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Dynamic and quantitative assessment of blood coagulation using optical coherence elastography.

Xu X, Zhu J, Chen Z - Sci Rep (2016)

Bottom Line: In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system.During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave.The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.

ABSTRACT
Reliable clot diagnostic systems are needed for directing treatment in a broad spectrum of cardiovascular diseases and coagulopathy. Here, we report on non-contact measurement of elastic modulus for dynamic and quantitative assessment of whole blood coagulation using acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE). In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system. During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave. Consequently, dynamic blood coagulation status can be measured quantitatively by relating the velocity of the shear wave with clinically relevant coagulation metrics, including reaction time, clot formation kinetics and maximum shear modulus. The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.

No MeSH data available.


Related in: MedlinePlus

Comparison of shear modulus change with and without the fibrinogen during porcine whole blood coagulation.The additional fibrinogen changes the clot formation rate and increases the clot stiffness. No significant difference in the reaction time is measured between the control group and group spiked with fibrinogen.
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f4: Comparison of shear modulus change with and without the fibrinogen during porcine whole blood coagulation.The additional fibrinogen changes the clot formation rate and increases the clot stiffness. No significant difference in the reaction time is measured between the control group and group spiked with fibrinogen.

Mentions: The capability of ARFOE-OCE for characterizing the different coagulation process spiked by fibrinogen is evaluated and shown in Fig. 4. The tests are performed using porcine whole blood without fibrinogen (n = 3) and porcine whole blood spiked with fibrinogen (n = 3). The addition of fibrinogen does not significantly affect the reaction time (P > 0.05) but significant differences are observed between the control group and blood samples with additional 4 g/L fibrinogen in the clot formation kinetics (P < 0.01) and maximum shear modulus (P < 0.01), respectively. The values of reaction time, clot formation kinetics and maximum shear modulus for two groups of blood samples are presented in Table 2. The presence of additional fibrinogen significantly changes the clot formation rate and increases the peak firmness of clot.


Dynamic and quantitative assessment of blood coagulation using optical coherence elastography.

Xu X, Zhu J, Chen Z - Sci Rep (2016)

Comparison of shear modulus change with and without the fibrinogen during porcine whole blood coagulation.The additional fibrinogen changes the clot formation rate and increases the clot stiffness. No significant difference in the reaction time is measured between the control group and group spiked with fibrinogen.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836302&req=5

f4: Comparison of shear modulus change with and without the fibrinogen during porcine whole blood coagulation.The additional fibrinogen changes the clot formation rate and increases the clot stiffness. No significant difference in the reaction time is measured between the control group and group spiked with fibrinogen.
Mentions: The capability of ARFOE-OCE for characterizing the different coagulation process spiked by fibrinogen is evaluated and shown in Fig. 4. The tests are performed using porcine whole blood without fibrinogen (n = 3) and porcine whole blood spiked with fibrinogen (n = 3). The addition of fibrinogen does not significantly affect the reaction time (P > 0.05) but significant differences are observed between the control group and blood samples with additional 4 g/L fibrinogen in the clot formation kinetics (P < 0.01) and maximum shear modulus (P < 0.01), respectively. The values of reaction time, clot formation kinetics and maximum shear modulus for two groups of blood samples are presented in Table 2. The presence of additional fibrinogen significantly changes the clot formation rate and increases the peak firmness of clot.

Bottom Line: In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system.During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave.The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.

ABSTRACT
Reliable clot diagnostic systems are needed for directing treatment in a broad spectrum of cardiovascular diseases and coagulopathy. Here, we report on non-contact measurement of elastic modulus for dynamic and quantitative assessment of whole blood coagulation using acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE). In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system. During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave. Consequently, dynamic blood coagulation status can be measured quantitatively by relating the velocity of the shear wave with clinically relevant coagulation metrics, including reaction time, clot formation kinetics and maximum shear modulus. The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.

No MeSH data available.


Related in: MedlinePlus