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Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium.

Wilson C, Saunter CD, Girkin JM, McCarron JG - FASEB J. (2016)

Bottom Line: D., Girkin, J.M., McCarron, J.Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium.

View Article: PubMed Central - PubMed

Affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom; and.

No MeSH data available.


Related in: MedlinePlus

IP3-mediated endothelial Ca2+ response evokes complex but repeatable spatiotemporally signals. A) Time series fluorescence images of active Ca2+ wave fronts showing the progression of ACh (100 µM; bath applied) Ca2+ waves across the endothelium. In 3 separate applications of ACh in the same artery, approximately reproducible Ca2+ patterns of signal progression occurred. Images are composed of instantaneous Ca2+ activity (green) overlaid on standard deviation images (grayscale) indicative of total Ca2+ activity. Scale bars, 100 µm. B) Automatically extracted and temporally aligned Ca2+ signals from data shown in (A).
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Figure 6: IP3-mediated endothelial Ca2+ response evokes complex but repeatable spatiotemporally signals. A) Time series fluorescence images of active Ca2+ wave fronts showing the progression of ACh (100 µM; bath applied) Ca2+ waves across the endothelium. In 3 separate applications of ACh in the same artery, approximately reproducible Ca2+ patterns of signal progression occurred. Images are composed of instantaneous Ca2+ activity (green) overlaid on standard deviation images (grayscale) indicative of total Ca2+ activity. Scale bars, 100 µm. B) Automatically extracted and temporally aligned Ca2+ signals from data shown in (A).

Mentions: Ca2+ waves arising from the initiator ensemble appeared to preferentially travel along the longitudinal axis of the vessel when initially activated by ACh. However, as the paths of Ca2+ waves from different initiating sites crossed, annihilation occurred on collision and the initial large-scale Ca2+ waves decoupled into complex spatiotemporal patterns of Ca2+ signaling (Supplemental Movie 1). Although Ca2+ signaling appeared disordered, the pattern of initial wave progression was consistent on repeated activations (Fig. 6 and Supplemental Movie 3). When the artery was activated consecutively, the average amplitude of the Ca2+ signal in all cells was 0.99 ± 0.04% (n = 7) of control. The reproducible nature of the disordered wave appears to suggest a signal may be encoded in the pattern.


Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium.

Wilson C, Saunter CD, Girkin JM, McCarron JG - FASEB J. (2016)

IP3-mediated endothelial Ca2+ response evokes complex but repeatable spatiotemporally signals. A) Time series fluorescence images of active Ca2+ wave fronts showing the progression of ACh (100 µM; bath applied) Ca2+ waves across the endothelium. In 3 separate applications of ACh in the same artery, approximately reproducible Ca2+ patterns of signal progression occurred. Images are composed of instantaneous Ca2+ activity (green) overlaid on standard deviation images (grayscale) indicative of total Ca2+ activity. Scale bars, 100 µm. B) Automatically extracted and temporally aligned Ca2+ signals from data shown in (A).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836367&req=5

Figure 6: IP3-mediated endothelial Ca2+ response evokes complex but repeatable spatiotemporally signals. A) Time series fluorescence images of active Ca2+ wave fronts showing the progression of ACh (100 µM; bath applied) Ca2+ waves across the endothelium. In 3 separate applications of ACh in the same artery, approximately reproducible Ca2+ patterns of signal progression occurred. Images are composed of instantaneous Ca2+ activity (green) overlaid on standard deviation images (grayscale) indicative of total Ca2+ activity. Scale bars, 100 µm. B) Automatically extracted and temporally aligned Ca2+ signals from data shown in (A).
Mentions: Ca2+ waves arising from the initiator ensemble appeared to preferentially travel along the longitudinal axis of the vessel when initially activated by ACh. However, as the paths of Ca2+ waves from different initiating sites crossed, annihilation occurred on collision and the initial large-scale Ca2+ waves decoupled into complex spatiotemporal patterns of Ca2+ signaling (Supplemental Movie 1). Although Ca2+ signaling appeared disordered, the pattern of initial wave progression was consistent on repeated activations (Fig. 6 and Supplemental Movie 3). When the artery was activated consecutively, the average amplitude of the Ca2+ signal in all cells was 0.99 ± 0.04% (n = 7) of control. The reproducible nature of the disordered wave appears to suggest a signal may be encoded in the pattern.

Bottom Line: D., Girkin, J.M., McCarron, J.Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium.

View Article: PubMed Central - PubMed

Affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom; and.

No MeSH data available.


Related in: MedlinePlus