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Challenges and opportunities of bovine milk analysis by mass spectrometry.

Verma A, Ambatipudi K - Clin Proteomics (2016)

Bottom Line: Although analyses of different milk components (e.g. proteins, lipids) pose huge challenges, the use of mass spectrometric (MS)-based techniques is steadily improving our understanding of the complexity of the biological traits that effect milk yield and its components to meet the global demand arising from population growth.In addition, different milk constituents have various applications in veterinary research and medicine, including early disease diagnosis.The aim of the review is to present an overview of the progress made in MS-based analysis of milk, and suggest a multi-pronged MS strategy to better explore different milk components for translational and clinical utilities.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667 India.

ABSTRACT
Bovine milk and its products (e.g. cheese, yoghurt) are an important part of human diet with beneficial effects for all ages. Although analyses of different milk components (e.g. proteins, lipids) pose huge challenges, the use of mass spectrometric (MS)-based techniques is steadily improving our understanding of the complexity of the biological traits that effect milk yield and its components to meet the global demand arising from population growth. In addition, different milk constituents have various applications in veterinary research and medicine, including early disease diagnosis. The aim of the review is to present an overview of the progress made in MS-based analysis of milk, and suggest a multi-pronged MS strategy to better explore different milk components for translational and clinical utilities.

No MeSH data available.


Possible pathways for protein identification. A combination of strategies for characterization of bovine milk whey and casein for maximizing successful protein identification
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Fig2: Possible pathways for protein identification. A combination of strategies for characterization of bovine milk whey and casein for maximizing successful protein identification

Mentions: If the above techniques fail to provide any positive protein identifications, de novo sequencing followed by BLAST searching provides an alternative identification strategy [177, 178]. By this analysis the amino acid sequence is obtained by evaluating the mass difference between two adjacent y- and b-ion series in the fragmentation spectra of the precursor ion [173]. Alternatively, specialized software can be used to create amino acid sequence to interpret tandem mass spectra of peptides [178]. Figure 2 shows possible ways of sample analysis to maximize confident protein identification.Fig. 2


Challenges and opportunities of bovine milk analysis by mass spectrometry.

Verma A, Ambatipudi K - Clin Proteomics (2016)

Possible pathways for protein identification. A combination of strategies for characterization of bovine milk whey and casein for maximizing successful protein identification
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4836106&req=5

Fig2: Possible pathways for protein identification. A combination of strategies for characterization of bovine milk whey and casein for maximizing successful protein identification
Mentions: If the above techniques fail to provide any positive protein identifications, de novo sequencing followed by BLAST searching provides an alternative identification strategy [177, 178]. By this analysis the amino acid sequence is obtained by evaluating the mass difference between two adjacent y- and b-ion series in the fragmentation spectra of the precursor ion [173]. Alternatively, specialized software can be used to create amino acid sequence to interpret tandem mass spectra of peptides [178]. Figure 2 shows possible ways of sample analysis to maximize confident protein identification.Fig. 2

Bottom Line: Although analyses of different milk components (e.g. proteins, lipids) pose huge challenges, the use of mass spectrometric (MS)-based techniques is steadily improving our understanding of the complexity of the biological traits that effect milk yield and its components to meet the global demand arising from population growth.In addition, different milk constituents have various applications in veterinary research and medicine, including early disease diagnosis.The aim of the review is to present an overview of the progress made in MS-based analysis of milk, and suggest a multi-pronged MS strategy to better explore different milk components for translational and clinical utilities.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667 India.

ABSTRACT
Bovine milk and its products (e.g. cheese, yoghurt) are an important part of human diet with beneficial effects for all ages. Although analyses of different milk components (e.g. proteins, lipids) pose huge challenges, the use of mass spectrometric (MS)-based techniques is steadily improving our understanding of the complexity of the biological traits that effect milk yield and its components to meet the global demand arising from population growth. In addition, different milk constituents have various applications in veterinary research and medicine, including early disease diagnosis. The aim of the review is to present an overview of the progress made in MS-based analysis of milk, and suggest a multi-pronged MS strategy to better explore different milk components for translational and clinical utilities.

No MeSH data available.