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Increasing Clinical Severity during a Dengue Virus Type 3 Cuban Epidemic: Deep Sequencing of Evolving Viral Populations.

Rodriguez-Roche R, Blanc H, Bordería AV, Díaz G, Henningsson R, Gonzalez D, Santana E, Alvarez M, Castro O, Fontes M, Vignuzzi M, Guzman MG - J. Virol. (2016)

Bottom Line: In conclusion, greater variability was detected during the epidemic's progression in terms of significant minority variants, particularly in the nonstructural genes.We concluded that greater variability in significant minor populations occurred as the epidemic progressed, particularly in the nonstructural genes, with higher variability observed in secondary infection cases.Remarkably, for the first time significant intrahost genetic variation was demonstrated within the same patient during the course of secondary infection with DENV-1/DENV-3, including changes in structural proteins.

View Article: PubMed Central - PubMed

Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba rosmari@ipk.sld.cu.

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Related in: MedlinePlus

Multidimensional scaling using root mean square deviation (RMSD) values calculated using significant minority variants (>1%) for data set a (red dots) and b (blue dots). Numbers represent the 20 studied samples ordered by collection time, as indicated in Table 1. Samples 4 and 5 that correspond to a different lineage were excluded.
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Figure 5: Multidimensional scaling using root mean square deviation (RMSD) values calculated using significant minority variants (>1%) for data set a (red dots) and b (blue dots). Numbers represent the 20 studied samples ordered by collection time, as indicated in Table 1. Samples 4 and 5 that correspond to a different lineage were excluded.

Mentions: Intersample cluster analyses using RMSD values based on MDS showed greater variability with the epidemic's progression. Samples with similar characteristics were reflected in the plot by their close spatial proximity to each other. Isolates collected at the end of the epidemic were located on the periphery of the plotting area, indicating higher variability (Fig. 5). Dendrograms using unique significant minority variants (>0.1%, >0.5%, and >1%) showed similar results; isolates collected at the very beginning were closely related and had less genetic variability than late isolates, based on RMSD values (Fig. 6).


Increasing Clinical Severity during a Dengue Virus Type 3 Cuban Epidemic: Deep Sequencing of Evolving Viral Populations.

Rodriguez-Roche R, Blanc H, Bordería AV, Díaz G, Henningsson R, Gonzalez D, Santana E, Alvarez M, Castro O, Fontes M, Vignuzzi M, Guzman MG - J. Virol. (2016)

Multidimensional scaling using root mean square deviation (RMSD) values calculated using significant minority variants (>1%) for data set a (red dots) and b (blue dots). Numbers represent the 20 studied samples ordered by collection time, as indicated in Table 1. Samples 4 and 5 that correspond to a different lineage were excluded.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836355&req=5

Figure 5: Multidimensional scaling using root mean square deviation (RMSD) values calculated using significant minority variants (>1%) for data set a (red dots) and b (blue dots). Numbers represent the 20 studied samples ordered by collection time, as indicated in Table 1. Samples 4 and 5 that correspond to a different lineage were excluded.
Mentions: Intersample cluster analyses using RMSD values based on MDS showed greater variability with the epidemic's progression. Samples with similar characteristics were reflected in the plot by their close spatial proximity to each other. Isolates collected at the end of the epidemic were located on the periphery of the plotting area, indicating higher variability (Fig. 5). Dendrograms using unique significant minority variants (>0.1%, >0.5%, and >1%) showed similar results; isolates collected at the very beginning were closely related and had less genetic variability than late isolates, based on RMSD values (Fig. 6).

Bottom Line: In conclusion, greater variability was detected during the epidemic's progression in terms of significant minority variants, particularly in the nonstructural genes.We concluded that greater variability in significant minor populations occurred as the epidemic progressed, particularly in the nonstructural genes, with higher variability observed in secondary infection cases.Remarkably, for the first time significant intrahost genetic variation was demonstrated within the same patient during the course of secondary infection with DENV-1/DENV-3, including changes in structural proteins.

View Article: PubMed Central - PubMed

Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba rosmari@ipk.sld.cu.

Show MeSH
Related in: MedlinePlus