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MicroRNA-19b-3p Modulates Japanese Encephalitis Virus-Mediated Inflammation via Targeting RNF11.

Ashraf U, Zhu B, Ye J, Wan S, Nie Y, Chen Z, Cui M, Wang C, Duan X, Zhang H, Chen H, Cao S - J. Virol. (2016)

Bottom Line: Japanese encephalitis virus (JEV) can invade the central nervous system and consequently induce neuroinflammation, which is characterized by profound neuronal cell damage accompanied by astrogliosis and microgliosis.The pathological features of JEV-induced encephalitis are inflammatory reactions and neurological diseases resulting from glia activation.The present study reveals that miR-19b-3p targets ring finger protein 11 in glia and promotes inflammatory cytokine production by enhancing nuclear factor kappa B activity in these cells.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.

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RNF11 expression is downregulated after JEV infection. (A) U251 cells were infected with JEV at an MOI of 5 for the indicated times, and then RNF11 mRNA expression levels were determined by quantitative real-time PCR. (B) U251 cells were infected with JEV at an MOI of 5 for the indicated times, and then RNF11 protein levels were determined with immunoblotting. (C and D) BV2 cells were infected with JEV at an MOI of 5 for the indicated times, and then RNF11 mRNA (C) and protein (D) expression levels were determined by quantitative real-time PCR and immunoblotting, respectively. Data represent means ± SD from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Protein levels were quantified with immunoblot scanning and normalized to the amount of GAPDH expression.
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Figure 5: RNF11 expression is downregulated after JEV infection. (A) U251 cells were infected with JEV at an MOI of 5 for the indicated times, and then RNF11 mRNA expression levels were determined by quantitative real-time PCR. (B) U251 cells were infected with JEV at an MOI of 5 for the indicated times, and then RNF11 protein levels were determined with immunoblotting. (C and D) BV2 cells were infected with JEV at an MOI of 5 for the indicated times, and then RNF11 mRNA (C) and protein (D) expression levels were determined by quantitative real-time PCR and immunoblotting, respectively. Data represent means ± SD from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Protein levels were quantified with immunoblot scanning and normalized to the amount of GAPDH expression.

Mentions: To study the effect of JEV infection on RNF11, the time-dependent expression patterns of RNF11 mRNA (Fig. 5A) and protein (Fig. 5B) in U251 cells following JEV infection were studied. Significant downregulation of RNF11 mRNA and protein levels at 12, 24, and 36 h postinfection was observed. Furthermore, RNF11 mRNA and protein expression levels were also determined in JEV-infected BV2 cells (Fig. 5C and D). The results were concordant with those in JEV-infected U251 cells. Thus, these data demonstrate that RNF11 expression is downregulated upon JEV infection.


MicroRNA-19b-3p Modulates Japanese Encephalitis Virus-Mediated Inflammation via Targeting RNF11.

Ashraf U, Zhu B, Ye J, Wan S, Nie Y, Chen Z, Cui M, Wang C, Duan X, Zhang H, Chen H, Cao S - J. Virol. (2016)

RNF11 expression is downregulated after JEV infection. (A) U251 cells were infected with JEV at an MOI of 5 for the indicated times, and then RNF11 mRNA expression levels were determined by quantitative real-time PCR. (B) U251 cells were infected with JEV at an MOI of 5 for the indicated times, and then RNF11 protein levels were determined with immunoblotting. (C and D) BV2 cells were infected with JEV at an MOI of 5 for the indicated times, and then RNF11 mRNA (C) and protein (D) expression levels were determined by quantitative real-time PCR and immunoblotting, respectively. Data represent means ± SD from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Protein levels were quantified with immunoblot scanning and normalized to the amount of GAPDH expression.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4836334&req=5

Figure 5: RNF11 expression is downregulated after JEV infection. (A) U251 cells were infected with JEV at an MOI of 5 for the indicated times, and then RNF11 mRNA expression levels were determined by quantitative real-time PCR. (B) U251 cells were infected with JEV at an MOI of 5 for the indicated times, and then RNF11 protein levels were determined with immunoblotting. (C and D) BV2 cells were infected with JEV at an MOI of 5 for the indicated times, and then RNF11 mRNA (C) and protein (D) expression levels were determined by quantitative real-time PCR and immunoblotting, respectively. Data represent means ± SD from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001. Protein levels were quantified with immunoblot scanning and normalized to the amount of GAPDH expression.
Mentions: To study the effect of JEV infection on RNF11, the time-dependent expression patterns of RNF11 mRNA (Fig. 5A) and protein (Fig. 5B) in U251 cells following JEV infection were studied. Significant downregulation of RNF11 mRNA and protein levels at 12, 24, and 36 h postinfection was observed. Furthermore, RNF11 mRNA and protein expression levels were also determined in JEV-infected BV2 cells (Fig. 5C and D). The results were concordant with those in JEV-infected U251 cells. Thus, these data demonstrate that RNF11 expression is downregulated upon JEV infection.

Bottom Line: Japanese encephalitis virus (JEV) can invade the central nervous system and consequently induce neuroinflammation, which is characterized by profound neuronal cell damage accompanied by astrogliosis and microgliosis.The pathological features of JEV-induced encephalitis are inflammatory reactions and neurological diseases resulting from glia activation.The present study reveals that miR-19b-3p targets ring finger protein 11 in glia and promotes inflammatory cytokine production by enhancing nuclear factor kappa B activity in these cells.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Laboratory of Animal Virology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, Hubei, People's Republic of China.

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Related in: MedlinePlus