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Genome-Wide Identification and Function Analyses of Heat Shock Transcription Factors in Potato.

Tang R, Zhu W, Song X, Lin X, Cai J, Wang M, Yang Q - Front Plant Sci (2016)

Bottom Line: Expression profiles of StHsfs in 12 different organs and tissues uncovered distinct spatial expression patterns of these genes and their potential roles in the process of growth and development.StHsf004, StHsf007, StHsf009, StHsf014, and StHsf019 were constitutively expressed under non-stress conditions, and some specific Hsfs became the predominant Hsfs in response to different abiotic stresses, indicating their important and diverse regulatory roles in adverse conditions.A co-expression network between StHsfs and StHsf -co-expressed genes was generated based on the publicly-available potato transcriptomic databases and identified key candidate StHsfs for further functional studies.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University Nanjing, China.

ABSTRACT
Heat shock transcription factors (Hsfs) play vital roles in the regulation of tolerance to various stresses in living organisms. To dissect the mechanisms of the Hsfs in potato adaptation to abiotic stresses, genome and transcriptome analyses of Hsf gene family were investigated in Solanum tuberosum L. Twenty-seven StHsf members were identified by bioinformatics and phylogenetic analyses and were classified into A, B, and C groups according to their structural and phylogenetic features. StHsfs in the same class shared similar gene structures and conserved motifs. The chromosomal location analysis showed that 27 Hsfs were located in 10 of 12 chromosomes (except chromosome 1 and chromosome 5) and that 18 of these genes formed 9 paralogous pairs. Expression profiles of StHsfs in 12 different organs and tissues uncovered distinct spatial expression patterns of these genes and their potential roles in the process of growth and development. Promoter and quantitative real-time polymerase chain reaction (qRT-PCR) detections of StHsfs were conducted and demonstrated that these genes were all responsive to various stresses. StHsf004, StHsf007, StHsf009, StHsf014, and StHsf019 were constitutively expressed under non-stress conditions, and some specific Hsfs became the predominant Hsfs in response to different abiotic stresses, indicating their important and diverse regulatory roles in adverse conditions. A co-expression network between StHsfs and StHsf -co-expressed genes was generated based on the publicly-available potato transcriptomic databases and identified key candidate StHsfs for further functional studies.

No MeSH data available.


Related in: MedlinePlus

Relative mRNA abundance of StHsf members in response to heat (A), drought (B) and cold stress (C) in the leaves.
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Figure 6: Relative mRNA abundance of StHsf members in response to heat (A), drought (B) and cold stress (C) in the leaves.

Mentions: To validate this hypothesis, qRT-PCR was used to determine the expression profiles of StHsf001, 004, 005, 007, 008, 009, 012, 014, 015, 016, 017, 018, 019, 021, 022, 024, 026, and 027 genes in leaves of potato plants subjected to heat, drought and cold stresses (Figure 6). These StHsf genes were selected from all subgroups and the primers were presented in Supplementary Table S1. The heat, drought and cold responsiveness of these genes was examined in the leaves of 1-month-old plants with short- (2 h and 6 h) and long-term (24 h) stress treatments. In order to clarify which genes play the major role during different stress conditions as a whole, the expression of StHsf001 at 0 h under the corresponding stress was set to 1 and the expression of other genes in different stages of treatment were compared with that. Generally, the expression levels of StHsf004, StHsf005, StHsf007, StHsf009, and StHsf014 were all higher than other genes during different stresses. Specifically, StHsf004, StHsf005, and StHsf009 became predominant StHsf transcripts during heat stress, especially StHsf004 and StHsf005 whose expression levels were approximately 200~300 times higher than that of the other members (Figure 6A); while StHsf004, StHsf007, StHsf009, and StHsf014 all played a leading role under drought stress (Figure 6B) and cold stress (Figure 6C). In addition, constitutive expression of StHsf004, StHsf005, StHsf007, and StHsf012 was observed before stress treatments, which is in accordance with the expression heatmap of the StHsf genes in potato leaves (Figure 4).


Genome-Wide Identification and Function Analyses of Heat Shock Transcription Factors in Potato.

