self-assembling proteinaceous subunits called S-layer proteins (Slps) that comprise the outermost layer of the cell envelope.To investigate the functional role of LBA0191 (designated FbpB) in L. acidophilus NCFM, an fbpB-deficient strain was constructed.The L. acidophilus mutant with a deletion off bpB lost the ability to adhere to mucin and fibronectin in vitro Homologues off bpB were identified in five additional putative S-layer-forming species, but no homologues were detected in species outside theL. acidophilus homology group.

View Article: PubMed Central - PubMed

Affiliation: Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, North Carolina, USA.

No MeSH data available.


Related in: MedlinePlus