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Dynamic and quantitative assessment of blood coagulation using optical coherence elastography.

Xu X, Zhu J, Chen Z - Sci Rep (2016)

Bottom Line: In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system.During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave.The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.

ABSTRACT
Reliable clot diagnostic systems are needed for directing treatment in a broad spectrum of cardiovascular diseases and coagulopathy. Here, we report on non-contact measurement of elastic modulus for dynamic and quantitative assessment of whole blood coagulation using acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE). In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system. During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave. Consequently, dynamic blood coagulation status can be measured quantitatively by relating the velocity of the shear wave with clinically relevant coagulation metrics, including reaction time, clot formation kinetics and maximum shear modulus. The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.

No MeSH data available.


Related in: MedlinePlus

OCT and Doppler variance images for the blood coagulation measurement.(a) The OCT image at the beginning of the measurement. The ARF focus is moved downwards by a mechanical lifting stage at a step of 1mm for each measurement and the OCT M-scan is applied at the position indicated by the white arrow. (b) The Doppler variance images at four typical time points successively using three ARF focus positions. In the process of blood coagulation, the wave occurrence time of the shear wave becomes shorter, indicating a faster shear wave velocity using each of the ARF focus positions.
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f1: OCT and Doppler variance images for the blood coagulation measurement.(a) The OCT image at the beginning of the measurement. The ARF focus is moved downwards by a mechanical lifting stage at a step of 1mm for each measurement and the OCT M-scan is applied at the position indicated by the white arrow. (b) The Doppler variance images at four typical time points successively using three ARF focus positions. In the process of blood coagulation, the wave occurrence time of the shear wave becomes shorter, indicating a faster shear wave velocity using each of the ARF focus positions.

Mentions: First, we investigate the feasibility of the ARFOE-OCE for measuring the shear modulus in the clotting blood. Time-course coagulation of the citrated porcine whole blood is investigated. Figure 1a shows the OCT image in a B-mode at 3 min following the recalcification of blood coagulation. For the shear wave detection, 43 M-scans are captured at the position indicated by the white arrow in Fig. 1a using each of three ARF excitation positions with a spacing of 1 mm. Figure 1b and Supplementary Videos 1,2–3 shows the Doppler variance images at different points successively using three ARF focus positions. At 3 min, no obvious vibration is detected; thus, no shear wave propagates to the OCT imaging area. After 8 min, the transverse vibration of the blood sample is detectable, which is generated by a shear wave propagating from the ARF focus. In the process of blood coagulation, the wave occurrence time becomes shorter, indicating a faster shear wave velocity using each of the ARF focus positions.


Dynamic and quantitative assessment of blood coagulation using optical coherence elastography.

Xu X, Zhu J, Chen Z - Sci Rep (2016)

OCT and Doppler variance images for the blood coagulation measurement.(a) The OCT image at the beginning of the measurement. The ARF focus is moved downwards by a mechanical lifting stage at a step of 1mm for each measurement and the OCT M-scan is applied at the position indicated by the white arrow. (b) The Doppler variance images at four typical time points successively using three ARF focus positions. In the process of blood coagulation, the wave occurrence time of the shear wave becomes shorter, indicating a faster shear wave velocity using each of the ARF focus positions.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836302&req=5

f1: OCT and Doppler variance images for the blood coagulation measurement.(a) The OCT image at the beginning of the measurement. The ARF focus is moved downwards by a mechanical lifting stage at a step of 1mm for each measurement and the OCT M-scan is applied at the position indicated by the white arrow. (b) The Doppler variance images at four typical time points successively using three ARF focus positions. In the process of blood coagulation, the wave occurrence time of the shear wave becomes shorter, indicating a faster shear wave velocity using each of the ARF focus positions.
Mentions: First, we investigate the feasibility of the ARFOE-OCE for measuring the shear modulus in the clotting blood. Time-course coagulation of the citrated porcine whole blood is investigated. Figure 1a shows the OCT image in a B-mode at 3 min following the recalcification of blood coagulation. For the shear wave detection, 43 M-scans are captured at the position indicated by the white arrow in Fig. 1a using each of three ARF excitation positions with a spacing of 1 mm. Figure 1b and Supplementary Videos 1,2–3 shows the Doppler variance images at different points successively using three ARF focus positions. At 3 min, no obvious vibration is detected; thus, no shear wave propagates to the OCT imaging area. After 8 min, the transverse vibration of the blood sample is detectable, which is generated by a shear wave propagating from the ARF focus. In the process of blood coagulation, the wave occurrence time becomes shorter, indicating a faster shear wave velocity using each of the ARF focus positions.

Bottom Line: In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system.During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave.The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.

View Article: PubMed Central - PubMed

Affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, Zhejiang 310018, China.

ABSTRACT
Reliable clot diagnostic systems are needed for directing treatment in a broad spectrum of cardiovascular diseases and coagulopathy. Here, we report on non-contact measurement of elastic modulus for dynamic and quantitative assessment of whole blood coagulation using acoustic radiation force orthogonal excitation optical coherence elastography (ARFOE-OCE). In this system, acoustic radiation force (ARF) is produced by a remote ultrasonic transducer, and a shear wave induced by ARF excitation is detected by the optical coherence tomography (OCT) system. During porcine whole blood coagulation, changes in the elastic property of the clots increase the shear modulus of the sample, altering the propagating velocity of the shear wave. Consequently, dynamic blood coagulation status can be measured quantitatively by relating the velocity of the shear wave with clinically relevant coagulation metrics, including reaction time, clot formation kinetics and maximum shear modulus. The results show that the ARFOE-OCE is sensitive to the clot formation kinetics and can differentiate the elastic properties of the recalcified porcine whole blood, blood added with kaolin as an activator, and blood spiked with fibrinogen.

No MeSH data available.


Related in: MedlinePlus