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Tick-borne pathogens of zoonotic and veterinary importance in Nigerian cattle.

Lorusso V, Wijnveld M, Majekodunmi AO, Dongkum C, Fajinmi A, Dogo AG, Thrusfield M, Mugenyi A, Vaumourin E, Igweh AC, Jongejan F, Welburn SC, Picozzi K - Parasit Vectors (2016)

Bottom Line: In total, 561/704 (82.6%) animals were found infected, with 465 (69.6%) of them being infected by two or more microorganisms, with up to 77 possible combinations of pathogens detected.Calves were found significantly less infected than juvenile and adult cattle.The high prevalence recorded for T. mutans, T. velifera, A. marginale, T. taurotragi and Anaplasma sp. (Omatjenne), suggests they may be endemically established in Nigeria, whereas the lower prevalence recorded for other microorganisms (i.e. A. centrale and B. bovis) highlights a less stable epidemiological scenario, requiring further investigations.

View Article: PubMed Central - PubMed

Affiliation: Division of Infection and Pathway Medicine, School of Biomedical Sciences, The University of Edinburgh, Edinburgh, UK.

ABSTRACT

Background: Ticks and tick-borne diseases undermine cattle fitness and productivity in the whole of sub-Saharan Africa, including Nigeria. In this West African country, cattle are challenged by numerous tick species, especially during the wet season. Consequently, several TBDs are known to be endemic in Nigerian cattle, including anaplasmosis, babesiosis, cowdriosis and theilerioris (by Theileria mutans and Theileria velifera). To date, all investigations on cattle TBDs in Nigeria have been based on cytological examinations and/or on serological methods. This study aimed to ascertain the occurrence of tick-borne pathogens of veterinary and zoonotic importance in cattle in Nigeria using molecular approaches.

Methods: In October 2008, 704 whole blood samples were collected from indigenous cattle in the Plateau State, Nigeria. Analysis for tick-borne pathogens was conducted by means of PCR-based reverse line blotting (RLB) and sequencing targeting a panel of five genera of microorganisms (i.e. Babesia, Theileria, Anaplasma, Ehrlichia and Rickettsia spp.).

Results: In total, 561/704 (82.6%) animals were found infected, with 465 (69.6%) of them being infected by two or more microorganisms, with up to 77 possible combinations of pathogens detected. Theileria mutans was the most prevalent microorganism (66.3%), followed by Theileria velifera (52.4%), Theileria taurotragi (39.5%), Anaplasma marginale (39.1%), Anaplasma sp. (Omatjenne) (34.7%), Babesia bigemina (7.9%), Anaplasma centrale (6.3%), Anaplasma platys (3.9%), Rickettsia massiliae (3.5%), Babesia bovis (2.0%) and Ehrlichia ruminantium (1.1%). Calves were found significantly less infected than juvenile and adult cattle.

Conclusions: This study provides updated, molecular-based information on cattle TBDs in Nigeria. The molecular approach employed allowed the diagnosis of numerous positive cases including carrier statuses, multiple infections and novel pathogen detections within the indigenous cattle population. Moreover, the RLB method here described enabled the detection of veterinary agents not only pertaining to bovine health, including also those of zoonotic importance. The high prevalence recorded for T. mutans, T. velifera, A. marginale, T. taurotragi and Anaplasma sp. (Omatjenne), suggests they may be endemically established in Nigeria, whereas the lower prevalence recorded for other microorganisms (i.e. A. centrale and B. bovis) highlights a less stable epidemiological scenario, requiring further investigations.

No MeSH data available.


