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ABA-mediated responses to water deficit separate grapevine genotypes by their genetic background.

Rossdeutsch L, Edwards E, Cookson SJ, Barrieu F, Gambetta GA, Delrot S, Ollat N - BMC Plant Biol. (2016)

Bottom Line: The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses.In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

View Article: PubMed Central - PubMed

Affiliation: UMR EGFV, ISVV-INRA, 210 chemin de Leysotte, 33882, Villenave d'Ornon, France.

ABSTRACT

Background: ABA-mediated processes are involved in plant responses to water deficit, especially the control of stomatal opening. However in grapevine it is not known if these processes participate in the phenotypic variation in drought adaptation existing between genotypes. To elucidate this question, the response to short-term water-deficit was analysed in roots and shoots of nine Vitis genotypes differing in their drought adaptation in the field. The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.

Results: Although transpiration and ABA responses were well-conserved among the genotypes, multifactorial analyses separated Vitis vinifera varieties and V. berlandieri x V. rupestris hybrids (all considered drought tolerant) from the other genotypes studied. Generally, V. vinifera varieties, followed by V. berlandieri x V. rupestris hybrids, displayed more pronounced responses to water-deficit in comparison to the other genotypes. However, changes in transcript abundance in roots were more pronounced for Vitis hybrids than V. vinifera genotypes. Changes in the expression of the cornerstone ABA biosynthetic gene VviNCED1, and the ABA transcriptional regulator VviABF1, were associated with the response of V. vinifera genotypes, while changes in VviNCED2 abundance were associated with the response of other Vitis genotypes. In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses. Interestingly, the expression of VviSnRK2.6 (an AtOST1 ortholog) was constitutively lower in roots and leaves of V. vinifera genotypes and higher in roots of V. berlandieri x V. rupestris hybrids.

Conclusions: This study highlights that Vitis genotypes exhibiting different levels of drought adaptation differ in key steps involved in ABA metabolism and signalling; both under well-watered conditions and in response to water-deficit. In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

No MeSH data available.


Related in: MedlinePlus

Summarized view of the responses recorded in the experiment for the literature-based tolerant Vitis genotypes. ABA-related gene expression, metabolite concentration and transpiration sensitivity to ABA after 4 days of withholding irrigation are illustrated for the genotypes defined in literature as drought tolerant i.e. V. berlandieri x V. rupestris hybrids (left) and V. vinifera (right). Colours indicate genes expression values and ABA-related metabolite concentration for 140Ru and Grenache scaled between the lowest and the highest values, 4 days after withholding irrigation for all genotypes and tissues. Warning symbols indicate the intra-group variability when it is significant between 140Ru and 110R on one side and between Grenache and Syrah on the other side, according to Fig. 2 & 3a and Additional files 5 & 6
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Fig8: Summarized view of the responses recorded in the experiment for the literature-based tolerant Vitis genotypes. ABA-related gene expression, metabolite concentration and transpiration sensitivity to ABA after 4 days of withholding irrigation are illustrated for the genotypes defined in literature as drought tolerant i.e. V. berlandieri x V. rupestris hybrids (left) and V. vinifera (right). Colours indicate genes expression values and ABA-related metabolite concentration for 140Ru and Grenache scaled between the lowest and the highest values, 4 days after withholding irrigation for all genotypes and tissues. Warning symbols indicate the intra-group variability when it is significant between 140Ru and 110R on one side and between Grenache and Syrah on the other side, according to Fig. 2 & 3a and Additional files 5 & 6

Mentions: The V. vinifera genotypes displayed more pronounced transcriptional responses to the water-deficit treatment than the other genotypes, followed by the V. berlandieri x V. rupestris hybrids and 41B (a V. berlandieri x V. vinifera hybrid). These changes are summarized in Fig. 8. The response of V. vinifera genotypes to water-deficit was mainly associated with changes in abundance of VviNCED1 in leaves and roots, and VviHyds and VviABFs in leaves. For V. berlandieri x V. rupestris hybrids and 41B, the intermediate response was associated with the abundance of VviNCED2 and VviSnRK2.6 in leaves and, VviNCED2, VviHyd2, VviPP2C9, and VviABF1 in roots. Own rooted V. vinifera are considered to better tolerate drought than when grafted on American hybrids [59]. This high drought tolerance could be associated with the ability to regulate the expression of genes that control ABA responses in leaves observed in the present study. In V. berlandieri x V rupestris hybrids and 41B, which are characterized as drought tolerant rootstocks [13, 56], the response appears to have a relatively stronger root component. The ABA receptors, VviRCAR5 and VviRCAR6, were not identified as key component of the variability of water-deficit responses between the genotypes. The responses of ABA concentration and transpiration to plant water potential were also more pronounced for some of these tolerant genotypes such as Grenache, 140Ru and 41B.Fig. 8


ABA-mediated responses to water deficit separate grapevine genotypes by their genetic background.

