Limits...
Identification of (poly)phenol treatments that modulate the release of pro-inflammatory cytokines by human lymphocytes.

Ford CT, Richardson S, McArdle F, Lotito SB, Crozier A, McArdle A, Jackson MJ - Br. J. Nutr. (2016)

Bottom Line: We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes.A number of (poly)phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release.These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of pro-inflammatory mediators in people at risk of chronic inflammation.

View Article: PubMed Central - PubMed

Affiliation: 1Department of Musculoskeletal Biology,Institute of Ageing and Chronic Disease,University of Liverpool,Liverpool L7 8TX,UK.

ABSTRACT
Diets rich in fruits and vegetables (FV), which contain (poly)phenols, protect against age-related inflammation and chronic diseases. T-lymphocytes contribute to systemic cytokine production and are modulated by FV intake. Little is known about the relative potency of different (poly)phenols in modulating cytokine release by lymphocytes. We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes. Test compounds were incubated with Jurkat cells for 48 h at 1 and 30 µm, with or without phorbol ester treatment at 24 h to induce cytokine release. Three test compounds that reduced cytokine release were further incubated with primary lymphocytes at 0·2 and 1 µm for 24 h, with lipopolysaccharide added at 5 h. Cytokine release was measured, and generation of H2O2 by test compounds was determined to assess any potential correlations with cytokine release. A number of (poly)phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release. Resveratrol, isorhamnetin, curcumin, vanillic acid and specific (poly)phenol mixtures reduced pro-inflammatory cytokine release from T-lymphocytes, and there was evidence for interaction between (poly)phenols to further modulate cytokine release. The release of interferon-γ induced protein 10 by primary lymphocytes was significantly reduced following treatment with 1 µm isorhamnetin (P<0·05). These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of pro-inflammatory mediators in people at risk of chronic inflammation.

No MeSH data available.


Related in: MedlinePlus

Structures of (poly)phenols used in cell culture experiments. Confirmed metabolicrelationships are shown: , metabolite of quercetin(23,24); , metabolite of(–)-epigallocatechin-3-O-gallate(25,26); , metabolite ofcyanidin-3-O-glucoside(27); , metabolite ofpelargonidin-3-O-glucoside(28); and , metabolite of chlorogenic acid(29). The phenolic catabolite structures are approximately ordered fromthose produced in the small intestine (top) to those derived in the proximalgastrointestinal tract via colonic catabolism (bottom).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4836295&req=5

fig1: Structures of (poly)phenols used in cell culture experiments. Confirmed metabolicrelationships are shown: , metabolite of quercetin(23,24); , metabolite of(–)-epigallocatechin-3-O-gallate(25,26); , metabolite ofcyanidin-3-O-glucoside(27); , metabolite ofpelargonidin-3-O-glucoside(28); and , metabolite of chlorogenic acid(29). The phenolic catabolite structures are approximately ordered fromthose produced in the small intestine (top) to those derived in the proximalgastrointestinal tract via colonic catabolism (bottom).

Mentions: Reagents were purchased from Sigma-Aldrich unless stated otherwise. Sigma-Aldrich alsosupplied resveratrol, quercetin, isorhamnetin, 3-O-methylquercetin,curcumin, (–)-epigallocatechin-3-O-gallate,pelargonidin-3-O-glucoside, cyanidin-3-O-glucoside,chlorogenic acid (5-O-caffeoylquinic acid), punicalagin, phloroglucinol(1,3,5-trihydroxybenzene), pyrogallol (1,2,3-trihydroxybenzene), catechol(1,2-dihydroxybenzene), protocatechuic acid (3,4-dihydroxybenzoic acid), 4-hydroxybenzoicacid, homoprotocatechuic acid (3',4'-dihydroxyphenylacetic acid), vanillic acid(3-methoxy-4-hydroxybenzoic acid), homovanillic acid (3'-methoxy-4'-hydroxyphenylaceticacid), 4'-hydroxyphenylacetic acid, 4'-hydroxymandelic acid, 5-(3'-hydroxyphenyl)propionic acid, 3-(4'-hydroxyphenyl) lactic acid, caffeic acid, dihydrocaffeic acid(3-(3',4'-dihydroxyphenyl) propionic acid), ferulic acid, isoferulic acid, dihydroferulicacid (3-(3'-methoxy-4'-hydroxyphenyl) propionic acid), hippuric acid and tyrosol.4'-Hydroxyhippuric acid was obtained from Bachem Ltd. Feruloylglycine andisoferuloylglycine were generous gifts from Professor Takao Yokota (Teikyo University).Fig. 1 shows the structures of the compoundstested and highlights the metabolic relationships between the compounds.Fig. 1


Identification of (poly)phenol treatments that modulate the release of pro-inflammatory cytokines by human lymphocytes.

