Limits...
Dynamic and quantitative assessment of blood coagulation using optical coherence elastography.

Xu X, Zhu J, Chen Z - Sci Rep (2016)

Bottom Line: In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system.During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave.The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.

ABSTRACT
Reliable clot diagnostic systems are needed for directing treatment in a broad spectrum of cardiovascular diseases and coagulopathy. Here, we report on non-contact measurement of elastic modulus for dynamic and quantitative assessment of whole blood coagulation using acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE). In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system. During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave. Consequently, dynamic blood coagulation status can be measured quantitatively by relating the velocity of the shear wave with clinically relevant coagulation metrics, including reaction time, clot formation kinetics and maximum shear modulus. The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.

No MeSH data available.


Related in: MedlinePlus

The schematic of the ARFOE-OCE system, including the swept source OCT unit and ultrasonic excitation unit.The vibration perpendicular to the OCT detection direction is detected by a Doppler variance method, and the shear wave propagating parallel to the OCT detection direction is measured by M-scans. The blood is filled to a box with a window of plastic wrap for the ultrasound wave passing. The box and the transducer are immersed in water for impedance matching of the ultrasound wave into the blood sample. Only the container contacts with the blood sample.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4836302&req=5

f5: The schematic of the ARFOE-OCE system, including the swept source OCT unit and ultrasonic excitation unit.The vibration perpendicular to the OCT detection direction is detected by a Doppler variance method, and the shear wave propagating parallel to the OCT detection direction is measured by M-scans. The blood is filled to a box with a window of plastic wrap for the ultrasound wave passing. The box and the transducer are immersed in water for impedance matching of the ultrasound wave into the blood sample. Only the container contacts with the blood sample.

Mentions: The experimental setup for the ARFOE-OCE is shown in Fig. 5. For ARF generation, an ultrasonic transducer with a resonant frequency of 4.5 MHz is driven by the amplified sine wave at 160 V peak-to-peak. The blood is filled to a box with a window of plastic wrap for the ultrasound wave passing. The box and the transducer are immersed in water for impedance matching of the ultrasound wave into the blood sample. The OCT system is based on a swept source with a central wavelength of 1310 nm and an A-line speed of 50 kHz. The light from the laser source is split into the sample arm and the reference arm with a power of 90% and 10%, respectively. For shear wave detection, 1000 A-lines at a rate of 50 kHz, corresponding to total time of 20.0 ms, are involved in one OCT M-scan, and the ARF excitation is applied between the 101st A-line to the 150th A-line corresponding to 1.0 ms emission. In this system, the ARF-induced vibration is perpendicular to the OCT beam, and the shear wave propagates along the OCT beam. Only the container contacts with the blood sample. The acoustic excitation system and optical detection system will not contact the blood sample.


Dynamic and quantitative assessment of blood coagulation using optical coherence elastography.

Xu X, Zhu J, Chen Z - Sci Rep (2016)

The schematic of the ARFOE-OCE system, including the swept source OCT unit and ultrasonic excitation unit.The vibration perpendicular to the OCT detection direction is detected by a Doppler variance method, and the shear wave propagating parallel to the OCT detection direction is measured by M-scans. The blood is filled to a box with a window of plastic wrap for the ultrasound wave passing. The box and the transducer are immersed in water for impedance matching of the ultrasound wave into the blood sample. Only the container contacts with the blood sample.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836302&req=5

f5: The schematic of the ARFOE-OCE system, including the swept source OCT unit and ultrasonic excitation unit.The vibration perpendicular to the OCT detection direction is detected by a Doppler variance method, and the shear wave propagating parallel to the OCT detection direction is measured by M-scans. The blood is filled to a box with a window of plastic wrap for the ultrasound wave passing. The box and the transducer are immersed in water for impedance matching of the ultrasound wave into the blood sample. Only the container contacts with the blood sample.
Mentions: The experimental setup for the ARFOE-OCE is shown in Fig. 5. For ARF generation, an ultrasonic transducer with a resonant frequency of 4.5 MHz is driven by the amplified sine wave at 160 V peak-to-peak. The blood is filled to a box with a window of plastic wrap for the ultrasound wave passing. The box and the transducer are immersed in water for impedance matching of the ultrasound wave into the blood sample. The OCT system is based on a swept source with a central wavelength of 1310 nm and an A-line speed of 50 kHz. The light from the laser source is split into the sample arm and the reference arm with a power of 90% and 10%, respectively. For shear wave detection, 1000 A-lines at a rate of 50 kHz, corresponding to total time of 20.0 ms, are involved in one OCT M-scan, and the ARF excitation is applied between the 101st A-line to the 150th A-line corresponding to 1.0 ms emission. In this system, the ARF-induced vibration is perpendicular to the OCT beam, and the shear wave propagates along the OCT beam. Only the container contacts with the blood sample. The acoustic excitation system and optical detection system will not contact the blood sample.

Bottom Line: In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system.During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave.The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.

ABSTRACT
Reliable clot diagnostic systems are needed for directing treatment in a broad spectrum of cardiovascular diseases and coagulopathy. Here, we report on non-contact measurement of elastic modulus for dynamic and quantitative assessment of whole blood coagulation using acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE). In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system. During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave. Consequently, dynamic blood coagulation status can be measured quantitatively by relating the velocity of the shear wave with clinically relevant coagulation metrics, including reaction time, clot formation kinetics and maximum shear modulus. The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.

No MeSH data available.


Related in: MedlinePlus