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ABA-mediated responses to water deficit separate grapevine genotypes by their genetic background.

Rossdeutsch L, Edwards E, Cookson SJ, Barrieu F, Gambetta GA, Delrot S, Ollat N - BMC Plant Biol. (2016)

Bottom Line: The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses.In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

View Article: PubMed Central - PubMed

Affiliation: UMR EGFV, ISVV-INRA, 210 chemin de Leysotte, 33882, Villenave d'Ornon, France.

ABSTRACT

Background: ABA-mediated processes are involved in plant responses to water deficit, especially the control of stomatal opening. However in grapevine it is not known if these processes participate in the phenotypic variation in drought adaptation existing between genotypes. To elucidate this question, the response to short-term water-deficit was analysed in roots and shoots of nine Vitis genotypes differing in their drought adaptation in the field. The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.

Results: Although transpiration and ABA responses were well-conserved among the genotypes, multifactorial analyses separated Vitis vinifera varieties and V. berlandieri x V. rupestris hybrids (all considered drought tolerant) from the other genotypes studied. Generally, V. vinifera varieties, followed by V. berlandieri x V. rupestris hybrids, displayed more pronounced responses to water-deficit in comparison to the other genotypes. However, changes in transcript abundance in roots were more pronounced for Vitis hybrids than V. vinifera genotypes. Changes in the expression of the cornerstone ABA biosynthetic gene VviNCED1, and the ABA transcriptional regulator VviABF1, were associated with the response of V. vinifera genotypes, while changes in VviNCED2 abundance were associated with the response of other Vitis genotypes. In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses. Interestingly, the expression of VviSnRK2.6 (an AtOST1 ortholog) was constitutively lower in roots and leaves of V. vinifera genotypes and higher in roots of V. berlandieri x V. rupestris hybrids.

Conclusions: This study highlights that Vitis genotypes exhibiting different levels of drought adaptation differ in key steps involved in ABA metabolism and signalling; both under well-watered conditions and in response to water-deficit. In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

No MeSH data available.


Related in: MedlinePlus

Heatmaps of the abundance of transcripts for studied genes and their variations with water deficit. The abundance of transcripts for the genes associated with abscisic acid was recorded in the leaves and roots of nine grapevine genotypes during a water-deficit treatment. Transcript abundance at day 1 after withholding irrigation (non-stressed plants) (a), green shade indicates the level of expression relative to the lowest value (n = 3). Transcript abundance changes from day 1 to day 4 after withholding irrigation (water-stressed plants) (b), the blue and red shades indicate the extent of gene repression and induction respectively (n = 3). Blocks of squares show the level of gene expression in the leaves and roots of nine different grapevine genotypes (c) for each gene studied
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Fig5: Heatmaps of the abundance of transcripts for studied genes and their variations with water deficit. The abundance of transcripts for the genes associated with abscisic acid was recorded in the leaves and roots of nine grapevine genotypes during a water-deficit treatment. Transcript abundance at day 1 after withholding irrigation (non-stressed plants) (a), green shade indicates the level of expression relative to the lowest value (n = 3). Transcript abundance changes from day 1 to day 4 after withholding irrigation (water-stressed plants) (b), the blue and red shades indicate the extent of gene repression and induction respectively (n = 3). Blocks of squares show the level of gene expression in the leaves and roots of nine different grapevine genotypes (c) for each gene studied

Mentions: The transcript abundance of 12 ABA-related genes was studied in non-stressed (Fig. 5a) and water-stressed plants (Fig. 5b). The heat map for non-stressed plants presents the level of expression normalised for each gene by the lowest expression either in leaves or roots. The heat map for water-stressed plants presents the ratio of the average expression at day 4 to the average expression at day 1 for each genotype and tissue. Results are expressed in log2 (Fold-change relative to day 1 expression). Average expression data per genotype and water treatment, and results of ANOVA analyses are given in Additional files 4, 5 and 6.Fig. 5


ABA-mediated responses to water deficit separate grapevine genotypes by their genetic background.

