Increasing Clinical Severity during a Dengue Virus Type 3 Cuban Epidemic: Deep Sequencing of Evolving Viral Populations.
Bottom Line: In conclusion, greater variability was detected during the epidemic's progression in terms of significant minority variants, particularly in the nonstructural genes.We concluded that greater variability in significant minor populations occurred as the epidemic progressed, particularly in the nonstructural genes, with higher variability observed in secondary infection cases.Remarkably, for the first time significant intrahost genetic variation was demonstrated within the same patient during the course of secondary infection with DENV-1/DENV-3, including changes in structural proteins.
Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba firstname.lastname@example.org.Show MeSH
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Mentions: However, different results were obtained when higher-frequency minority variants (>1%) were analyzed (Fig. 4). Still greater variability was observed in the nonstructural genes than in the structural genes, but it was noteworthy that patients with secondary infections showed greater variability than patients with primary infections. In addition, patients with secondary infections presented minority variants in the structural genes (PrM and E), some of which were nonsynonymous. In contrast, patients suffering primary infections had only mostly synonymous minority variants (>1%) in nonstructural genes (Tables 4 and 5).
Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba email@example.com.