Limits...
Enantioselective Utilization of D-Amino Acids by Deep-Sea Microorganisms.

Kubota T, Kobayashi T, Nunoura T, Maruyama F, Deguchi S - Front Microbiol (2016)

Bottom Line: Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments.In contrast, the Nautella strains isolated from shallow-sea grew only with L-Val.No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology Yokosuka, Japan.

ABSTRACT
Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments. The isolates were phylogenetically assigned to Alphaproteobacteria, Gammmaproteobacteria, and Bacilli. Some of the isolates exhibited high enantioselective degradation activities to various DAAs. In particular, the Alphaproteobacteria Nautella sp. strain A04V exhibited robust growth in minimal medium supplemented with D-Val as a sole carbon and nitrogen source, whereas its growth was poor on minimal medium supplemented with L-Val instead of D-Val. Its growth was facilitated most when racemic mixtures of valine were used. In contrast, the Nautella strains isolated from shallow-sea grew only with L-Val. No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.

No MeSH data available.


Related in: MedlinePlus

Growth curves of Nautella strains in the presence of D, L-valine. Strains A04V (A) and LMG 24365 (B) in the presence of various concentrations of D-Val and L-Val: D:L = 0:6 mM (open circles), D:L = 1:5 mM (open triangles), D:L = 2:4 mM (open squares), D:L = 3:3 mM (light-gray diamonds), D:L = 4:2 mM (filled squares), D:L = 5:1 mM (filled triangles), and D:L = 6:0 mM (filled circles).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4836201&req=5

Figure 5: Growth curves of Nautella strains in the presence of D, L-valine. Strains A04V (A) and LMG 24365 (B) in the presence of various concentrations of D-Val and L-Val: D:L = 0:6 mM (open circles), D:L = 1:5 mM (open triangles), D:L = 2:4 mM (open squares), D:L = 3:3 mM (light-gray diamonds), D:L = 4:2 mM (filled squares), D:L = 5:1 mM (filled triangles), and D:L = 6:0 mM (filled circles).

Mentions: Moreover, strain A04V exhibited unique growth behavior in the presence of racemic Val, i.e., a mixture of L- and D-Val (the final total Val concentration was 6 mM), as the sole carbon and nitrogen source (Figure 5A). Growth was robust in the presence of both Val enantiomers, whereas growth was weak with each separate enantiomer of Val. In contrast, the type strain of N. italica (strain LMG24365), which was isolated from a shallow-sea environment (Vandecandelaere et al., 2009), grew only with L-Val, and not with D-Val or racemic mixtures (Figure 5B). As strain A04V and N. italica LMG24365 share 100% similarity in terms of its 16S rRNA genes, the capacity for utilizing D-Val as the sole carbon and nitrogen source for growth is not a common feature of this genus.


Enantioselective Utilization of D-Amino Acids by Deep-Sea Microorganisms.

Kubota T, Kobayashi T, Nunoura T, Maruyama F, Deguchi S - Front Microbiol (2016)

Growth curves of Nautella strains in the presence of D, L-valine. Strains A04V (A) and LMG 24365 (B) in the presence of various concentrations of D-Val and L-Val: D:L = 0:6 mM (open circles), D:L = 1:5 mM (open triangles), D:L = 2:4 mM (open squares), D:L = 3:3 mM (light-gray diamonds), D:L = 4:2 mM (filled squares), D:L = 5:1 mM (filled triangles), and D:L = 6:0 mM (filled circles).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4836201&req=5

Figure 5: Growth curves of Nautella strains in the presence of D, L-valine. Strains A04V (A) and LMG 24365 (B) in the presence of various concentrations of D-Val and L-Val: D:L = 0:6 mM (open circles), D:L = 1:5 mM (open triangles), D:L = 2:4 mM (open squares), D:L = 3:3 mM (light-gray diamonds), D:L = 4:2 mM (filled squares), D:L = 5:1 mM (filled triangles), and D:L = 6:0 mM (filled circles).
Mentions: Moreover, strain A04V exhibited unique growth behavior in the presence of racemic Val, i.e., a mixture of L- and D-Val (the final total Val concentration was 6 mM), as the sole carbon and nitrogen source (Figure 5A). Growth was robust in the presence of both Val enantiomers, whereas growth was weak with each separate enantiomer of Val. In contrast, the type strain of N. italica (strain LMG24365), which was isolated from a shallow-sea environment (Vandecandelaere et al., 2009), grew only with L-Val, and not with D-Val or racemic mixtures (Figure 5B). As strain A04V and N. italica LMG24365 share 100% similarity in terms of its 16S rRNA genes, the capacity for utilizing D-Val as the sole carbon and nitrogen source for growth is not a common feature of this genus.

Bottom Line: Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments.In contrast, the Nautella strains isolated from shallow-sea grew only with L-Val.No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology Yokosuka, Japan.

ABSTRACT
Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments. The isolates were phylogenetically assigned to Alphaproteobacteria, Gammmaproteobacteria, and Bacilli. Some of the isolates exhibited high enantioselective degradation activities to various DAAs. In particular, the Alphaproteobacteria Nautella sp. strain A04V exhibited robust growth in minimal medium supplemented with D-Val as a sole carbon and nitrogen source, whereas its growth was poor on minimal medium supplemented with L-Val instead of D-Val. Its growth was facilitated most when racemic mixtures of valine were used. In contrast, the Nautella strains isolated from shallow-sea grew only with L-Val. No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.

No MeSH data available.


Related in: MedlinePlus