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Identification of (poly)phenol treatments that modulate the release of pro-inflammatory cytokines by human lymphocytes.

Ford CT, Richardson S, McArdle F, Lotito SB, Crozier A, McArdle A, Jackson MJ - Br. J. Nutr. (2016)

Bottom Line: We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes.A number of (poly)phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release.These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of pro-inflammatory mediators in people at risk of chronic inflammation.

View Article: PubMed Central - PubMed

Affiliation: 1Department of Musculoskeletal Biology,Institute of Ageing and Chronic Disease,University of Liverpool,Liverpool L7 8TX,UK.

ABSTRACT
Diets rich in fruits and vegetables (FV), which contain (poly)phenols, protect against age-related inflammation and chronic diseases. T-lymphocytes contribute to systemic cytokine production and are modulated by FV intake. Little is known about the relative potency of different (poly)phenols in modulating cytokine release by lymphocytes. We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes. Test compounds were incubated with Jurkat cells for 48 h at 1 and 30 µm, with or without phorbol ester treatment at 24 h to induce cytokine release. Three test compounds that reduced cytokine release were further incubated with primary lymphocytes at 0·2 and 1 µm for 24 h, with lipopolysaccharide added at 5 h. Cytokine release was measured, and generation of H2O2 by test compounds was determined to assess any potential correlations with cytokine release. A number of (poly)phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release. Resveratrol, isorhamnetin, curcumin, vanillic acid and specific (poly)phenol mixtures reduced pro-inflammatory cytokine release from T-lymphocytes, and there was evidence for interaction between (poly)phenols to further modulate cytokine release. The release of interferon-γ induced protein 10 by primary lymphocytes was significantly reduced following treatment with 1 µm isorhamnetin (P<0·05). These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of pro-inflammatory mediators in people at risk of chronic inflammation.

No MeSH data available.


Related in: MedlinePlus

Cytokine release by lymphocytes from a healthy donor following treatment for 24 hwith the polyphenols resveratrol, isorhamnetin and curcumin with the addition of 20ng/ml lipopolysaccharide (LPS) at the 5-h time point. Isorhamnetin at 1 µmol/lsignificantly reduced interferon-γ induced protein 10 (IP-10)release compared with the matched DMSO vehicle control(P<0·05; one-way ANOVA with Dunnett’s posthoc test). *Groups that significantly differed from the control group(one-way ANOVA with Dunnett’s post hoc test;P<0·05). There were no significant differences betweengroups for other cytokines. Data for the other cytokines are available in the OnlineSupplementary Fig. S2. LVC, LPS and vehicle control; 0·2 and 1 denote concentrationsin µmol/l.
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fig5: Cytokine release by lymphocytes from a healthy donor following treatment for 24 hwith the polyphenols resveratrol, isorhamnetin and curcumin with the addition of 20ng/ml lipopolysaccharide (LPS) at the 5-h time point. Isorhamnetin at 1 µmol/lsignificantly reduced interferon-γ induced protein 10 (IP-10)release compared with the matched DMSO vehicle control(P<0·05; one-way ANOVA with Dunnett’s posthoc test). *Groups that significantly differed from the control group(one-way ANOVA with Dunnett’s post hoc test;P<0·05). There were no significant differences betweengroups for other cytokines. Data for the other cytokines are available in the OnlineSupplementary Fig. S2. LVC, LPS and vehicle control; 0·2 and 1 denote concentrationsin µmol/l.

Mentions: We investigated the polyphenols resveratrol, isorhamnetin and curcumin, which had beenidentified to be the most effective in reducing cytokine release from among the panel of(poly)phenols that we screened in Jurkat CD4+ T-lymphocytes, to determinewhether their inhibitory effects on pro-inflammatory cytokine release were sustained inPBMC-derived human lymphocytes (Fig. 5). Theresults suggest trends (i.e. P<0·09) towards decreased IL-6,interferon-γ induced protein 10 (IP-10) and TNFαrelease following treatment with resveratrol, isorhamnetin and curcumin at 0·2 or 1µm. The reduction in IP-10 release after treatment with 1 µmisorhamnetin was statistically significant (P<0·05, one-way ANOVAwith Dunnett’s post hoc test).Fig. 5


Identification of (poly)phenol treatments that modulate the release of pro-inflammatory cytokines by human lymphocytes.

