Increasing Clinical Severity during a Dengue Virus Type 3 Cuban Epidemic: Deep Sequencing of Evolving Viral Populations.
Bottom Line: In conclusion, greater variability was detected during the epidemic's progression in terms of significant minority variants, particularly in the nonstructural genes.We concluded that greater variability in significant minor populations occurred as the epidemic progressed, particularly in the nonstructural genes, with higher variability observed in secondary infection cases.Remarkably, for the first time significant intrahost genetic variation was demonstrated within the same patient during the course of secondary infection with DENV-1/DENV-3, including changes in structural proteins.
Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba email@example.com.Show MeSH
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Mentions: Greater variability was observed in the nonstructural genes than in the structural genes in terms of significant minority variants, involving mainly NS5, NS3 and NS4B genes, and particularly toward the end of the epidemic. Across the genome, the number of positions with significant minority variants (>0.1%) ranged from 12 to as high as 48 in both primary and secondary infections without significant differences between these groups (Fig. 3).
Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba firstname.lastname@example.org.