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Role of chemokine RANTES in the regulation of perivascular inflammation, T-cell accumulation, and vascular dysfunction in hypertension.

Mikolajczyk TP, Nosalski R, Szczepaniak P, Budzyn K, Osmenda G, Skiba D, Sagan A, Wu J, Vinh A, Marvar PJ, Guzik B, Podolec J, Drummond G, Lob HE, Harrison DG, Guzik TJ - FASEB J. (2016)

Bottom Line: IFN-γ ex vivo caused significant endothelial dysfunction, which was reduced by superoxide anion scavenging.E., Harrison, D.G., Guzik, T.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Jagiellonian University, Cracow, Poland British Heart Foundation Centre for Excellence, Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, United Kingdom.

No MeSH data available.


Related in: MedlinePlus

Role of RANTES in T lymphocyte migration in response to Ang II. Experiments were performed in modified Boyden chamber. A, B) Migration of T cells and their subsets and B cells (as reference) from sham- or Ang II–infused mice toward soluble RANTES (10 ng/ml, n = 9). C, D) Chemotaxis of T cells and CD4+ and CD8+ T cells toward conditioned medium from pVAT from sham- and Ang II–infused mice (n = 6 for each). E) Effects of anti-RANTES neutralizing antibody pretreatment on T-cell chemotaxis toward pVAT conditioned medium from sham- and Ang II–treated mice. Isotype antibody–pretreated medium was treated as 100%. Data are expressed as means ± sem.
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Figure 2: Role of RANTES in T lymphocyte migration in response to Ang II. Experiments were performed in modified Boyden chamber. A, B) Migration of T cells and their subsets and B cells (as reference) from sham- or Ang II–infused mice toward soluble RANTES (10 ng/ml, n = 9). C, D) Chemotaxis of T cells and CD4+ and CD8+ T cells toward conditioned medium from pVAT from sham- and Ang II–infused mice (n = 6 for each). E) Effects of anti-RANTES neutralizing antibody pretreatment on T-cell chemotaxis toward pVAT conditioned medium from sham- and Ang II–treated mice. Isotype antibody–pretreated medium was treated as 100%. Data are expressed as means ± sem.

Mentions: To gain additional insight into the role of RANTES, we performed chemotaxis assays of lymphocytes from sham- and Ang II–treated mice toward soluble RANTES in a Boyden chamber. As is evident in Fig. 2A, T cells, but not B cells, from Ang II–infused mice were more avidly attracted to RANTES than T cells from sham-infused animals. This was true for both CD4 and CD8 cells (Fig. 2B). In further studies, we placed conditioned medium from organoid cultures of pVAT from sham- and Ang II–treated mice in the lower portion of a Boyden chamber and examined migration of T cells from Ang II–treated mice. There was minimal migration of T cells toward the conditioned medium of pVAT from sham-infused mice (Fig. 2C); however, CD4+, CD8+, and double-negative CD3+CD4−CD8− T cells were stimulated to migrate toward the conditioned medium of pVAT from Ang II–treated mice (Fig. 2D and Supplemental Fig. S1B, respectively). Importantly, when conditioned media was preincubated with 0.5 μg/ml anti-RANTES antibody (clone 53405; R&D Systems), T-cell chemotaxis toward pVAT from Ang II–infused mice was significantly reduced (Fig. 2E).


Role of chemokine RANTES in the regulation of perivascular inflammation, T-cell accumulation, and vascular dysfunction in hypertension.

Mikolajczyk TP, Nosalski R, Szczepaniak P, Budzyn K, Osmenda G, Skiba D, Sagan A, Wu J, Vinh A, Marvar PJ, Guzik B, Podolec J, Drummond G, Lob HE, Harrison DG, Guzik TJ - FASEB J. (2016)

Role of RANTES in T lymphocyte migration in response to Ang II. Experiments were performed in modified Boyden chamber. A, B) Migration of T cells and their subsets and B cells (as reference) from sham- or Ang II–infused mice toward soluble RANTES (10 ng/ml, n = 9). C, D) Chemotaxis of T cells and CD4+ and CD8+ T cells toward conditioned medium from pVAT from sham- and Ang II–infused mice (n = 6 for each). E) Effects of anti-RANTES neutralizing antibody pretreatment on T-cell chemotaxis toward pVAT conditioned medium from sham- and Ang II–treated mice. Isotype antibody–pretreated medium was treated as 100%. Data are expressed as means ± sem.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836375&req=5

Figure 2: Role of RANTES in T lymphocyte migration in response to Ang II. Experiments were performed in modified Boyden chamber. A, B) Migration of T cells and their subsets and B cells (as reference) from sham- or Ang II–infused mice toward soluble RANTES (10 ng/ml, n = 9). C, D) Chemotaxis of T cells and CD4+ and CD8+ T cells toward conditioned medium from pVAT from sham- and Ang II–infused mice (n = 6 for each). E) Effects of anti-RANTES neutralizing antibody pretreatment on T-cell chemotaxis toward pVAT conditioned medium from sham- and Ang II–treated mice. Isotype antibody–pretreated medium was treated as 100%. Data are expressed as means ± sem.
Mentions: To gain additional insight into the role of RANTES, we performed chemotaxis assays of lymphocytes from sham- and Ang II–treated mice toward soluble RANTES in a Boyden chamber. As is evident in Fig. 2A, T cells, but not B cells, from Ang II–infused mice were more avidly attracted to RANTES than T cells from sham-infused animals. This was true for both CD4 and CD8 cells (Fig. 2B). In further studies, we placed conditioned medium from organoid cultures of pVAT from sham- and Ang II–treated mice in the lower portion of a Boyden chamber and examined migration of T cells from Ang II–treated mice. There was minimal migration of T cells toward the conditioned medium of pVAT from sham-infused mice (Fig. 2C); however, CD4+, CD8+, and double-negative CD3+CD4−CD8− T cells were stimulated to migrate toward the conditioned medium of pVAT from Ang II–treated mice (Fig. 2D and Supplemental Fig. S1B, respectively). Importantly, when conditioned media was preincubated with 0.5 μg/ml anti-RANTES antibody (clone 53405; R&D Systems), T-cell chemotaxis toward pVAT from Ang II–infused mice was significantly reduced (Fig. 2E).

Bottom Line: IFN-γ ex vivo caused significant endothelial dysfunction, which was reduced by superoxide anion scavenging.E., Harrison, D.G., Guzik, T.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine, Jagiellonian University, Cracow, Poland British Heart Foundation Centre for Excellence, Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, United Kingdom.

No MeSH data available.


Related in: MedlinePlus