Rossdeutsch L, Edwards E, Cookson SJ, Barrieu F, Gambetta GA, Delrot S, Ollat N - BMC Plant Biol. (2016)

Summarized view of the responses recorded in the experiment for the literature-based tolerant Vitis genotypes. ABA-related gene expression, metabolite concentration and transpiration sensitivity to ABA after 4 days of withholding irrigation are illustrated for the genotypes defined in literature as drought tolerant i.e. V. berlandieri x V. rupestris hybrids (left) and V. vinifera (right). Colours indicate genes expression values and ABA-related metabolite concentration for 140Ru and Grenache scaled between the lowest and the highest values, 4 days after withholding irrigation for all genotypes and tissues. Warning symbols indicate the intra-group variability when it is significant between 140Ru and 110R on one side and between Grenache and Syrah on the other side, according to Fig. 2 & 3a and Additional files 5 & 6
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4836075&req=5

Fig8: Summarized view of the responses recorded in the experiment for the literature-based tolerant Vitis genotypes. ABA-related gene expression, metabolite concentration and transpiration sensitivity to ABA after 4 days of withholding irrigation are illustrated for the genotypes defined in literature as drought tolerant i.e. V. berlandieri x V. rupestris hybrids (left) and V. vinifera (right). Colours indicate genes expression values and ABA-related metabolite concentration for 140Ru and Grenache scaled between the lowest and the highest values, 4 days after withholding irrigation for all genotypes and tissues. Warning symbols indicate the intra-group variability when it is significant between 140Ru and 110R on one side and between Grenache and Syrah on the other side, according to Fig. 2 & 3a and Additional files 5 & 6
Mentions: The V. vinifera genotypes displayed more pronounced transcriptional responses to the water-deficit treatment than the other genotypes, followed by the V. berlandieri x V. rupestris hybrids and 41B (a V. berlandieri x V. vinifera hybrid). These changes are summarized in Fig. 8. The response of V. vinifera genotypes to water-deficit was mainly associated with changes in abundance of VviNCED1 in leaves and roots, and VviHyds and VviABFs in leaves. For V. berlandieri x V. rupestris hybrids and 41B, the intermediate response was associated with the abundance of VviNCED2 and VviSnRK2.6 in leaves and, VviNCED2, VviHyd2, VviPP2C9, and VviABF1 in roots. Own rooted V. vinifera are considered to better tolerate drought than when grafted on American hybrids [59]. This high drought tolerance could be associated with the ability to regulate the expression of genes that control ABA responses in leaves observed in the present study. In V. berlandieri x V rupestris hybrids and 41B, which are characterized as drought tolerant rootstocks [13, 56], the response appears to have a relatively stronger root component. The ABA receptors, VviRCAR5 and VviRCAR6, were not identified as key component of the variability of water-deficit responses between the genotypes. The responses of ABA concentration and transpiration to plant water potential were also more pronounced for some of these tolerant genotypes such as Grenache, 140Ru and 41B.Fig. 8

Bottom Line: The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses.In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

View Article: PubMed Central - PubMed

Affiliation: UMR EGFV, ISVV-INRA, 210 chemin de Leysotte, 33882, Villenave d'Ornon, France.

ABSTRACT

Background: ABA-mediated processes are involved in plant responses to water deficit, especially the control of stomatal opening. However in grapevine it is not known if these processes participate in the phenotypic variation in drought adaptation existing between genotypes. To elucidate this question, the response to short-term water-deficit was analysed in roots and shoots of nine Vitis genotypes differing in their drought adaptation in the field. The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.

Results: Although transpiration and ABA responses were well-conserved among the genotypes, multifactorial analyses separated Vitis vinifera varieties and V. berlandieri x V. rupestris hybrids (all considered drought tolerant) from the other genotypes studied. Generally, V. vinifera varieties, followed by V. berlandieri x V. rupestris hybrids, displayed more pronounced responses to water-deficit in comparison to the other genotypes. However, changes in transcript abundance in roots were more pronounced for Vitis hybrids than V. vinifera genotypes. Changes in the expression of the cornerstone ABA biosynthetic gene VviNCED1, and the ABA transcriptional regulator VviABF1, were associated with the response of V. vinifera genotypes, while changes in VviNCED2 abundance were associated with the response of other Vitis genotypes. In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses. Interestingly, the expression of VviSnRK2.6 (an AtOST1 ortholog) was constitutively lower in roots and leaves of V. vinifera genotypes and higher in roots of V. berlandieri x V. rupestris hybrids.

Conclusions: This study highlights that Vitis genotypes exhibiting different levels of drought adaptation differ in key steps involved in ABA metabolism and signalling; both under well-watered conditions and in response to water-deficit. In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

No MeSH data available.


Related in: MedlinePlus