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Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina.

Chen GE, Hitchcock A, Jackson PJ, Chaudhuri RR, Dickman MJ, Hunter CN, Canniffe DP - J. Bacteriol. (2016)

Bottom Line: Two unrelated classes of 8-vinyl reductase involved in the biosynthesis of chlorophylls are known to exist, BciA and BciB.Potential reasons for the presence of two 8-vinyl reductases in this strain, which is unique for cyanobacteria, are discussed.Carrying a reduced C-8 group may be of particular importance to organisms containing chlorophyll d Plant genomes also contain orthologs of both of these genes; thus, the bacterial progenitor of the chloroplast may also have contained both bciA and bciB.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom.

No MeSH data available.


Related in: MedlinePlus

The terminal steps in the biosynthesis of Chls a and d. (A) The precursor 8V-Chlide (IUPAC numbered) is reduced to Chlide by an 8VR prior to the addition of phytol to the C-17 propionate side chain by Chl synthase (ChlG). In A. marina, the currently unidentified Chl d synthase oxidizes the C-3 vinyl group of Chl a to a characteristic formyl group. (B) The Chl d synthase-catalyzed oxidation results in a red shift in the Qy absorption maximum of the pigment from 665 nm to 697 nm (in methanol).
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Figure 1: The terminal steps in the biosynthesis of Chls a and d. (A) The precursor 8V-Chlide (IUPAC numbered) is reduced to Chlide by an 8VR prior to the addition of phytol to the C-17 propionate side chain by Chl synthase (ChlG). In A. marina, the currently unidentified Chl d synthase oxidizes the C-3 vinyl group of Chl a to a characteristic formyl group. (B) The Chl d synthase-catalyzed oxidation results in a red shift in the Qy absorption maximum of the pigment from 665 nm to 697 nm (in methanol).

Mentions: With the exception of the marine cyanobacterial Prochlorococcus spp. (4), the majority of Chls used by oxygenic phototrophs carry an ethyl group at the C-8 position (8E), the product of an 8-vinyl reductase (8VR) acting on a biosynthetic precursor, 8-vinyl (8V) chlorophyllide (Chlide) (5) (Fig. 1A). Two unrelated classes of 8VR are known to exist in oxygenic phototrophs, BciA and BciB.


Two Unrelated 8-Vinyl Reductases Ensure Production of Mature Chlorophylls in Acaryochloris marina.

Chen GE, Hitchcock A, Jackson PJ, Chaudhuri RR, Dickman MJ, Hunter CN, Canniffe DP - J. Bacteriol. (2016)

The terminal steps in the biosynthesis of Chls a and d. (A) The precursor 8V-Chlide (IUPAC numbered) is reduced to Chlide by an 8VR prior to the addition of phytol to the C-17 propionate side chain by Chl synthase (ChlG). In A. marina, the currently unidentified Chl d synthase oxidizes the C-3 vinyl group of Chl a to a characteristic formyl group. (B) The Chl d synthase-catalyzed oxidation results in a red shift in the Qy absorption maximum of the pigment from 665 nm to 697 nm (in methanol).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836224&req=5

Figure 1: The terminal steps in the biosynthesis of Chls a and d. (A) The precursor 8V-Chlide (IUPAC numbered) is reduced to Chlide by an 8VR prior to the addition of phytol to the C-17 propionate side chain by Chl synthase (ChlG). In A. marina, the currently unidentified Chl d synthase oxidizes the C-3 vinyl group of Chl a to a characteristic formyl group. (B) The Chl d synthase-catalyzed oxidation results in a red shift in the Qy absorption maximum of the pigment from 665 nm to 697 nm (in methanol).
Mentions: With the exception of the marine cyanobacterial Prochlorococcus spp. (4), the majority of Chls used by oxygenic phototrophs carry an ethyl group at the C-8 position (8E), the product of an 8-vinyl reductase (8VR) acting on a biosynthetic precursor, 8-vinyl (8V) chlorophyllide (Chlide) (5) (Fig. 1A). Two unrelated classes of 8VR are known to exist in oxygenic phototrophs, BciA and BciB.

Bottom Line: Two unrelated classes of 8-vinyl reductase involved in the biosynthesis of chlorophylls are known to exist, BciA and BciB.Potential reasons for the presence of two 8-vinyl reductases in this strain, which is unique for cyanobacteria, are discussed.Carrying a reduced C-8 group may be of particular importance to organisms containing chlorophyll d Plant genomes also contain orthologs of both of these genes; thus, the bacterial progenitor of the chloroplast may also have contained both bciA and bciB.

View Article: PubMed Central - PubMed

Affiliation: Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom.

No MeSH data available.


Related in: MedlinePlus