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Identification of (poly)phenol treatments that modulate the release of pro-inflammatory cytokines by human lymphocytes.

Ford CT, Richardson S, McArdle F, Lotito SB, Crozier A, McArdle A, Jackson MJ - Br. J. Nutr. (2016)

Bottom Line: We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes.A number of (poly)phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release.These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of pro-inflammatory mediators in people at risk of chronic inflammation.

View Article: PubMed Central - PubMed

Affiliation: 1Department of Musculoskeletal Biology,Institute of Ageing and Chronic Disease,University of Liverpool,Liverpool L7 8TX,UK.

ABSTRACT
Diets rich in fruits and vegetables (FV), which contain (poly)phenols, protect against age-related inflammation and chronic diseases. T-lymphocytes contribute to systemic cytokine production and are modulated by FV intake. Little is known about the relative potency of different (poly)phenols in modulating cytokine release by lymphocytes. We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes. Test compounds were incubated with Jurkat cells for 48 h at 1 and 30 µm, with or without phorbol ester treatment at 24 h to induce cytokine release. Three test compounds that reduced cytokine release were further incubated with primary lymphocytes at 0·2 and 1 µm for 24 h, with lipopolysaccharide added at 5 h. Cytokine release was measured, and generation of H2O2 by test compounds was determined to assess any potential correlations with cytokine release. A number of (poly)phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release. Resveratrol, isorhamnetin, curcumin, vanillic acid and specific (poly)phenol mixtures reduced pro-inflammatory cytokine release from T-lymphocytes, and there was evidence for interaction between (poly)phenols to further modulate cytokine release. The release of interferon-γ induced protein 10 by primary lymphocytes was significantly reduced following treatment with 1 µm isorhamnetin (P<0·05). These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of pro-inflammatory mediators in people at risk of chronic inflammation.

No MeSH data available.


Related in: MedlinePlus

Heat map showing the effects of phenolic acids on cytokine release and growth byJurkat CD4+ T-lymphocytes. Compounds are ordered by molecular weight fromthe lowest weight (top) to the highest weight (bottom). Data are presented aspercentage differences from matched vehicle controls following 48 h of treatment.Treatment and control experiments were performed with or without 25 ng/ml phorbol12-myristate 13-acetate (PMA) and 5 µg/ml phytohaemagglutinin (PHA) stimulation at24 h. TNFα could not be measured in the absence of PMA/PHAstimulation. * Mean value was significantly different compared with vehicle controls(P<0·05; one-way ANOVA with Dunnett’s posthoc test). No significant effects were observed following treatment withcaffeic acid, ferulic acid, isoferulic acid, 5-(3'-hydroxyphenyl) propionic acid,5-(3',4'-dihydroxyphenyl) propionic acid, 5-(3'-methoxy-4'-hydroxyphenyl) propionicacid, homoprotocatechuic acid, 3-(4' hydroxyphenyl) lactic acid, hippuric acid or4'-hydroxyhippuric acid. Examples of the data from which these heat maps are derivedare provided in the online Supplementary data to allow an assessment of thevariability observed in these studies.
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fig2: Heat map showing the effects of phenolic acids on cytokine release and growth byJurkat CD4+ T-lymphocytes. Compounds are ordered by molecular weight fromthe lowest weight (top) to the highest weight (bottom). Data are presented aspercentage differences from matched vehicle controls following 48 h of treatment.Treatment and control experiments were performed with or without 25 ng/ml phorbol12-myristate 13-acetate (PMA) and 5 µg/ml phytohaemagglutinin (PHA) stimulation at24 h. TNFα could not be measured in the absence of PMA/PHAstimulation. * Mean value was significantly different compared with vehicle controls(P<0·05; one-way ANOVA with Dunnett’s posthoc test). No significant effects were observed following treatment withcaffeic acid, ferulic acid, isoferulic acid, 5-(3'-hydroxyphenyl) propionic acid,5-(3',4'-dihydroxyphenyl) propionic acid, 5-(3'-methoxy-4'-hydroxyphenyl) propionicacid, homoprotocatechuic acid, 3-(4' hydroxyphenyl) lactic acid, hippuric acid or4'-hydroxyhippuric acid. Examples of the data from which these heat maps are derivedare provided in the online Supplementary data to allow an assessment of thevariability observed in these studies.

