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Enantioselective Utilization of D-Amino Acids by Deep-Sea Microorganisms.

Kubota T, Kobayashi T, Nunoura T, Maruyama F, Deguchi S - Front Microbiol (2016)

Bottom Line: Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments.In contrast, the Nautella strains isolated from shallow-sea grew only with L-Val.No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology Yokosuka, Japan.

ABSTRACT
Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments. The isolates were phylogenetically assigned to Alphaproteobacteria, Gammmaproteobacteria, and Bacilli. Some of the isolates exhibited high enantioselective degradation activities to various DAAs. In particular, the Alphaproteobacteria Nautella sp. strain A04V exhibited robust growth in minimal medium supplemented with D-Val as a sole carbon and nitrogen source, whereas its growth was poor on minimal medium supplemented with L-Val instead of D-Val. Its growth was facilitated most when racemic mixtures of valine were used. In contrast, the Nautella strains isolated from shallow-sea grew only with L-Val. No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.

No MeSH data available.


Related in: MedlinePlus

Effects of valine concentrations in the medium on the growth of Nautella sp. strain A04V. The substrates used for cultivation were: 6 mM D-Val (A), 6 mM racemic Val at D:L = 5:1 mM (B), D:L = 4:2 mM (C), D:L = 3:3 mM (D), D:L = 2:4 mM (E), D:L = 1:5 mM (F), and 6 mM L-Val (G) as the sole carbon and nitrogen sources. The concentrations of D-Val (filled circles) and L-Val (open circles) were determined by HPLC. The growth of the strain was measured instrumentally by determining the optical density at 660 nm (OD660) (open triangles).
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Figure 6: Effects of valine concentrations in the medium on the growth of Nautella sp. strain A04V. The substrates used for cultivation were: 6 mM D-Val (A), 6 mM racemic Val at D:L = 5:1 mM (B), D:L = 4:2 mM (C), D:L = 3:3 mM (D), D:L = 2:4 mM (E), D:L = 1:5 mM (F), and 6 mM L-Val (G) as the sole carbon and nitrogen sources. The concentrations of D-Val (filled circles) and L-Val (open circles) were determined by HPLC. The growth of the strain was measured instrumentally by determining the optical density at 660 nm (OD660) (open triangles).

Mentions: The rate of consumption of Val (D-, L-, and D, L-Val) during the growth of strain A04V was determined by HPLC (Figure 6). D-, L-, and D, L-Val were completely depleted from the medium in the early stationary phase. Intriguingly, D-Val was consumed faster than L-Val during cultivation when the L- or D-form was supplemented as sole carbon and nitrogen sources, whereas L-Val and D-Val were consumed at the same rate when racemic mixtures were used (Figure 6).


Enantioselective Utilization of D-Amino Acids by Deep-Sea Microorganisms.

Kubota T, Kobayashi T, Nunoura T, Maruyama F, Deguchi S - Front Microbiol (2016)

Effects of valine concentrations in the medium on the growth of Nautella sp. strain A04V. The substrates used for cultivation were: 6 mM D-Val (A), 6 mM racemic Val at D:L = 5:1 mM (B), D:L = 4:2 mM (C), D:L = 3:3 mM (D), D:L = 2:4 mM (E), D:L = 1:5 mM (F), and 6 mM L-Val (G) as the sole carbon and nitrogen sources. The concentrations of D-Val (filled circles) and L-Val (open circles) were determined by HPLC. The growth of the strain was measured instrumentally by determining the optical density at 660 nm (OD660) (open triangles).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836201&req=5

Figure 6: Effects of valine concentrations in the medium on the growth of Nautella sp. strain A04V. The substrates used for cultivation were: 6 mM D-Val (A), 6 mM racemic Val at D:L = 5:1 mM (B), D:L = 4:2 mM (C), D:L = 3:3 mM (D), D:L = 2:4 mM (E), D:L = 1:5 mM (F), and 6 mM L-Val (G) as the sole carbon and nitrogen sources. The concentrations of D-Val (filled circles) and L-Val (open circles) were determined by HPLC. The growth of the strain was measured instrumentally by determining the optical density at 660 nm (OD660) (open triangles).
Mentions: The rate of consumption of Val (D-, L-, and D, L-Val) during the growth of strain A04V was determined by HPLC (Figure 6). D-, L-, and D, L-Val were completely depleted from the medium in the early stationary phase. Intriguingly, D-Val was consumed faster than L-Val during cultivation when the L- or D-form was supplemented as sole carbon and nitrogen sources, whereas L-Val and D-Val were consumed at the same rate when racemic mixtures were used (Figure 6).

Bottom Line: Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments.In contrast, the Nautella strains isolated from shallow-sea grew only with L-Val.No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology Yokosuka, Japan.

ABSTRACT
Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments. The isolates were phylogenetically assigned to Alphaproteobacteria, Gammmaproteobacteria, and Bacilli. Some of the isolates exhibited high enantioselective degradation activities to various DAAs. In particular, the Alphaproteobacteria Nautella sp. strain A04V exhibited robust growth in minimal medium supplemented with D-Val as a sole carbon and nitrogen source, whereas its growth was poor on minimal medium supplemented with L-Val instead of D-Val. Its growth was facilitated most when racemic mixtures of valine were used. In contrast, the Nautella strains isolated from shallow-sea grew only with L-Val. No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.

No MeSH data available.


Related in: MedlinePlus