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Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina.

Chen GE, Hitchcock A, Jackson PJ, Chaudhuri RR, Dickman MJ, Hunter CN, Canniffe DP - J. Bacteriol. (2016)

Bottom Line: Two unrelated classes of 8-vinyl reductase involved in the biosynthesis of chlorophylls are known to exist, BciA and BciB.Potential reasons for the presence of two 8-vinyl reductases in this strain, which is unique for cyanobacteria, are discussed.Carrying a reduced C-8 group may be of particular importance to organisms containing chlorophyll d Plant genomes also contain orthologs of both of these genes; thus, the bacterial progenitor of the chloroplast may also have contained both bciA and bciB.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom.

No MeSH data available.


Related in: MedlinePlus

Phylogenetic relationships among 8VR protein sequences compared with parent organism 16S rRNA phylogenies. Maximum-likelihood phylogenies of BciA (A) and BciB (B) homologs, compared with 16S rRNA phylogenies of the same organisms, are shown. The BciA and BciB trees were constructed from amino acid alignments using the PROTGAMMAAUTO model in RAxML version 8.2.4. The rRNA trees were constructed from nucleotide alignments using the GTRCAT model. The numbers on branches indicate the percent bootstrap support from 100 replicates, and the scale bars indicate the specified number of amino acid or nucleotide substitutions per site. Example organisms from cyanobacteria (cyan), purple nonsulfur bacteria (purple), green sulfur bacteria (green), green filamentous bacteria (amber), and Acidobacteria (red) are included.
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Figure 5: Phylogenetic relationships among 8VR protein sequences compared with parent organism 16S rRNA phylogenies. Maximum-likelihood phylogenies of BciA (A) and BciB (B) homologs, compared with 16S rRNA phylogenies of the same organisms, are shown. The BciA and BciB trees were constructed from amino acid alignments using the PROTGAMMAAUTO model in RAxML version 8.2.4. The rRNA trees were constructed from nucleotide alignments using the GTRCAT model. The numbers on branches indicate the percent bootstrap support from 100 replicates, and the scale bars indicate the specified number of amino acid or nucleotide substitutions per site. Example organisms from cyanobacteria (cyan), purple nonsulfur bacteria (purple), green sulfur bacteria (green), green filamentous bacteria (amber), and Acidobacteria (red) are included.

Mentions: Comparisons of the phylogenies obtained by maximum-likelihood analysis of BciA and BciB amino acid alignments with those obtained by analysis of 16S rRNA alignments from the same species are shown in Fig. 5A and B, respectively. The phylogenetic positions of A. marina BciA and BciB are both broadly consistent with those shown for A. marina in the 16S rRNA trees, suggesting that the bciA and bciB genes have not been acquired by horizontal transfer. However, the positions of Synechococcus spp. in the BciA tree and the clade containing the green sulfur bacteria in the BciB tree are inconsistent with the 16S rRNA phylogeny, indicating that there may have been lateral transfer events during the evolution of both bciA and bciB.


Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina.

Chen GE, Hitchcock A, Jackson PJ, Chaudhuri RR, Dickman MJ, Hunter CN, Canniffe DP - J. Bacteriol. (2016)

Phylogenetic relationships among 8VR protein sequences compared with parent organism 16S rRNA phylogenies. Maximum-likelihood phylogenies of BciA (A) and BciB (B) homologs, compared with 16S rRNA phylogenies of the same organisms, are shown. The BciA and BciB trees were constructed from amino acid alignments using the PROTGAMMAAUTO model in RAxML version 8.2.4. The rRNA trees were constructed from nucleotide alignments using the GTRCAT model. The numbers on branches indicate the percent bootstrap support from 100 replicates, and the scale bars indicate the specified number of amino acid or nucleotide substitutions per site. Example organisms from cyanobacteria (cyan), purple nonsulfur bacteria (purple), green sulfur bacteria (green), green filamentous bacteria (amber), and Acidobacteria (red) are included.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836224&req=5

Figure 5: Phylogenetic relationships among 8VR protein sequences compared with parent organism 16S rRNA phylogenies. Maximum-likelihood phylogenies of BciA (A) and BciB (B) homologs, compared with 16S rRNA phylogenies of the same organisms, are shown. The BciA and BciB trees were constructed from amino acid alignments using the PROTGAMMAAUTO model in RAxML version 8.2.4. The rRNA trees were constructed from nucleotide alignments using the GTRCAT model. The numbers on branches indicate the percent bootstrap support from 100 replicates, and the scale bars indicate the specified number of amino acid or nucleotide substitutions per site. Example organisms from cyanobacteria (cyan), purple nonsulfur bacteria (purple), green sulfur bacteria (green), green filamentous bacteria (amber), and Acidobacteria (red) are included.
Mentions: Comparisons of the phylogenies obtained by maximum-likelihood analysis of BciA and BciB amino acid alignments with those obtained by analysis of 16S rRNA alignments from the same species are shown in Fig. 5A and B, respectively. The phylogenetic positions of A. marina BciA and BciB are both broadly consistent with those shown for A. marina in the 16S rRNA trees, suggesting that the bciA and bciB genes have not been acquired by horizontal transfer. However, the positions of Synechococcus spp. in the BciA tree and the clade containing the green sulfur bacteria in the BciB tree are inconsistent with the 16S rRNA phylogeny, indicating that there may have been lateral transfer events during the evolution of both bciA and bciB.

Bottom Line: Two unrelated classes of 8-vinyl reductase involved in the biosynthesis of chlorophylls are known to exist, BciA and BciB.Potential reasons for the presence of two 8-vinyl reductases in this strain, which is unique for cyanobacteria, are discussed.Carrying a reduced C-8 group may be of particular importance to organisms containing chlorophyll d Plant genomes also contain orthologs of both of these genes; thus, the bacterial progenitor of the chloroplast may also have contained both bciA and bciB.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom.

No MeSH data available.


Related in: MedlinePlus