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Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina.

Chen GE, Hitchcock A, Jackson PJ, Chaudhuri RR, Dickman MJ, Hunter CN, Canniffe DP - J. Bacteriol. (2016)

Bottom Line: Two unrelated classes of 8-vinyl reductase involved in the biosynthesis of chlorophylls are known to exist, BciA and BciB.Potential reasons for the presence of two 8-vinyl reductases in this strain, which is unique for cyanobacteria, are discussed.Carrying a reduced C-8 group may be of particular importance to organisms containing chlorophyll d Plant genomes also contain orthologs of both of these genes; thus, the bacterial progenitor of the chloroplast may also have contained both bciA and bciB.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom.

No MeSH data available.


Related in: MedlinePlus

Growth and pigment analysis of described strains of Synechocystis. (A) Strains tested for growth under high light intensity on solid medium. (B) HPLC elution profiles and absorption spectra (inset) of Chls extracted from strains grown under moderate light intensity. Retention times of 20.3 and 19.9 min and Soret absorption maxima at 431 and 442 nm are indicative of 8E-Chl a and 8V-Chl a, respectively, in the HPLC solvents.
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Figure 3: Growth and pigment analysis of described strains of Synechocystis. (A) Strains tested for growth under high light intensity on solid medium. (B) HPLC elution profiles and absorption spectra (inset) of Chls extracted from strains grown under moderate light intensity. Retention times of 20.3 and 19.9 min and Soret absorption maxima at 431 and 442 nm are indicative of 8E-Chl a and 8V-Chl a, respectively, in the HPLC solvents.

Mentions: The strains expressing A. marina genes, along with the WT and ΔbciB strains, were tested for their ability to grow under high light. Patches of cells were incubated under constant illumination on solid medium. As expected, the ΔbciB strain was unable to grow under high light, consistent with previously published results (11, 13, 14), while complementation with both nmrA and frhB restored the ability of the ΔbciB strain to grow under these conditions, comparable to the growth of the WT (Fig. 3A).


Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina.

Chen GE, Hitchcock A, Jackson PJ, Chaudhuri RR, Dickman MJ, Hunter CN, Canniffe DP - J. Bacteriol. (2016)

Growth and pigment analysis of described strains of Synechocystis. (A) Strains tested for growth under high light intensity on solid medium. (B) HPLC elution profiles and absorption spectra (inset) of Chls extracted from strains grown under moderate light intensity. Retention times of 20.3 and 19.9 min and Soret absorption maxima at 431 and 442 nm are indicative of 8E-Chl a and 8V-Chl a, respectively, in the HPLC solvents.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836224&req=5

Figure 3: Growth and pigment analysis of described strains of Synechocystis. (A) Strains tested for growth under high light intensity on solid medium. (B) HPLC elution profiles and absorption spectra (inset) of Chls extracted from strains grown under moderate light intensity. Retention times of 20.3 and 19.9 min and Soret absorption maxima at 431 and 442 nm are indicative of 8E-Chl a and 8V-Chl a, respectively, in the HPLC solvents.
Mentions: The strains expressing A. marina genes, along with the WT and ΔbciB strains, were tested for their ability to grow under high light. Patches of cells were incubated under constant illumination on solid medium. As expected, the ΔbciB strain was unable to grow under high light, consistent with previously published results (11, 13, 14), while complementation with both nmrA and frhB restored the ability of the ΔbciB strain to grow under these conditions, comparable to the growth of the WT (Fig. 3A).

Bottom Line: Two unrelated classes of 8-vinyl reductase involved in the biosynthesis of chlorophylls are known to exist, BciA and BciB.Potential reasons for the presence of two 8-vinyl reductases in this strain, which is unique for cyanobacteria, are discussed.Carrying a reduced C-8 group may be of particular importance to organisms containing chlorophyll d Plant genomes also contain orthologs of both of these genes; thus, the bacterial progenitor of the chloroplast may also have contained both bciA and bciB.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom.

No MeSH data available.


Related in: MedlinePlus