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Role of chemokine RANTES in the regulation of perivascular inflammation, T-cell accumulation, and vascular dysfunction in hypertension.

Mikolajczyk TP, Nosalski R, Szczepaniak P, Budzyn K, Osmenda G, Skiba D, Sagan A, Wu J, Vinh A, Marvar PJ, Guzik B, Podolec J, Drummond G, Lob HE, Harrison DG, Guzik TJ - FASEB J. (2016)

Bottom Line: IFN-γ ex vivo caused significant endothelial dysfunction, which was reduced by superoxide anion scavenging.E., Harrison, D.G., Guzik, T.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Jagiellonian University, Cracow, Poland British Heart Foundation Centre for Excellence, Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, United Kingdom.

No MeSH data available.


Related in: MedlinePlus

Role of RANTES in Ang II–dependent hypertension and T-cell perivascular infiltration. A) Examples of flow cytometric determination of effects of Ang II infusion on isolated pVAT (minus aorta) infiltration with total leukocytes (CD45+) and T cells (CD3+) in WT and RANTES−/− mice. B) Effect of Ang II–dependent hypertension on mean total leukocyte (CD45+ cells) and T-cell (CD3+) content in isolated pVAT in WT and RANTES−/− mice (n = 5 each). C) Effect of Ang II–dependent hypertension on mean macrophage infiltration in pVAT (n = 5 each). D) Differences in leukocyte subpopulation composition of pVAT upon Ang II infusion in WT and RANTES−/− mice showing notable reduction of T-cell content (n = 5 each). E) Gating strategy for detection of M2 (CD11c−CD206+) and M1 type AT macrophages (CD11c+CD206−) within F4/80+CD11b+ cells. F) Effect of Ang II–dependent hypertension on mean M1 (left) and M2 (right) macrophage infiltration in pVAT upon Ang II infusion in WT and RANTES−/− mice (n = 5 each).
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Figure 4: Role of RANTES in Ang II–dependent hypertension and T-cell perivascular infiltration. A) Examples of flow cytometric determination of effects of Ang II infusion on isolated pVAT (minus aorta) infiltration with total leukocytes (CD45+) and T cells (CD3+) in WT and RANTES−/− mice. B) Effect of Ang II–dependent hypertension on mean total leukocyte (CD45+ cells) and T-cell (CD3+) content in isolated pVAT in WT and RANTES−/− mice (n = 5 each). C) Effect of Ang II–dependent hypertension on mean macrophage infiltration in pVAT (n = 5 each). D) Differences in leukocyte subpopulation composition of pVAT upon Ang II infusion in WT and RANTES−/− mice showing notable reduction of T-cell content (n = 5 each). E) Gating strategy for detection of M2 (CD11c−CD206+) and M1 type AT macrophages (CD11c+CD206−) within F4/80+CD11b+ cells. F) Effect of Ang II–dependent hypertension on mean M1 (left) and M2 (right) macrophage infiltration in pVAT upon Ang II infusion in WT and RANTES−/− mice (n = 5 each).

Mentions: While the infiltration of total leukocytes in pVAT of RANTES−/− mice was modestly reduced compared to that of WT mice, the infiltration of T cells was 50% less in RANTES−/− mice compared to WT mice (Fig. 4A, B). Interestingly, RANTES deficiency was also associated with decreased pVAT macrophage content, but this effect was less pronounced (Fig. 4C). Within the F4/80+CD11b+ macrophages, both CD11c+CD206− (corresponding mainly to M1 polarization) and CD11c−CD206+ (corresponding to M2 polarization) (Fig. 4E) were significantly increased in the pVAT by Ang II infusion (Fig. 4F). Both of these subpopulations in the pVAT were decreased in hypertensive RANTES−/− mice, although this RANTES-related effect was particularly pronounced in relation to CD11c−CD206+ cells (Fig. 4F).


Role of chemokine RANTES in the regulation of perivascular inflammation, T-cell accumulation, and vascular dysfunction in hypertension.

Mikolajczyk TP, Nosalski R, Szczepaniak P, Budzyn K, Osmenda G, Skiba D, Sagan A, Wu J, Vinh A, Marvar PJ, Guzik B, Podolec J, Drummond G, Lob HE, Harrison DG, Guzik TJ - FASEB J. (2016)

Role of RANTES in Ang II–dependent hypertension and T-cell perivascular infiltration. A) Examples of flow cytometric determination of effects of Ang II infusion on isolated pVAT (minus aorta) infiltration with total leukocytes (CD45+) and T cells (CD3+) in WT and RANTES−/− mice. B) Effect of Ang II–dependent hypertension on mean total leukocyte (CD45+ cells) and T-cell (CD3+) content in isolated pVAT in WT and RANTES−/− mice (n = 5 each). C) Effect of Ang II–dependent hypertension on mean macrophage infiltration in pVAT (n = 5 each). D) Differences in leukocyte subpopulation composition of pVAT upon Ang II infusion in WT and RANTES−/− mice showing notable reduction of T-cell content (n = 5 each). E) Gating strategy for detection of M2 (CD11c−CD206+) and M1 type AT macrophages (CD11c+CD206−) within F4/80+CD11b+ cells. F) Effect of Ang II–dependent hypertension on mean M1 (left) and M2 (right) macrophage infiltration in pVAT upon Ang II infusion in WT and RANTES−/− mice (n = 5 each).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836375&req=5

Figure 4: Role of RANTES in Ang II–dependent hypertension and T-cell perivascular infiltration. A) Examples of flow cytometric determination of effects of Ang II infusion on isolated pVAT (minus aorta) infiltration with total leukocytes (CD45+) and T cells (CD3+) in WT and RANTES−/− mice. B) Effect of Ang II–dependent hypertension on mean total leukocyte (CD45+ cells) and T-cell (CD3+) content in isolated pVAT in WT and RANTES−/− mice (n = 5 each). C) Effect of Ang II–dependent hypertension on mean macrophage infiltration in pVAT (n = 5 each). D) Differences in leukocyte subpopulation composition of pVAT upon Ang II infusion in WT and RANTES−/− mice showing notable reduction of T-cell content (n = 5 each). E) Gating strategy for detection of M2 (CD11c−CD206+) and M1 type AT macrophages (CD11c+CD206−) within F4/80+CD11b+ cells. F) Effect of Ang II–dependent hypertension on mean M1 (left) and M2 (right) macrophage infiltration in pVAT upon Ang II infusion in WT and RANTES−/− mice (n = 5 each).
Mentions: While the infiltration of total leukocytes in pVAT of RANTES−/− mice was modestly reduced compared to that of WT mice, the infiltration of T cells was 50% less in RANTES−/− mice compared to WT mice (Fig. 4A, B). Interestingly, RANTES deficiency was also associated with decreased pVAT macrophage content, but this effect was less pronounced (Fig. 4C). Within the F4/80+CD11b+ macrophages, both CD11c+CD206− (corresponding mainly to M1 polarization) and CD11c−CD206+ (corresponding to M2 polarization) (Fig. 4E) were significantly increased in the pVAT by Ang II infusion (Fig. 4F). Both of these subpopulations in the pVAT were decreased in hypertensive RANTES−/− mice, although this RANTES-related effect was particularly pronounced in relation to CD11c−CD206+ cells (Fig. 4F).

Bottom Line: IFN-γ ex vivo caused significant endothelial dysfunction, which was reduced by superoxide anion scavenging.E., Harrison, D.G., Guzik, T.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Jagiellonian University, Cracow, Poland British Heart Foundation Centre for Excellence, Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, United Kingdom.

No MeSH data available.


Related in: MedlinePlus