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Clinicopathological study of sporadic Burkitt lymphoma in children.

Huang H, Liu ZL, Zeng H, Zhang SH, Huang CS, Xu HY, Wu Y, Zeng ST, Xiong F, Yang WP - Chin. Med. J. (2015)

Bottom Line: Polypoid BL showed a lower clinical stage (P = 0.002), and advanced clinical stage and low serum albumin level at diagnosis were associated with poor outcome (P = 0.024 and 0.053, respectively).The anatomical location, growth pattern and serum albumin level of BL were associated with biological behavior.MUM1 may be a potential adverse prognostic marker, and not associated with EBV infection status.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Jiangxi Children's Hospital, Nanchang, Jiangxi 330006, China.

ABSTRACT

Background: Non-Hodgkin lymphoma is the fourth most common malignant tumors in children, Burkitt lymphoma (BL) accounts for 30-50% of all pediatric lymphomas. The aim of this study was to investigate the clinicopathologic features, immunophenotype, Epstein-Barr virus (EBV) infection and c-myc gene rearrangement of sporadic BL in children.

Methods: Ninety-two cases of pediatric BL were retrospectively analyzed for clinical features, immunohistochemistry, EBV-encoded RNA (EBER) status by in situ hybridization and c-myc gene rearrangement by fluorescence in situ hybridization.

Results: In the 92 cases, male is predominant in sex distribution (M: F = 3.38:1). The average age at diagnosis was 4.97 years. Polypoid BL showed a lower clinical stage (P = 0.002), and advanced clinical stage and low serum albumin level at diagnosis were associated with poor outcome (P = 0.024 and 0.053, respectively). The positive expression of CDl0, B-cell lymphoma-6, MUMl and EBER were 95.7% (88 cases), 92.4% (85 cases), 22.8% (21 cases), 41.3% (38 cases), respectively. The expression of MUM1 were not associated with EBV infection status (P = 1.000). c-myc gene rearrangement was detected in 94.6% (87/92). Clinical treatment information for 54 cases was collected, 21 patients died of tumor after surgery alone, 33 patients received surgery and chemotherapy, and of which six patients died shortly afterwords (MUM1 positive expression in 3 cases, P = 0.076).

Conclusions: The anatomical location, growth pattern and serum albumin level of BL were associated with biological behavior. MUM1 may be a potential adverse prognostic marker, and not associated with EBV infection status.

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Related in: MedlinePlus

(a) Tumor cells were positive for CD20 (original magnification ×200); (b) Tumor cells were positive for B-cell lymphoma-6 (original magnification ×200); (c) In situ hybridization for Epstein-Barr virus-encode RNA was positive (original magnification ×200); (d) Tumor cells were positive for Ki-67 (original magnification ×200).
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Figure 3: (a) Tumor cells were positive for CD20 (original magnification ×200); (b) Tumor cells were positive for B-cell lymphoma-6 (original magnification ×200); (c) In situ hybridization for Epstein-Barr virus-encode RNA was positive (original magnification ×200); (d) Tumor cells were positive for Ki-67 (original magnification ×200).

Mentions: All of the cases were positive for CD20 [Figure 3a] and CD79a and negative for BCL-2, CD3, and TdT. The positive expression rate of CDl0, BCL-6 [Figure 3b], MUMl and EBER [Figure 3c] were 95.7% (88 cases), 92.4% (85 cases), 22.8% (21 cases), 41.3% (38 cases), respectively, Ki-67 + ≥90% [Figure 3d]. According to the expression of MUM1 and BCL-6, BL were divided into GC group 65 cases, late-GC group 20 cases, post-GC Group 1 case and mantle cell-like group 6 cases. The expression of MUM1 was not associated with EBV infection status (P = 1.000).


Clinicopathological study of sporadic Burkitt lymphoma in children.

Huang H, Liu ZL, Zeng H, Zhang SH, Huang CS, Xu HY, Wu Y, Zeng ST, Xiong F, Yang WP - Chin. Med. J. (2015)

(a) Tumor cells were positive for CD20 (original magnification ×200); (b) Tumor cells were positive for B-cell lymphoma-6 (original magnification ×200); (c) In situ hybridization for Epstein-Barr virus-encode RNA was positive (original magnification ×200); (d) Tumor cells were positive for Ki-67 (original magnification ×200).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836256&req=5

Figure 3: (a) Tumor cells were positive for CD20 (original magnification ×200); (b) Tumor cells were positive for B-cell lymphoma-6 (original magnification ×200); (c) In situ hybridization for Epstein-Barr virus-encode RNA was positive (original magnification ×200); (d) Tumor cells were positive for Ki-67 (original magnification ×200).
Mentions: All of the cases were positive for CD20 [Figure 3a] and CD79a and negative for BCL-2, CD3, and TdT. The positive expression rate of CDl0, BCL-6 [Figure 3b], MUMl and EBER [Figure 3c] were 95.7% (88 cases), 92.4% (85 cases), 22.8% (21 cases), 41.3% (38 cases), respectively, Ki-67 + ≥90% [Figure 3d]. According to the expression of MUM1 and BCL-6, BL were divided into GC group 65 cases, late-GC group 20 cases, post-GC Group 1 case and mantle cell-like group 6 cases. The expression of MUM1 was not associated with EBV infection status (P = 1.000).

Bottom Line: Polypoid BL showed a lower clinical stage (P = 0.002), and advanced clinical stage and low serum albumin level at diagnosis were associated with poor outcome (P = 0.024 and 0.053, respectively).The anatomical location, growth pattern and serum albumin level of BL were associated with biological behavior.MUM1 may be a potential adverse prognostic marker, and not associated with EBV infection status.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Jiangxi Children's Hospital, Nanchang, Jiangxi 330006, China.

ABSTRACT

Background: Non-Hodgkin lymphoma is the fourth most common malignant tumors in children, Burkitt lymphoma (BL) accounts for 30-50% of all pediatric lymphomas. The aim of this study was to investigate the clinicopathologic features, immunophenotype, Epstein-Barr virus (EBV) infection and c-myc gene rearrangement of sporadic BL in children.

Methods: Ninety-two cases of pediatric BL were retrospectively analyzed for clinical features, immunohistochemistry, EBV-encoded RNA (EBER) status by in situ hybridization and c-myc gene rearrangement by fluorescence in situ hybridization.

Results: In the 92 cases, male is predominant in sex distribution (M: F = 3.38:1). The average age at diagnosis was 4.97 years. Polypoid BL showed a lower clinical stage (P = 0.002), and advanced clinical stage and low serum albumin level at diagnosis were associated with poor outcome (P = 0.024 and 0.053, respectively). The positive expression of CDl0, B-cell lymphoma-6, MUMl and EBER were 95.7% (88 cases), 92.4% (85 cases), 22.8% (21 cases), 41.3% (38 cases), respectively. The expression of MUM1 were not associated with EBV infection status (P = 1.000). c-myc gene rearrangement was detected in 94.6% (87/92). Clinical treatment information for 54 cases was collected, 21 patients died of tumor after surgery alone, 33 patients received surgery and chemotherapy, and of which six patients died shortly afterwords (MUM1 positive expression in 3 cases, P = 0.076).

Conclusions: The anatomical location, growth pattern and serum albumin level of BL were associated with biological behavior. MUM1 may be a potential adverse prognostic marker, and not associated with EBV infection status.

Show MeSH
Related in: MedlinePlus