Guanylate-Binding Protein 1, an Interferon-Induced GTPase, Exerts an Antiviral Activity against Classical Swine Fever Virus Depending on Its GTPase Activity.
Bottom Line: In addition, we found that GBP1 was upregulated at the transcriptional level in CSFV-infected PK-15 cells and in various organs of CSFV-infected pigs.We showed further that the NS5A-GBP1 interaction inhibited GTPase activity, which was critical for its antiviral effect.The K51 of GBP1, critical for its GTPase activity, is essential for the antiviral action of GBP1 against CSFV replication, and the binding of the NS5A protein to GBP1 antagonizes the GTPase activity and thus the antiviral effect.
Affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.Show MeSH
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Mentions: Now that overexpression of GBP1 was found to inhibit rCSFV-Fluc replication (Fig. 1B), the antiviral activity of GBP1 against CSFV was examined in PK-GBP1 cells. Virus titers in culture supernatants of PK-GBP1 cells were decreased (85.2% ± 3.1%) (Fig. 2A), and the number of viral genomic copies in PK-GBP1 cells was reduced (87.2% ± 2.8%) (Fig. 2B), compared to that in PK-EGFP cells at 48 hpi. Furthermore, the expression level of Npro protein was lower in CSFV-infected PK-GBP1 cells than in CSFV-infected PK-EGFP cells (Fig. 2C).
Affiliation: State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, China.