Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity.
Bottom Line: In addition, we found that GBP1 was upregulated at the transcriptional level in CSFV-infected PK-15 cells and in various organs of CSFV-infected pigs.We showed further that the NS5A-GBP1 interaction inhibited GTPase activity, which was critical for its antiviral effect.The K51 of GBP1, critical for its GTPase activity, is essential for the antiviral action of GBP1 against CSFV replication, and the binding of the NS5A protein to GBP1 antagonizes the GTPase activity and thus the antiviral effect.
Affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.Show MeSH
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Mentions: To examine the expression of GBP1 following CSFV infection, PK-15 cells were infected with Shimen and examined by qRT-PCR. As controls, PK-15 cells were treated with different amounts of IFN-β. As expected, the expression of GBP1 was upregulated by IFN-β in a dose-dependent manner (Fig. 3A). Similar results were observed in PK-15 cells infected with Shimen (Fig. 3B). We also tested the expression of GBP1 in different organs of pigs infected with Shimen. The results showed that CSFV infection induced GBP1 expression in target organs for CSFV, including the spleen, lung, kidney, and tonsils (Fig. 3C).
Affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.