Ford CT, Richardson S, McArdle F, Lotito SB, Crozier A, McArdle A, Jackson MJ - Br. J. Nutr. (2016)

Cytokine release by lymphocytes from a healthy donor following treatment for 24 hwith the polyphenols resveratrol, isorhamnetin and curcumin with the addition of 20ng/ml lipopolysaccharide (LPS) at the 5-h time point. Isorhamnetin at 1 µmol/lsignificantly reduced interferon-γ induced protein 10 (IP-10)release compared with the matched DMSO vehicle control(P<0·05; one-way ANOVA with Dunnett’s posthoc test). *Groups that significantly differed from the control group(one-way ANOVA with Dunnett’s post hoc test;P<0·05). There were no significant differences betweengroups for other cytokines. Data for the other cytokines are available in the OnlineSupplementary Fig. S2. LVC, LPS and vehicle control; 0·2 and 1 denote concentrationsin µmol/l.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4836295&req=5

fig5: Cytokine release by lymphocytes from a healthy donor following treatment for 24 hwith the polyphenols resveratrol, isorhamnetin and curcumin with the addition of 20ng/ml lipopolysaccharide (LPS) at the 5-h time point. Isorhamnetin at 1 µmol/lsignificantly reduced interferon-γ induced protein 10 (IP-10)release compared with the matched DMSO vehicle control(P<0·05; one-way ANOVA with Dunnett’s posthoc test). *Groups that significantly differed from the control group(one-way ANOVA with Dunnett’s post hoc test;P<0·05). There were no significant differences betweengroups for other cytokines. Data for the other cytokines are available in the OnlineSupplementary Fig. S2. LVC, LPS and vehicle control; 0·2 and 1 denote concentrationsin µmol/l.
Mentions: We investigated the polyphenols resveratrol, isorhamnetin and curcumin, which had beenidentified to be the most effective in reducing cytokine release from among the panel of(poly)phenols that we screened in Jurkat CD4+ T-lymphocytes, to determinewhether their inhibitory effects on pro-inflammatory cytokine release were sustained inPBMC-derived human lymphocytes (Fig. 5). Theresults suggest trends (i.e. P<0·09) towards decreased IL-6,interferon-γ induced protein 10 (IP-10) and TNFαrelease following treatment with resveratrol, isorhamnetin and curcumin at 0·2 or 1µm. The reduction in IP-10 release after treatment with 1 µmisorhamnetin was statistically significant (P<0·05, one-way ANOVAwith Dunnett’s post hoc test).Fig. 5

Bottom Line: We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes.A number of (poly)phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release.These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of pro-inflammatory mediators in people at risk of chronic inflammation.

View Article: PubMed Central - PubMed

Affiliation: 1Department of Musculoskeletal Biology,Institute of Ageing and Chronic Disease,University of Liverpool,Liverpool L7 8TX,UK.

ABSTRACT
Diets rich in fruits and vegetables (FV), which contain (poly)phenols, protect against age-related inflammation and chronic diseases. T-lymphocytes contribute to systemic cytokine production and are modulated by FV intake. Little is known about the relative potency of different (poly)phenols in modulating cytokine release by lymphocytes. We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes. Test compounds were incubated with Jurkat cells for 48 h at 1 and 30 µm, with or without phorbol ester treatment at 24 h to induce cytokine release. Three test compounds that reduced cytokine release were further incubated with primary lymphocytes at 0·2 and 1 µm for 24 h, with lipopolysaccharide added at 5 h. Cytokine release was measured, and generation of H2O2 by test compounds was determined to assess any potential correlations with cytokine release. A number of (poly)phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release. Resveratrol, isorhamnetin, curcumin, vanillic acid and specific (poly)phenol mixtures reduced pro-inflammatory cytokine release from T-lymphocytes, and there was evidence for interaction between (poly)phenols to further modulate cytokine release. The release of interferon-γ induced protein 10 by primary lymphocytes was significantly reduced following treatment with 1 µm isorhamnetin (P<0·05). These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of pro-inflammatory mediators in people at risk of chronic inflammation.

No MeSH data available.


Related in: MedlinePlus