Mentions: After treatment with colonic catabolites of polyphenols (phenolic acids) for 48 h, wemeasured the density of viable Jurkat CD4+ T-lymphocytes in culture and theconcentrations of the pro-inflammatory cytokines IL-2, IL-8 and TNFα inthe cell culture media. Comparisons between (poly)phenol treatments and vehicle controlsrevealed multiple effects on growth and cytokine release (Fig. 2). The low molecular weight (110–139 g/mol) catabolites catechol,phloroglucinol and 4-hydroxybenzoic acid caused significant declines in cell number(P<0·05; one-way ANOVA with Dunnett’s post hoctest) and significantly induced release of IL-2, IL-8 and TNFα(P<0·05; one-way ANOVA with Dunnett’s post hoctest), whereas tyrosol significantly decreased IL-2 and IL-8 release from non-stimulatedcells at 1 µmol/l (P<0·05; one-way ANOVA with Dunnett’spost hoc test). Mid-molecular weight catabolites (168–195 g/mol)reduced pro-inflammatory cytokine release, with significant reduction in IL-2 release fromnon-stimulated cells by 1 µmol/l 4'-hydroxymandelic acid and vanillic acid(P<0·05; one-way ANOVA with Dunnett’s post hoctest). Higher molecular weight test compounds (196–252 g/mol) showed mixed effects:feruloylglycine at 1 µmol/l significantly increased the release of IL-2 andTNFα, and isoferuloylglycine at 30 µmol/l significantly reduced IL-8release from PMA/PHA-stimulated Jurkat CD4+ T-lymphocytes(P<0·05; one-way ANOVA with Dunnett’s post hoctest).Fig. 2


Identification of (poly)phenol treatments that modulate the release of pro-inflammatory cytokines by human lymphocytes.

Ford CT, Richardson S, McArdle F, Lotito SB, Crozier A, McArdle A, Jackson MJ - Br. J. Nutr. (2016)

Heat map showing the effects of phenolic acids on cytokine release and growth byJurkat CD4+ T-lymphocytes. Compounds are ordered by molecular weight fromthe lowest weight (top) to the highest weight (bottom). Data are presented aspercentage differences from matched vehicle controls following 48 h of treatment.Treatment and control experiments were performed with or without 25 ng/ml phorbol12-myristate 13-acetate (PMA) and 5 µg/ml phytohaemagglutinin (PHA) stimulation at24 h. TNFα could not be measured in the absence of PMA/PHAstimulation. * Mean value was significantly different compared with vehicle controls(P<0·05; one-way ANOVA with Dunnett’s posthoc test). No significant effects were observed following treatment withcaffeic acid, ferulic acid, isoferulic acid, 5-(3'-hydroxyphenyl) propionic acid,5-(3',4'-dihydroxyphenyl) propionic acid, 5-(3'-methoxy-4'-hydroxyphenyl) propionicacid, homoprotocatechuic acid, 3-(4' hydroxyphenyl) lactic acid, hippuric acid or4'-hydroxyhippuric acid. Examples of the data from which these heat maps are derivedare provided in the online Supplementary data to allow an assessment of thevariability observed in these studies.
© Copyright Policy - open-access
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4836295&req=5

