Limits...
Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium.

Wilson C, Saunter CD, Girkin JM, McCarron JG - FASEB J. (2016)

Bottom Line: D., Girkin, J.M., McCarron, J.Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium.

View Article: PubMed Central - PubMed

Affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom; and.

No MeSH data available.


Related in: MedlinePlus

Intraluminal endothelial imaging. A) Simplified schematic diagram of the endothelial microendoscopy utilizing GRIN. B) Schematic illustrating the optical excitation and emission paths through the side-viewing GRIN microprobe. The GRIN reconjugates the image plane of a conventional microscope through the length of the cylinder (green lines). The collimated input excitation light is recollimated at the output of the GRIN (blue lines). C) An image of the distal end of the GRIN microprobe with the microprism attached. The GRIN is protected inside a stainless steel sheath. D) A 3-dimensional rendering of the system on an inverted microscope showing the custom vessel bath, position of the GRIN microprobe assembly, the objective lens, XYZ translation stage for positioning the artery, and excitation coupling and emission pathway. The laser and camera are outside the image. E) Fluorescence image of the endothelium visualized using the GRIN imaging system. Scale bar, 100 μm. F) Individual baseline corrected Ca2+ signals of corresponding ROIs (E) showing spontaneous Ca2+ transients.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4836367&req=5

Figure 1: Intraluminal endothelial imaging. A) Simplified schematic diagram of the endothelial microendoscopy utilizing GRIN. B) Schematic illustrating the optical excitation and emission paths through the side-viewing GRIN microprobe. The GRIN reconjugates the image plane of a conventional microscope through the length of the cylinder (green lines). The collimated input excitation light is recollimated at the output of the GRIN (blue lines). C) An image of the distal end of the GRIN microprobe with the microprism attached. The GRIN is protected inside a stainless steel sheath. D) A 3-dimensional rendering of the system on an inverted microscope showing the custom vessel bath, position of the GRIN microprobe assembly, the objective lens, XYZ translation stage for positioning the artery, and excitation coupling and emission pathway. The laser and camera are outside the image. E) Fluorescence image of the endothelium visualized using the GRIN imaging system. Scale bar, 100 μm. F) Individual baseline corrected Ca2+ signals of corresponding ROIs (E) showing spontaneous Ca2+ transients.

Mentions: Carotid arteries were obtained from male Sprague-Dawley rats [10–12 wk old; 250–350 gm; killed by overdose of pentobarbital sodium; Schedule 1 procedure; Animal (Scientific Procedures) Act 1986, United Kingdom]. The arteries were mounted onto stainless steel (22 gauge) cannulae in a custom vessel bath, and the endothelium was selectively loaded with a fluorescent Ca2+ indicator by perfusing the lumen with a Ca2+ indicator loading solution for 30 min at 37°C. After loading, arteries were removed from one of the cannulae and remounted on a custom microendoscope fluorescence imaging system to visualize the endothelium and maintain the arteries at physiologic pressures (Fig. 1).


Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium.

Wilson C, Saunter CD, Girkin JM, McCarron JG - FASEB J. (2016)

Intraluminal endothelial imaging. A) Simplified schematic diagram of the endothelial microendoscopy utilizing GRIN. B) Schematic illustrating the optical excitation and emission paths through the side-viewing GRIN microprobe. The GRIN reconjugates the image plane of a conventional microscope through the length of the cylinder (green lines). The collimated input excitation light is recollimated at the output of the GRIN (blue lines). C) An image of the distal end of the GRIN microprobe with the microprism attached. The GRIN is protected inside a stainless steel sheath. D) A 3-dimensional rendering of the system on an inverted microscope showing the custom vessel bath, position of the GRIN microprobe assembly, the objective lens, XYZ translation stage for positioning the artery, and excitation coupling and emission pathway. The laser and camera are outside the image. E) Fluorescence image of the endothelium visualized using the GRIN imaging system. Scale bar, 100 μm. F) Individual baseline corrected Ca2+ signals of corresponding ROIs (E) showing spontaneous Ca2+ transients.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836367&req=5

Figure 1: Intraluminal endothelial imaging. A) Simplified schematic diagram of the endothelial microendoscopy utilizing GRIN. B) Schematic illustrating the optical excitation and emission paths through the side-viewing GRIN microprobe. The GRIN reconjugates the image plane of a conventional microscope through the length of the cylinder (green lines). The collimated input excitation light is recollimated at the output of the GRIN (blue lines). C) An image of the distal end of the GRIN microprobe with the microprism attached. The GRIN is protected inside a stainless steel sheath. D) A 3-dimensional rendering of the system on an inverted microscope showing the custom vessel bath, position of the GRIN microprobe assembly, the objective lens, XYZ translation stage for positioning the artery, and excitation coupling and emission pathway. The laser and camera are outside the image. E) Fluorescence image of the endothelium visualized using the GRIN imaging system. Scale bar, 100 μm. F) Individual baseline corrected Ca2+ signals of corresponding ROIs (E) showing spontaneous Ca2+ transients.
Mentions: Carotid arteries were obtained from male Sprague-Dawley rats [10–12 wk old; 250–350 gm; killed by overdose of pentobarbital sodium; Schedule 1 procedure; Animal (Scientific Procedures) Act 1986, United Kingdom]. The arteries were mounted onto stainless steel (22 gauge) cannulae in a custom vessel bath, and the endothelium was selectively loaded with a fluorescent Ca2+ indicator by perfusing the lumen with a Ca2+ indicator loading solution for 30 min at 37°C. After loading, arteries were removed from one of the cannulae and remounted on a custom microendoscope fluorescence imaging system to visualize the endothelium and maintain the arteries at physiologic pressures (Fig. 1).

Bottom Line: D., Girkin, J.M., McCarron, J.Clusters of specialized detector cells provide sensitive and high fidelity receptor signaling in the intact endothelium.

View Article: PubMed Central - PubMed

Affiliation: Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom; and.

No MeSH data available.


Related in: MedlinePlus