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Increasing Clinical Severity during a Dengue Virus Type 3 Cuban Epidemic: Deep Sequencing of Evolving Viral Populations.

Rodriguez-Roche R, Blanc H, Bordería AV, Díaz G, Henningsson R, Gonzalez D, Santana E, Alvarez M, Castro O, Fontes M, Vignuzzi M, Guzman MG - J. Virol. (2016)

Bottom Line: In conclusion, greater variability was detected during the epidemic's progression in terms of significant minority variants, particularly in the nonstructural genes.We concluded that greater variability in significant minor populations occurred as the epidemic progressed, particularly in the nonstructural genes, with higher variability observed in secondary infection cases.Remarkably, for the first time significant intrahost genetic variation was demonstrated within the same patient during the course of secondary infection with DENV-1/DENV-3, including changes in structural proteins.

View Article: PubMed Central - PubMed

Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba rosmari@ipk.sld.cu.

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Number of positions with unique significant minority variants common for sets a and b (>1%), taking as a reference the first isolate of the DENV-3 Cuban epidemic of 2001 to 2002. Samples are grouped according to type of infection in primary and secondary infections by date of sample collection (Table 1).
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Figure 4: Number of positions with unique significant minority variants common for sets a and b (>1%), taking as a reference the first isolate of the DENV-3 Cuban epidemic of 2001 to 2002. Samples are grouped according to type of infection in primary and secondary infections by date of sample collection (Table 1).

Mentions: However, different results were obtained when higher-frequency minority variants (>1%) were analyzed (Fig. 4). Still greater variability was observed in the nonstructural genes than in the structural genes, but it was noteworthy that patients with secondary infections showed greater variability than patients with primary infections. In addition, patients with secondary infections presented minority variants in the structural genes (PrM and E), some of which were nonsynonymous. In contrast, patients suffering primary infections had only mostly synonymous minority variants (>1%) in nonstructural genes (Tables 4 and 5).


Increasing Clinical Severity during a Dengue Virus Type 3 Cuban Epidemic: Deep Sequencing of Evolving Viral Populations.

Rodriguez-Roche R, Blanc H, Bordería AV, Díaz G, Henningsson R, Gonzalez D, Santana E, Alvarez M, Castro O, Fontes M, Vignuzzi M, Guzman MG - J. Virol. (2016)

Number of positions with unique significant minority variants common for sets a and b (>1%), taking as a reference the first isolate of the DENV-3 Cuban epidemic of 2001 to 2002. Samples are grouped according to type of infection in primary and secondary infections by date of sample collection (Table 1).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836355&req=5

Figure 4: Number of positions with unique significant minority variants common for sets a and b (>1%), taking as a reference the first isolate of the DENV-3 Cuban epidemic of 2001 to 2002. Samples are grouped according to type of infection in primary and secondary infections by date of sample collection (Table 1).
Mentions: However, different results were obtained when higher-frequency minority variants (>1%) were analyzed (Fig. 4). Still greater variability was observed in the nonstructural genes than in the structural genes, but it was noteworthy that patients with secondary infections showed greater variability than patients with primary infections. In addition, patients with secondary infections presented minority variants in the structural genes (PrM and E), some of which were nonsynonymous. In contrast, patients suffering primary infections had only mostly synonymous minority variants (>1%) in nonstructural genes (Tables 4 and 5).

Bottom Line: In conclusion, greater variability was detected during the epidemic's progression in terms of significant minority variants, particularly in the nonstructural genes.We concluded that greater variability in significant minor populations occurred as the epidemic progressed, particularly in the nonstructural genes, with higher variability observed in secondary infection cases.Remarkably, for the first time significant intrahost genetic variation was demonstrated within the same patient during the course of secondary infection with DENV-1/DENV-3, including changes in structural proteins.

View Article: PubMed Central - PubMed

Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba rosmari@ipk.sld.cu.

Show MeSH
Related in: MedlinePlus