fig2: Heat map showing the effects of phenolic acids on cytokine release and growth byJurkat CD4+ T-lymphocytes. Compounds are ordered by molecular weight fromthe lowest weight (top) to the highest weight (bottom). Data are presented aspercentage differences from matched vehicle controls following 48 h of treatment.Treatment and control experiments were performed with or without 25 ng/ml phorbol12-myristate 13-acetate (PMA) and 5 µg/ml phytohaemagglutinin (PHA) stimulation at24 h. TNFα could not be measured in the absence of PMA/PHAstimulation. * Mean value was significantly different compared with vehicle controls(P<0·05; one-way ANOVA with Dunnett’s posthoc test). No significant effects were observed following treatment withcaffeic acid, ferulic acid, isoferulic acid, 5-(3'-hydroxyphenyl) propionic acid,5-(3',4'-dihydroxyphenyl) propionic acid, 5-(3'-methoxy-4'-hydroxyphenyl) propionicacid, homoprotocatechuic acid, 3-(4' hydroxyphenyl) lactic acid, hippuric acid or4'-hydroxyhippuric acid. Examples of the data from which these heat maps are derivedare provided in the online Supplementary data to allow an assessment of thevariability observed in these studies.
Mentions: After treatment with colonic catabolites of polyphenols (phenolic acids) for 48 h, wemeasured the density of viable Jurkat CD4+ T-lymphocytes in culture and theconcentrations of the pro-inflammatory cytokines IL-2, IL-8 and TNFα inthe cell culture media. Comparisons between (poly)phenol treatments and vehicle controlsrevealed multiple effects on growth and cytokine release (Fig. 2). The low molecular weight (110–139 g/mol) catabolites catechol,phloroglucinol and 4-hydroxybenzoic acid caused significant declines in cell number(P<0·05; one-way ANOVA with Dunnett’s post hoctest) and significantly induced release of IL-2, IL-8 and TNFα(P<0·05; one-way ANOVA with Dunnett’s post hoctest), whereas tyrosol significantly decreased IL-2 and IL-8 release from non-stimulatedcells at 1 µmol/l (P<0·05; one-way ANOVA with Dunnett’spost hoc test). Mid-molecular weight catabolites (168–195 g/mol)reduced pro-inflammatory cytokine release, with significant reduction in IL-2 release fromnon-stimulated cells by 1 µmol/l 4'-hydroxymandelic acid and vanillic acid(P<0·05; one-way ANOVA with Dunnett’s post hoctest). Higher molecular weight test compounds (196–252 g/mol) showed mixed effects:feruloylglycine at 1 µmol/l significantly increased the release of IL-2 andTNFα, and isoferuloylglycine at 30 µmol/l significantly reduced IL-8release from PMA/PHA-stimulated Jurkat CD4+ T-lymphocytes(P<0·05; one-way ANOVA with Dunnett’s post hoctest).Fig. 2

Bottom Line: We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes.A number of (poly)phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release.These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of pro-inflammatory mediators in people at risk of chronic inflammation.

View Article: PubMed Central - PubMed

Affiliation: 1Department of Musculoskeletal Biology,Institute of Ageing and Chronic Disease,University of Liverpool,Liverpool L7 8TX,UK.

ABSTRACT
Diets rich in fruits and vegetables (FV), which contain (poly)phenols, protect against age-related inflammation and chronic diseases. T-lymphocytes contribute to systemic cytokine production and are modulated by FV intake. Little is known about the relative potency of different (poly)phenols in modulating cytokine release by lymphocytes. We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes. Test compounds were incubated with Jurkat cells for 48 h at 1 and 30 µm, with or without phorbol ester treatment at 24 h to induce cytokine release. Three test compounds that reduced cytokine release were further incubated with primary lymphocytes at 0·2 and 1 µm for 24 h, with lipopolysaccharide added at 5 h. Cytokine release was measured, and generation of H2O2 by test compounds was determined to assess any potential correlations with cytokine release. A number of (poly)phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release. Resveratrol, isorhamnetin, curcumin, vanillic acid and specific (poly)phenol mixtures reduced pro-inflammatory cytokine release from T-lymphocytes, and there was evidence for interaction between (poly)phenols to further modulate cytokine release. The release of interferon-γ induced protein 10 by primary lymphocytes was significantly reduced following treatment with 1 µm isorhamnetin (P<0·05). These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of pro-inflammatory mediators in people at risk of chronic inflammation.

No MeSH data available.


Related in: MedlinePlus