Related in: MedlinePlus

Prevalence (%) of each tick-borne pathogen in relation to its primary competent vector ticks. (1) = A. variegatum for T. mutans, T. velifera and E. ruminantium; (2) = Rhipicephalus spp. for T. taurotragi; (3) = Rhipicephalus (Boophilus) spp. for A. marginale, B. bigemina, A. centrale and B. bovis; (4) = H. truncatum, presumably, for Anaplasma sp. (Omatjenne); (5) = Rh. simus for A. marginale and A. centrale; (6) = Rh. sanguineus (sensu lato) for A. platys and Rickettsia spp. (R. massiliae according to 16S rDNA sequencing). Vector competence for the transmission of T. taurotragi, Anaplasma sp. (Omatjenne), A. platys, R. massiliae; Anaplasma spp. in Rh. simus Group and B. bovis in Rh. (Bo.) spp. in Nigeria needs to be further confirmed
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Fig3: Prevalence (%) of each tick-borne pathogen in relation to its primary competent vector ticks. (1) = A. variegatum for T. mutans, T. velifera and E. ruminantium; (2) = Rhipicephalus spp. for T. taurotragi; (3) = Rhipicephalus (Boophilus) spp. for A. marginale, B. bigemina, A. centrale and B. bovis; (4) = H. truncatum, presumably, for Anaplasma sp. (Omatjenne); (5) = Rh. simus for A. marginale and A. centrale; (6) = Rh. sanguineus (sensu lato) for A. platys and Rickettsia spp. (R. massiliae according to 16S rDNA sequencing). Vector competence for the transmission of T. taurotragi, Anaplasma sp. (Omatjenne), A. platys, R. massiliae; Anaplasma spp. in Rh. simus Group and B. bovis in Rh. (Bo.) spp. in Nigeria needs to be further confirmed

Mentions: Theileria mutans was the most prevalent microorganism (n = 435/704, 95 % CI: 62.1–70.4 %), followed by Theileria velifera (n = 348/704, 95 % CI: 47.9–56.9 %), Theileria taurotragi (n = 260/704, 95 % CI: 35.1–43.9 %), Anaplasma marginale (n = 268/704, 95 % CI: 34.8–43.5 %), Anaplasma sp. (Omatjenne) (n = 239/704, 95 % CI: 30.5–38.9 %), Babesia bigemina (n = 57/704, 95 % CI: 5.6–10.2 %), Anaplasma centrale (n = 57/704, 95 % CI: 4.2–8.3 %), Ehrlichia/Anaplasma spp. (n = 27/704, 95 % CI: 2.1–5.7 %), Rickettsia spp. (n = 19/704, 95 % CI: 1.7–5.2 %), Babesia bovis (n = 16/704, 95 % CI: 1.0–2.9 %), Ehrlichia ruminantium (n = 8/704, 95 % CI: 0.2–1.9 %) (see also Fig. 3).Fig. 3


Tick-borne pathogens of zoonotic and veterinary importance in Nigerian cattle.

Lorusso V, Wijnveld M, Majekodunmi AO, Dongkum C, Fajinmi A, Dogo AG, Thrusfield M, Mugenyi A, Vaumourin E, Igweh AC, Jongejan F, Welburn SC, Picozzi K - Parasit Vectors (2016)

Prevalence (%) of each tick-borne pathogen in relation to its primary competent vector ticks. (1) = A. variegatum for T. mutans, T. velifera and E. ruminantium; (2) = Rhipicephalus spp. for T. taurotragi; (3) = Rhipicephalus (Boophilus) spp. for A. marginale, B. bigemina, A. centrale and B. bovis; (4) = H. truncatum, presumably, for Anaplasma sp. (Omatjenne); (5) = Rh. simus for A. marginale and A. centrale; (6) = Rh. sanguineus (sensu lato) for A. platys and Rickettsia spp. (R. massiliae according to 16S rDNA sequencing). Vector competence for the transmission of T. taurotragi, Anaplasma sp. (Omatjenne), A. platys, R. massiliae; Anaplasma spp. in Rh. simus Group and B. bovis in Rh. (Bo.) spp. in Nigeria needs to be further confirmed
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4836144&req=5