Ford CT, Richardson S, McArdle F, Lotito SB, Crozier A, McArdle A, Jackson MJ - Br. J. Nutr. (2016)

Structures of (poly)phenols used in cell culture experiments. Confirmed metabolicrelationships are shown: , metabolite of quercetin(23,24); , metabolite of(–)-epigallocatechin-3-O-gallate(25,26); , metabolite ofcyanidin-3-O-glucoside(27); , metabolite ofpelargonidin-3-O-glucoside(28); and , metabolite of chlorogenic acid(29). The phenolic catabolite structures are approximately ordered fromthose produced in the small intestine (top) to those derived in the proximalgastrointestinal tract via colonic catabolism (bottom).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836295&req=5

fig1: Structures of (poly)phenols used in cell culture experiments. Confirmed metabolicrelationships are shown: , metabolite of quercetin(23,24); , metabolite of(–)-epigallocatechin-3-O-gallate(25,26); , metabolite ofcyanidin-3-O-glucoside(27); , metabolite ofpelargonidin-3-O-glucoside(28); and , metabolite of chlorogenic acid(29). The phenolic catabolite structures are approximately ordered fromthose produced in the small intestine (top) to those derived in the proximalgastrointestinal tract via colonic catabolism (bottom).
Mentions: Reagents were purchased from Sigma-Aldrich unless stated otherwise. Sigma-Aldrich alsosupplied resveratrol, quercetin, isorhamnetin, 3-O-methylquercetin,curcumin, (–)-epigallocatechin-3-O-gallate,pelargonidin-3-O-glucoside, cyanidin-3-O-glucoside,chlorogenic acid (5-O-caffeoylquinic acid), punicalagin, phloroglucinol(1,3,5-trihydroxybenzene), pyrogallol (1,2,3-trihydroxybenzene), catechol(1,2-dihydroxybenzene), protocatechuic acid (3,4-dihydroxybenzoic acid), 4-hydroxybenzoicacid, homoprotocatechuic acid (3',4'-dihydroxyphenylacetic acid), vanillic acid(3-methoxy-4-hydroxybenzoic acid), homovanillic acid (3'-methoxy-4'-hydroxyphenylaceticacid), 4'-hydroxyphenylacetic acid, 4'-hydroxymandelic acid, 5-(3'-hydroxyphenyl)propionic acid, 3-(4'-hydroxyphenyl) lactic acid, caffeic acid, dihydrocaffeic acid(3-(3',4'-dihydroxyphenyl) propionic acid), ferulic acid, isoferulic acid, dihydroferulicacid (3-(3'-methoxy-4'-hydroxyphenyl) propionic acid), hippuric acid and tyrosol.4'-Hydroxyhippuric acid was obtained from Bachem Ltd. Feruloylglycine andisoferuloylglycine were generous gifts from Professor Takao Yokota (Teikyo University).Fig. 1 shows the structures of the compoundstested and highlights the metabolic relationships between the compounds.Fig. 1

Bottom Line: We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes.A number of (poly)phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release.These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of pro-inflammatory mediators in people at risk of chronic inflammation.

View Article: PubMed Central - PubMed

Affiliation: 1Department of Musculoskeletal Biology,Institute of Ageing and Chronic Disease,University of Liverpool,Liverpool L7 8TX,UK.

ABSTRACT
Diets rich in fruits and vegetables (FV), which contain (poly)phenols, protect against age-related inflammation and chronic diseases. T-lymphocytes contribute to systemic cytokine production and are modulated by FV intake. Little is known about the relative potency of different (poly)phenols in modulating cytokine release by lymphocytes. We compared thirty-one (poly)phenols and six (poly)phenol mixtures for effects on pro-inflammatory cytokine release by Jurkat T-lymphocytes. Test compounds were incubated with Jurkat cells for 48 h at 1 and 30 µm, with or without phorbol ester treatment at 24 h to induce cytokine release. Three test compounds that reduced cytokine release were further incubated with primary lymphocytes at 0·2 and 1 µm for 24 h, with lipopolysaccharide added at 5 h. Cytokine release was measured, and generation of H2O2 by test compounds was determined to assess any potential correlations with cytokine release. A number of (poly)phenols significantly altered cytokine release from Jurkat cells (P<0·05), but H2O2 generation did not correlate with cytokine release. Resveratrol, isorhamnetin, curcumin, vanillic acid and specific (poly)phenol mixtures reduced pro-inflammatory cytokine release from T-lymphocytes, and there was evidence for interaction between (poly)phenols to further modulate cytokine release. The release of interferon-γ induced protein 10 by primary lymphocytes was significantly reduced following treatment with 1 µm isorhamnetin (P<0·05). These results suggest that (poly)phenols derived from onions, turmeric, red grapes, green tea and açai berries may help reduce the release of pro-inflammatory mediators in people at risk of chronic inflammation.

No MeSH data available.


Related in: MedlinePlus