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Role of chemokine RANTES in the regulation of perivascular inflammation, T-cell accumulation, and vascular dysfunction in hypertension.

Mikolajczyk TP, Nosalski R, Szczepaniak P, Budzyn K, Osmenda G, Skiba D, Sagan A, Wu J, Vinh A, Marvar PJ, Guzik B, Podolec J, Drummond G, Lob HE, Harrison DG, Guzik TJ - FASEB J. (2016)

Bottom Line: IFN-γ ex vivo caused significant endothelial dysfunction, which was reduced by superoxide anion scavenging.E., Harrison, D.G., Guzik, T.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Jagiellonian University, Cracow, Poland British Heart Foundation Centre for Excellence, Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, United Kingdom.

No MeSH data available.


Related in: MedlinePlus

Double-negative CD3+CD4−CD8− T cells are abundantly recruited to vasculature in Ang II–dependent hypertension and contribute to IFN-γ production. A) Effect of Ang II–dependent hypertension on mean CD3+CD4−CD8− T-cell content in isolated pVAT in WT and RANTES−/− mice (n = 5 each; example of staining at left). B) Ang II–dependent changes in IFN-γ-producing CD3+CD4−CD8− T cells in pVAT from WT and RANTES−/− mice (n = 5). Data are expressed as means ± sem.
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Figure 6: Double-negative CD3+CD4−CD8− T cells are abundantly recruited to vasculature in Ang II–dependent hypertension and contribute to IFN-γ production. A) Effect of Ang II–dependent hypertension on mean CD3+CD4−CD8− T-cell content in isolated pVAT in WT and RANTES−/− mice (n = 5 each; example of staining at left). B) Ang II–dependent changes in IFN-γ-producing CD3+CD4−CD8− T cells in pVAT from WT and RANTES−/− mice (n = 5). Data are expressed as means ± sem.

Mentions: Because CCR5 are often expressed in IFN-γ-producing T cells, we examined IFN-γ production by T cells within the pVAT. While CD4+ cells did not produce IFN-γ in the pVAT of either sham- or Ang II–infused mice, a small number of CD8 T cells produced this cytokine at baseline, and this is increased in response to Ang II in WT mice. The Ang II–dependent increase in IFN-γ-producing CD8+ T cells was not observed in RANTES−/− mice (Fig. 5D). mRNA expression of IFN-γ was not increased by Ang II in the pVAT of RANTES−/− mice (Fig. 5E). We have previously demonstrated that CD3+CD4−CD8− double-negative T cells were characteristic for hypertensive vasculature. Recruitment of CD3+CD4−CD8− to pVAT is significantly blunted in RANTES−/− mice (Fig. 6A). Moreover, these cells can also produce significant amounts of IFN-γ in Ang II–infused WT mice but not in RANTES−/− mice (Fig. 6B).


Role of chemokine RANTES in the regulation of perivascular inflammation, T-cell accumulation, and vascular dysfunction in hypertension.

Mikolajczyk TP, Nosalski R, Szczepaniak P, Budzyn K, Osmenda G, Skiba D, Sagan A, Wu J, Vinh A, Marvar PJ, Guzik B, Podolec J, Drummond G, Lob HE, Harrison DG, Guzik TJ - FASEB J. (2016)

Double-negative CD3+CD4−CD8− T cells are abundantly recruited to vasculature in Ang II–dependent hypertension and contribute to IFN-γ production. A) Effect of Ang II–dependent hypertension on mean CD3+CD4−CD8− T-cell content in isolated pVAT in WT and RANTES−/− mice (n = 5 each; example of staining at left). B) Ang II–dependent changes in IFN-γ-producing CD3+CD4−CD8− T cells in pVAT from WT and RANTES−/− mice (n = 5). Data are expressed as means ± sem.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4836375&req=5

Figure 6: Double-negative CD3+CD4−CD8− T cells are abundantly recruited to vasculature in Ang II–dependent hypertension and contribute to IFN-γ production. A) Effect of Ang II–dependent hypertension on mean CD3+CD4−CD8− T-cell content in isolated pVAT in WT and RANTES−/− mice (n = 5 each; example of staining at left). B) Ang II–dependent changes in IFN-γ-producing CD3+CD4−CD8− T cells in pVAT from WT and RANTES−/− mice (n = 5). Data are expressed as means ± sem.
Mentions: Because CCR5 are often expressed in IFN-γ-producing T cells, we examined IFN-γ production by T cells within the pVAT. While CD4+ cells did not produce IFN-γ in the pVAT of either sham- or Ang II–infused mice, a small number of CD8 T cells produced this cytokine at baseline, and this is increased in response to Ang II in WT mice. The Ang II–dependent increase in IFN-γ-producing CD8+ T cells was not observed in RANTES−/− mice (Fig. 5D). mRNA expression of IFN-γ was not increased by Ang II in the pVAT of RANTES−/− mice (Fig. 5E). We have previously demonstrated that CD3+CD4−CD8− double-negative T cells were characteristic for hypertensive vasculature. Recruitment of CD3+CD4−CD8− to pVAT is significantly blunted in RANTES−/− mice (Fig. 6A). Moreover, these cells can also produce significant amounts of IFN-γ in Ang II–infused WT mice but not in RANTES−/− mice (Fig. 6B).

Bottom Line: IFN-γ ex vivo caused significant endothelial dysfunction, which was reduced by superoxide anion scavenging.E., Harrison, D.G., Guzik, T.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Jagiellonian University, Cracow, Poland British Heart Foundation Centre for Excellence, Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, United Kingdom.

No MeSH data available.


Related in: MedlinePlus