Increasing Clinical Severity during a Dengue Virus Type 3 Cuban Epidemic: Deep Sequencing of Evolving Viral Populations.
Bottom Line: In conclusion, greater variability was detected during the epidemic's progression in terms of significant minority variants, particularly in the nonstructural genes.We concluded that greater variability in significant minor populations occurred as the epidemic progressed, particularly in the nonstructural genes, with higher variability observed in secondary infection cases.Remarkably, for the first time significant intrahost genetic variation was demonstrated within the same patient during the course of secondary infection with DENV-1/DENV-3, including changes in structural proteins.
Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba firstname.lastname@example.org.Show MeSH
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Mentions: The Bayesian phylogenetic tree constructed with complete polyprotein sequences indicated that all Cuban isolates collected during the 2001-2002 epidemic grouped within genotype III, introduced in Latin America since 1994 (Fig. 1). Therefore, as expected, the Nicaraguan strain (NI_BID_V2420_1994) isolated around this time was located at the base of the Latin American group. All major nodes were statistically reliable according to the estimates of posterior probability. The phylogenetic tree further suggested that two lineages were circulating in Havana. It was noticeable that most Cuban isolates (20 isolates) representing the main lineage formed an independent monophyletic subgroup, closely related to Peruvian and Ecuadorian isolates from 2000 to 2002, but two isolates from the beginning of the epidemic(Cuba_118_2001 and Cuba_167_2001) appeared slightly distant, closely related to Venezuelan isolates from 2001.
Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba email@example.com.