Increasing Clinical Severity during a Dengue Virus Type 3 Cuban Epidemic: Deep Sequencing of Evolving Viral Populations.
Bottom Line: In conclusion, greater variability was detected during the epidemic's progression in terms of significant minority variants, particularly in the nonstructural genes.We concluded that greater variability in significant minor populations occurred as the epidemic progressed, particularly in the nonstructural genes, with higher variability observed in secondary infection cases.Remarkably, for the first time significant intrahost genetic variation was demonstrated within the same patient during the course of secondary infection with DENV-1/DENV-3, including changes in structural proteins.
Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba email@example.com.Show MeSH
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Mentions: Intersample cluster analyses using RMSD values based on MDS showed greater variability with the epidemic's progression. Samples with similar characteristics were reflected in the plot by their close spatial proximity to each other. Isolates collected at the end of the epidemic were located on the periphery of the plotting area, indicating higher variability (Fig. 5). Dendrograms using unique significant minority variants (>0.1%, >0.5%, and >1%) showed similar results; isolates collected at the very beginning were closely related and had less genetic variability than late isolates, based on RMSD values (Fig. 6).
Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba firstname.lastname@example.org.