Tang R, Zhu W, Song X, Lin X, Cai J, Wang M, Yang Q - Front Plant Sci (2016)

Relative mRNA abundance of StHsf members in response to heat (A), drought (B) and cold stress (C) in the leaves.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836240&req=5

Figure 6: Relative mRNA abundance of StHsf members in response to heat (A), drought (B) and cold stress (C) in the leaves.
Mentions: To validate this hypothesis, qRT-PCR was used to determine the expression profiles of StHsf001, 004, 005, 007, 008, 009, 012, 014, 015, 016, 017, 018, 019, 021, 022, 024, 026, and 027 genes in leaves of potato plants subjected to heat, drought and cold stresses (Figure 6). These StHsf genes were selected from all subgroups and the primers were presented in Supplementary Table S1. The heat, drought and cold responsiveness of these genes was examined in the leaves of 1-month-old plants with short- (2 h and 6 h) and long-term (24 h) stress treatments. In order to clarify which genes play the major role during different stress conditions as a whole, the expression of StHsf001 at 0 h under the corresponding stress was set to 1 and the expression of other genes in different stages of treatment were compared with that. Generally, the expression levels of StHsf004, StHsf005, StHsf007, StHsf009, and StHsf014 were all higher than other genes during different stresses. Specifically, StHsf004, StHsf005, and StHsf009 became predominant StHsf transcripts during heat stress, especially StHsf004 and StHsf005 whose expression levels were approximately 200~300 times higher than that of the other members (Figure 6A); while StHsf004, StHsf007, StHsf009, and StHsf014 all played a leading role under drought stress (Figure 6B) and cold stress (Figure 6C). In addition, constitutive expression of StHsf004, StHsf005, StHsf007, and StHsf012 was observed before stress treatments, which is in accordance with the expression heatmap of the StHsf genes in potato leaves (Figure 4).

Bottom Line: Expression profiles of StHsfs in 12 different organs and tissues uncovered distinct spatial expression patterns of these genes and their potential roles in the process of growth and development.StHsf004, StHsf007, StHsf009, StHsf014, and StHsf019 were constitutively expressed under non-stress conditions, and some specific Hsfs became the predominant Hsfs in response to different abiotic stresses, indicating their important and diverse regulatory roles in adverse conditions.A co-expression network between StHsfs and StHsf -co-expressed genes was generated based on the publicly-available potato transcriptomic databases and identified key candidate StHsfs for further functional studies.

View Article: PubMed Central - PubMed

Affiliation: Biochemistry and Molecular Biology, College of Life Sciences, Nanjing Agricultural University Nanjing, China.

ABSTRACT
Heat shock transcription factors (Hsfs) play vital roles in the regulation of tolerance to various stresses in living organisms. To dissect the mechanisms of the Hsfs in potato adaptation to abiotic stresses, genome and transcriptome analyses of Hsf gene family were investigated in Solanum tuberosum L. Twenty-seven StHsf members were identified by bioinformatics and phylogenetic analyses and were classified into A, B, and C groups according to their structural and phylogenetic features. StHsfs in the same class shared similar gene structures and conserved motifs. The chromosomal location analysis showed that 27 Hsfs were located in 10 of 12 chromosomes (except chromosome 1 and chromosome 5) and that 18 of these genes formed 9 paralogous pairs. Expression profiles of StHsfs in 12 different organs and tissues uncovered distinct spatial expression patterns of these genes and their potential roles in the process of growth and development. Promoter and quantitative real-time polymerase chain reaction (qRT-PCR) detections of StHsfs were conducted and demonstrated that these genes were all responsive to various stresses. StHsf004, StHsf007, StHsf009, StHsf014, and StHsf019 were constitutively expressed under non-stress conditions, and some specific Hsfs became the predominant Hsfs in response to different abiotic stresses, indicating their important and diverse regulatory roles in adverse conditions. A co-expression network between StHsfs and StHsf -co-expressed genes was generated based on the publicly-available potato transcriptomic databases and identified key candidate StHsfs for further functional studies.

No MeSH data available.


Related in: MedlinePlus