Rossdeutsch L, Edwards E, Cookson SJ, Barrieu F, Gambetta GA, Delrot S, Ollat N - BMC Plant Biol. (2016)

Heatmaps of the abundance of transcripts for studied genes and their variations with water deficit. The abundance of transcripts for the genes associated with abscisic acid was recorded in the leaves and roots of nine grapevine genotypes during a water-deficit treatment. Transcript abundance at day 1 after withholding irrigation (non-stressed plants) (a), green shade indicates the level of expression relative to the lowest value (n = 3). Transcript abundance changes from day 1 to day 4 after withholding irrigation (water-stressed plants) (b), the blue and red shades indicate the extent of gene repression and induction respectively (n = 3). Blocks of squares show the level of gene expression in the leaves and roots of nine different grapevine genotypes (c) for each gene studied
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4836075&req=5

Fig5: Heatmaps of the abundance of transcripts for studied genes and their variations with water deficit. The abundance of transcripts for the genes associated with abscisic acid was recorded in the leaves and roots of nine grapevine genotypes during a water-deficit treatment. Transcript abundance at day 1 after withholding irrigation (non-stressed plants) (a), green shade indicates the level of expression relative to the lowest value (n = 3). Transcript abundance changes from day 1 to day 4 after withholding irrigation (water-stressed plants) (b), the blue and red shades indicate the extent of gene repression and induction respectively (n = 3). Blocks of squares show the level of gene expression in the leaves and roots of nine different grapevine genotypes (c) for each gene studied
Mentions: The transcript abundance of 12 ABA-related genes was studied in non-stressed (Fig. 5a) and water-stressed plants (Fig. 5b). The heat map for non-stressed plants presents the level of expression normalised for each gene by the lowest expression either in leaves or roots. The heat map for water-stressed plants presents the ratio of the average expression at day 4 to the average expression at day 1 for each genotype and tissue. Results are expressed in log2 (Fold-change relative to day 1 expression). Average expression data per genotype and water treatment, and results of ANOVA analyses are given in Additional files 4, 5 and 6.Fig. 5

Bottom Line: The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses.In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

View Article: PubMed Central - PubMed

Affiliation: UMR EGFV, ISVV-INRA, 210 chemin de Leysotte, 33882, Villenave d'Ornon, France.

ABSTRACT

Background: ABA-mediated processes are involved in plant responses to water deficit, especially the control of stomatal opening. However in grapevine it is not known if these processes participate in the phenotypic variation in drought adaptation existing between genotypes. To elucidate this question, the response to short-term water-deficit was analysed in roots and shoots of nine Vitis genotypes differing in their drought adaptation in the field. The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.

Results: Although transpiration and ABA responses were well-conserved among the genotypes, multifactorial analyses separated Vitis vinifera varieties and V. berlandieri x V. rupestris hybrids (all considered drought tolerant) from the other genotypes studied. Generally, V. vinifera varieties, followed by V. berlandieri x V. rupestris hybrids, displayed more pronounced responses to water-deficit in comparison to the other genotypes. However, changes in transcript abundance in roots were more pronounced for Vitis hybrids than V. vinifera genotypes. Changes in the expression of the cornerstone ABA biosynthetic gene VviNCED1, and the ABA transcriptional regulator VviABF1, were associated with the response of V. vinifera genotypes, while changes in VviNCED2 abundance were associated with the response of other Vitis genotypes. In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses. Interestingly, the expression of VviSnRK2.6 (an AtOST1 ortholog) was constitutively lower in roots and leaves of V. vinifera genotypes and higher in roots of V. berlandieri x V. rupestris hybrids.

Conclusions: This study highlights that Vitis genotypes exhibiting different levels of drought adaptation differ in key steps involved in ABA metabolism and signalling; both under well-watered conditions and in response to water-deficit. In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

No MeSH data available.


Related in: MedlinePlus