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Increasing Clinical Severity during a Dengue Virus Type 3 Cuban Epidemic: Deep Sequencing of Evolving Viral Populations.

Rodriguez-Roche R, Blanc H, Bordería AV, Díaz G, Henningsson R, Gonzalez D, Santana E, Alvarez M, Castro O, Fontes M, Vignuzzi M, Guzman MG - J. Virol. (2016)

Bottom Line: In conclusion, greater variability was detected during the epidemic's progression in terms of significant minority variants, particularly in the nonstructural genes.We concluded that greater variability in significant minor populations occurred as the epidemic progressed, particularly in the nonstructural genes, with higher variability observed in secondary infection cases.Remarkably, for the first time significant intrahost genetic variation was demonstrated within the same patient during the course of secondary infection with DENV-1/DENV-3, including changes in structural proteins.

View Article: PubMed Central - PubMed

Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba rosmari@ipk.sld.cu.

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Related in: MedlinePlus

Bayesian phylogeny of the DENV-3 polyprotein data set, including Cuban isolates from the 2001-2002 epidemic highlighted in gray. All horizontal branch lengths are drawn to scale. Bar, 0.02 substitutions per site. The tree is midpoint rooted for purposes of clarity only.
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Figure 1: Bayesian phylogeny of the DENV-3 polyprotein data set, including Cuban isolates from the 2001-2002 epidemic highlighted in gray. All horizontal branch lengths are drawn to scale. Bar, 0.02 substitutions per site. The tree is midpoint rooted for purposes of clarity only.

Mentions: The Bayesian phylogenetic tree constructed with complete polyprotein sequences indicated that all Cuban isolates collected during the 2001-2002 epidemic grouped within genotype III, introduced in Latin America since 1994 (Fig. 1). Therefore, as expected, the Nicaraguan strain (NI_BID_V2420_1994) isolated around this time was located at the base of the Latin American group. All major nodes were statistically reliable according to the estimates of posterior probability. The phylogenetic tree further suggested that two lineages were circulating in Havana. It was noticeable that most Cuban isolates (20 isolates) representing the main lineage formed an independent monophyletic subgroup, closely related to Peruvian and Ecuadorian isolates from 2000 to 2002, but two isolates from the beginning of the epidemic(Cuba_118_2001 and Cuba_167_2001) appeared slightly distant, closely related to Venezuelan isolates from 2001.


Increasing Clinical Severity during a Dengue Virus Type 3 Cuban Epidemic: Deep Sequencing of Evolving Viral Populations.

Rodriguez-Roche R, Blanc H, Bordería AV, Díaz G, Henningsson R, Gonzalez D, Santana E, Alvarez M, Castro O, Fontes M, Vignuzzi M, Guzman MG - J. Virol. (2016)

Bayesian phylogeny of the DENV-3 polyprotein data set, including Cuban isolates from the 2001-2002 epidemic highlighted in gray. All horizontal branch lengths are drawn to scale. Bar, 0.02 substitutions per site. The tree is midpoint rooted for purposes of clarity only.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836355&req=5

Figure 1: Bayesian phylogeny of the DENV-3 polyprotein data set, including Cuban isolates from the 2001-2002 epidemic highlighted in gray. All horizontal branch lengths are drawn to scale. Bar, 0.02 substitutions per site. The tree is midpoint rooted for purposes of clarity only.
Mentions: The Bayesian phylogenetic tree constructed with complete polyprotein sequences indicated that all Cuban isolates collected during the 2001-2002 epidemic grouped within genotype III, introduced in Latin America since 1994 (Fig. 1). Therefore, as expected, the Nicaraguan strain (NI_BID_V2420_1994) isolated around this time was located at the base of the Latin American group. All major nodes were statistically reliable according to the estimates of posterior probability. The phylogenetic tree further suggested that two lineages were circulating in Havana. It was noticeable that most Cuban isolates (20 isolates) representing the main lineage formed an independent monophyletic subgroup, closely related to Peruvian and Ecuadorian isolates from 2000 to 2002, but two isolates from the beginning of the epidemic(Cuba_118_2001 and Cuba_167_2001) appeared slightly distant, closely related to Venezuelan isolates from 2001.

Bottom Line: In conclusion, greater variability was detected during the epidemic's progression in terms of significant minority variants, particularly in the nonstructural genes.We concluded that greater variability in significant minor populations occurred as the epidemic progressed, particularly in the nonstructural genes, with higher variability observed in secondary infection cases.Remarkably, for the first time significant intrahost genetic variation was demonstrated within the same patient during the course of secondary infection with DENV-1/DENV-3, including changes in structural proteins.

View Article: PubMed Central - PubMed

Affiliation: Virology Department, Pedro Kouri Institute of Tropical Medicine, PAHO/WHO Collaborating Center for the Study of Dengue and Its Vector, Havana, Cuba rosmari@ipk.sld.cu.

Show MeSH
Related in: MedlinePlus