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Identification of biomarkers for endometriosis using clinical proteomics.

Zhao Y, Liu YN, Li Y, Tian L, Ye X, Cui H, Chang XH - Chin. Med. J. (2015)

Bottom Line: We found a cluster of 5 peptides (4210, 5264, 2660, 5635, and 5904 Da), using 3 peptides (4210, 5904, 2660 Da) to discriminate EM patients from healthy volunteers, with 96.67% sensitivity and 100% specificity.ClinProt can identify EM biomarkers, which - most notably - distinguish even early-stage or minimal disease.We found 5 stable peaks at 4210, 5264, 2660, 5635, and 5904 Da as potential EM biomarkers, the strongest of which were associated with ATP1B4 (4210 Da) and FGA (5904 Da); this indicates that ATP1B4 and FGA are associated with EM pathogenesis.

View Article: PubMed Central - PubMed

Affiliation: Gynecology Oncology Center, Peking University People's Hospital, Beijing 100044, China.

ABSTRACT

Background: We investigated possible biomarkers for endometriosis (EM) using the ClinProt technique and proteomics methods.

Methods: We enrolled 50 patients with EM, 34 with benign ovarian neoplasms and 40 healthy volunteers in this study. Serum proteomic spectra were generated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) combined with weak cationic exchange (WCX) magnetic beads. Possible biomarkers were analyzed by a random and repeat pattern model-validation method that we designed, and ClinProtools software, results were refined using online liquid chromatography-tandem MS.

Results: We found a cluster of 5 peptides (4210, 5264, 2660, 5635, and 5904 Da), using 3 peptides (4210, 5904, 2660 Da) to discriminate EM patients from healthy volunteers, with 96.67% sensitivity and 100% specificity. We selected 4210 and 5904 m/z, which differed most between patients with EM and controls, and identified them as fragments of ATP1B4, and the fibrinogen alpha (FGA) isoform 1/2 of the FGA chain precursor, respectively.

Conclusions: ClinProt can identify EM biomarkers, which - most notably - distinguish even early-stage or minimal disease. We found 5 stable peaks at 4210, 5264, 2660, 5635, and 5904 Da as potential EM biomarkers, the strongest of which were associated with ATP1B4 (4210 Da) and FGA (5904 Da); this indicates that ATP1B4 and FGA are associated with EM pathogenesis.

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Related in: MedlinePlus

Protein standard fingerprints in different experimental runs. (a) Six proteins in different experimental runs; (b) Entire standard fingerprint in different experimental runs. Blue: 1st run; red: 2nd run; green: 3rd run; purple: 4th run.
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Figure 1: Protein standard fingerprints in different experimental runs. (a) Six proteins in different experimental runs; (b) Entire standard fingerprint in different experimental runs. Blue: 1st run; red: 2nd run; green: 3rd run; purple: 4th run.

Mentions: Stabilization of the mass spectrum using different sample runs was the means of verifying reproducibility of the ClinProt System, so in this study we tested the system stabilization first. We also used commercial standard sample as a reference to compare different chips and experimental runs in which six typical proteins were detected; the results show that the mean coefficient of variation (CV) was < 10%, which indicates good system reproducibility [Figure 1] and indicates that our results are credible.


Identification of biomarkers for endometriosis using clinical proteomics.

Zhao Y, Liu YN, Li Y, Tian L, Ye X, Cui H, Chang XH - Chin. Med. J. (2015)

Protein standard fingerprints in different experimental runs. (a) Six proteins in different experimental runs; (b) Entire standard fingerprint in different experimental runs. Blue: 1st run; red: 2nd run; green: 3rd run; purple: 4th run.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836258&req=5

Figure 1: Protein standard fingerprints in different experimental runs. (a) Six proteins in different experimental runs; (b) Entire standard fingerprint in different experimental runs. Blue: 1st run; red: 2nd run; green: 3rd run; purple: 4th run.
Mentions: Stabilization of the mass spectrum using different sample runs was the means of verifying reproducibility of the ClinProt System, so in this study we tested the system stabilization first. We also used commercial standard sample as a reference to compare different chips and experimental runs in which six typical proteins were detected; the results show that the mean coefficient of variation (CV) was < 10%, which indicates good system reproducibility [Figure 1] and indicates that our results are credible.

Bottom Line: We found a cluster of 5 peptides (4210, 5264, 2660, 5635, and 5904 Da), using 3 peptides (4210, 5904, 2660 Da) to discriminate EM patients from healthy volunteers, with 96.67% sensitivity and 100% specificity.ClinProt can identify EM biomarkers, which - most notably - distinguish even early-stage or minimal disease.We found 5 stable peaks at 4210, 5264, 2660, 5635, and 5904 Da as potential EM biomarkers, the strongest of which were associated with ATP1B4 (4210 Da) and FGA (5904 Da); this indicates that ATP1B4 and FGA are associated with EM pathogenesis.

View Article: PubMed Central - PubMed

Affiliation: Gynecology Oncology Center, Peking University People's Hospital, Beijing 100044, China.

ABSTRACT

Background: We investigated possible biomarkers for endometriosis (EM) using the ClinProt technique and proteomics methods.

Methods: We enrolled 50 patients with EM, 34 with benign ovarian neoplasms and 40 healthy volunteers in this study. Serum proteomic spectra were generated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MS) combined with weak cationic exchange (WCX) magnetic beads. Possible biomarkers were analyzed by a random and repeat pattern model-validation method that we designed, and ClinProtools software, results were refined using online liquid chromatography-tandem MS.

Results: We found a cluster of 5 peptides (4210, 5264, 2660, 5635, and 5904 Da), using 3 peptides (4210, 5904, 2660 Da) to discriminate EM patients from healthy volunteers, with 96.67% sensitivity and 100% specificity. We selected 4210 and 5904 m/z, which differed most between patients with EM and controls, and identified them as fragments of ATP1B4, and the fibrinogen alpha (FGA) isoform 1/2 of the FGA chain precursor, respectively.

Conclusions: ClinProt can identify EM biomarkers, which - most notably - distinguish even early-stage or minimal disease. We found 5 stable peaks at 4210, 5264, 2660, 5635, and 5904 Da as potential EM biomarkers, the strongest of which were associated with ATP1B4 (4210 Da) and FGA (5904 Da); this indicates that ATP1B4 and FGA are associated with EM pathogenesis.

Show MeSH
Related in: MedlinePlus