Fig3: Prevalence (%) of each tick-borne pathogen in relation to its primary competent vector ticks. (1) = A. variegatum for T. mutans, T. velifera and E. ruminantium; (2) = Rhipicephalus spp. for T. taurotragi; (3) = Rhipicephalus (Boophilus) spp. for A. marginale, B. bigemina, A. centrale and B. bovis; (4) = H. truncatum, presumably, for Anaplasma sp. (Omatjenne); (5) = Rh. simus for A. marginale and A. centrale; (6) = Rh. sanguineus (sensu lato) for A. platys and Rickettsia spp. (R. massiliae according to 16S rDNA sequencing). Vector competence for the transmission of T. taurotragi, Anaplasma sp. (Omatjenne), A. platys, R. massiliae; Anaplasma spp. in Rh. simus Group and B. bovis in Rh. (Bo.) spp. in Nigeria needs to be further confirmed
Mentions: Theileria mutans was the most prevalent microorganism (n = 435/704, 95 % CI: 62.1–70.4 %), followed by Theileria velifera (n = 348/704, 95 % CI: 47.9–56.9 %), Theileria taurotragi (n = 260/704, 95 % CI: 35.1–43.9 %), Anaplasma marginale (n = 268/704, 95 % CI: 34.8–43.5 %), Anaplasma sp. (Omatjenne) (n = 239/704, 95 % CI: 30.5–38.9 %), Babesia bigemina (n = 57/704, 95 % CI: 5.6–10.2 %), Anaplasma centrale (n = 57/704, 95 % CI: 4.2–8.3 %), Ehrlichia/Anaplasma spp. (n = 27/704, 95 % CI: 2.1–5.7 %), Rickettsia spp. (n = 19/704, 95 % CI: 1.7–5.2 %), Babesia bovis (n = 16/704, 95 % CI: 1.0–2.9 %), Ehrlichia ruminantium (n = 8/704, 95 % CI: 0.2–1.9 %) (see also Fig. 3).Fig. 3

Bottom Line: In total, 561/704 (82.6%) animals were found infected, with 465 (69.6%) of them being infected by two or more microorganisms, with up to 77 possible combinations of pathogens detected.Calves were found significantly less infected than juvenile and adult cattle.The high prevalence recorded for T. mutans, T. velifera, A. marginale, T. taurotragi and Anaplasma sp. (Omatjenne), suggests they may be endemically established in Nigeria, whereas the lower prevalence recorded for other microorganisms (i.e. A. centrale and B. bovis) highlights a less stable epidemiological scenario, requiring further investigations.

View Article: PubMed Central - PubMed

Affiliation: Division of Infection and Pathway Medicine, School of Biomedical Sciences, The University of Edinburgh, Edinburgh, UK.

ABSTRACT

Background: Ticks and tick-borne diseases undermine cattle fitness and productivity in the whole of sub-Saharan Africa, including Nigeria. In this West African country, cattle are challenged by numerous tick species, especially during the wet season. Consequently, several TBDs are known to be endemic in Nigerian cattle, including anaplasmosis, babesiosis, cowdriosis and theilerioris (by Theileria mutans and Theileria velifera). To date, all investigations on cattle TBDs in Nigeria have been based on cytological examinations and/or on serological methods. This study aimed to ascertain the occurrence of tick-borne pathogens of veterinary and zoonotic importance in cattle in Nigeria using molecular approaches.

Methods: In October 2008, 704 whole blood samples were collected from indigenous cattle in the Plateau State, Nigeria. Analysis for tick-borne pathogens was conducted by means of PCR-based reverse line blotting (RLB) and sequencing targeting a panel of five genera of microorganisms (i.e. Babesia, Theileria, Anaplasma, Ehrlichia and Rickettsia spp.).

Results: In total, 561/704 (82.6%) animals were found infected, with 465 (69.6%) of them being infected by two or more microorganisms, with up to 77 possible combinations of pathogens detected. Theileria mutans was the most prevalent microorganism (66.3%), followed by Theileria velifera (52.4%), Theileria taurotragi (39.5%), Anaplasma marginale (39.1%), Anaplasma sp. (Omatjenne) (34.7%), Babesia bigemina (7.9%), Anaplasma centrale (6.3%), Anaplasma platys (3.9%), Rickettsia massiliae (3.5%), Babesia bovis (2.0%) and Ehrlichia ruminantium (1.1%). Calves were found significantly less infected than juvenile and adult cattle.

Conclusions: This study provides updated, molecular-based information on cattle TBDs in Nigeria. The molecular approach employed allowed the diagnosis of numerous positive cases including carrier statuses, multiple infections and novel pathogen detections within the indigenous cattle population. Moreover, the RLB method here described enabled the detection of veterinary agents not only pertaining to bovine health, including also those of zoonotic importance. The high prevalence recorded for T. mutans, T. velifera, A. marginale, T. taurotragi and Anaplasma sp. (Omatjenne), suggests they may be endemically established in Nigeria, whereas the lower prevalence recorded for other microorganisms (i.e. A. centrale and B. bovis) highlights a less stable epidemiological scenario, requiring further investigations.

No MeSH data available.


Related in: MedlinePlus