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Enantioselective Utilization of D-Amino Acids by Deep-Sea Microorganisms.

Kubota T, Kobayashi T, Nunoura T, Maruyama F, Deguchi S - Front Microbiol (2016)

Bottom Line: Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments.In contrast, the Nautella strains isolated from shallow-sea grew only with L-Val.No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology Yokosuka, Japan.

ABSTRACT
Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments. The isolates were phylogenetically assigned to Alphaproteobacteria, Gammmaproteobacteria, and Bacilli. Some of the isolates exhibited high enantioselective degradation activities to various DAAs. In particular, the Alphaproteobacteria Nautella sp. strain A04V exhibited robust growth in minimal medium supplemented with D-Val as a sole carbon and nitrogen source, whereas its growth was poor on minimal medium supplemented with L-Val instead of D-Val. Its growth was facilitated most when racemic mixtures of valine were used. In contrast, the Nautella strains isolated from shallow-sea grew only with L-Val. No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.

No MeSH data available.


Related in: MedlinePlus

Growth curves of Nautella sp. strain A04V. (A) In the presence of 4 mM amino acids; L-Val (open circle), D-Val (closed circle), (B) L-Leu (open square) and D-Leu (closed square).
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Figure 4: Growth curves of Nautella sp. strain A04V. (A) In the presence of 4 mM amino acids; L-Val (open circle), D-Val (closed circle), (B) L-Leu (open square) and D-Leu (closed square).

Mentions: During its growth with branched-chain AAs such as Val and Leu, strain A04V preferred the D-forms for growth rather than the L-forms (Figure 4). The preference for the D-forms of AAs was not observed when we tested other AAs such as Ala, Glu, and Phe as sole carbon and nitrogen sources (Figure S1). The optimum D-Val concentration for growth was 5–6 mM. Adverse effects of high concentrations of DAAs have been reported previously in a variety of bacteria (Teeri and Josselyn, 1953; Bopp, 1965; Champney and Jensen, 1970; Cosloy and McFall, 1973; Yabu and Huempfner, 1974; White, 1979). The growth of strain A04V was also completely inhibited in the presence of >10 mM D-Val. In contrast, high concentrations (< 100 mM) of L-Val did not inhibit the growth of this strain at all. High concentrations of DAAs induce significant morphological changes for Vibrio cholerae cells (Lam et al., 2009), but such effect was not observed for strain A04V even when it was incubated with a lethal level of D-Val (10 mM).


Enantioselective Utilization of D-Amino Acids by Deep-Sea Microorganisms.

Kubota T, Kobayashi T, Nunoura T, Maruyama F, Deguchi S - Front Microbiol (2016)

Growth curves of Nautella sp. strain A04V. (A) In the presence of 4 mM amino acids; L-Val (open circle), D-Val (closed circle), (B) L-Leu (open square) and D-Leu (closed square).
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Related In: Results  -  Collection

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Figure 4: Growth curves of Nautella sp. strain A04V. (A) In the presence of 4 mM amino acids; L-Val (open circle), D-Val (closed circle), (B) L-Leu (open square) and D-Leu (closed square).
Mentions: During its growth with branched-chain AAs such as Val and Leu, strain A04V preferred the D-forms for growth rather than the L-forms (Figure 4). The preference for the D-forms of AAs was not observed when we tested other AAs such as Ala, Glu, and Phe as sole carbon and nitrogen sources (Figure S1). The optimum D-Val concentration for growth was 5–6 mM. Adverse effects of high concentrations of DAAs have been reported previously in a variety of bacteria (Teeri and Josselyn, 1953; Bopp, 1965; Champney and Jensen, 1970; Cosloy and McFall, 1973; Yabu and Huempfner, 1974; White, 1979). The growth of strain A04V was also completely inhibited in the presence of >10 mM D-Val. In contrast, high concentrations (< 100 mM) of L-Val did not inhibit the growth of this strain at all. High concentrations of DAAs induce significant morphological changes for Vibrio cholerae cells (Lam et al., 2009), but such effect was not observed for strain A04V even when it was incubated with a lethal level of D-Val (10 mM).

Bottom Line: Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments.In contrast, the Nautella strains isolated from shallow-sea grew only with L-Val.No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.

View Article: PubMed Central - PubMed

Affiliation: Research and Development Center for Marine Biosciences, Japan Agency for Marine-Earth Science and Technology Yokosuka, Japan.

ABSTRACT
Microorganisms that utilize various D-amino acids (DAAs) were successfully isolated from deep-sea sediments. The isolates were phylogenetically assigned to Alphaproteobacteria, Gammmaproteobacteria, and Bacilli. Some of the isolates exhibited high enantioselective degradation activities to various DAAs. In particular, the Alphaproteobacteria Nautella sp. strain A04V exhibited robust growth in minimal medium supplemented with D-Val as a sole carbon and nitrogen source, whereas its growth was poor on minimal medium supplemented with L-Val instead of D-Val. Its growth was facilitated most when racemic mixtures of valine were used. In contrast, the Nautella strains isolated from shallow-sea grew only with L-Val. No significant differences were found among the strains in the genome sequences including genes possibly related to DAA metabolisms.

No MeSH data available.


Related in: MedlinePlus