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ABA-mediated responses to water deficit separate grapevine genotypes by their genetic background.

Rossdeutsch L, Edwards E, Cookson SJ, Barrieu F, Gambetta GA, Delrot S, Ollat N - BMC Plant Biol. (2016)

Bottom Line: The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses.In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

View Article: PubMed Central - PubMed

Affiliation: UMR EGFV, ISVV-INRA, 210 chemin de Leysotte, 33882, Villenave d'Ornon, France.

ABSTRACT

Background: ABA-mediated processes are involved in plant responses to water deficit, especially the control of stomatal opening. However in grapevine it is not known if these processes participate in the phenotypic variation in drought adaptation existing between genotypes. To elucidate this question, the response to short-term water-deficit was analysed in roots and shoots of nine Vitis genotypes differing in their drought adaptation in the field. The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.

Results: Although transpiration and ABA responses were well-conserved among the genotypes, multifactorial analyses separated Vitis vinifera varieties and V. berlandieri x V. rupestris hybrids (all considered drought tolerant) from the other genotypes studied. Generally, V. vinifera varieties, followed by V. berlandieri x V. rupestris hybrids, displayed more pronounced responses to water-deficit in comparison to the other genotypes. However, changes in transcript abundance in roots were more pronounced for Vitis hybrids than V. vinifera genotypes. Changes in the expression of the cornerstone ABA biosynthetic gene VviNCED1, and the ABA transcriptional regulator VviABF1, were associated with the response of V. vinifera genotypes, while changes in VviNCED2 abundance were associated with the response of other Vitis genotypes. In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses. Interestingly, the expression of VviSnRK2.6 (an AtOST1 ortholog) was constitutively lower in roots and leaves of V. vinifera genotypes and higher in roots of V. berlandieri x V. rupestris hybrids.

Conclusions: This study highlights that Vitis genotypes exhibiting different levels of drought adaptation differ in key steps involved in ABA metabolism and signalling; both under well-watered conditions and in response to water-deficit. In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

No MeSH data available.


Related in: MedlinePlus

Relationship between ABA concentration changes and plant water status. Plots of the changes from day 1 to day 4 after withholding irrigation in abscisic acid (ABA) concentration in the shoot sap and transpiration (a) and shoot water potential (b) for nine grapevine genotypes (key to symbols as shown for Fig. 1c). Each point represents difference between means at day 4 and at day 1 (n = 3). The black lines show the global linear regression for all nine genotypes, dashed black lines show the 95 % interval of confidences for the regressions
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Fig4: Relationship between ABA concentration changes and plant water status. Plots of the changes from day 1 to day 4 after withholding irrigation in abscisic acid (ABA) concentration in the shoot sap and transpiration (a) and shoot water potential (b) for nine grapevine genotypes (key to symbols as shown for Fig. 1c). Each point represents difference between means at day 4 and at day 1 (n = 3). The black lines show the global linear regression for all nine genotypes, dashed black lines show the 95 % interval of confidences for the regressions

Mentions: From day 1 to day 4 without irrigation, the changes in [ABA] in shoot sap were highly correlated to changes in transpiration (Fig. 4a, R2 = 0.86, p < 0.01) and pre-dawn shoot water potential (Fig. 4b, R2 = 0.80, p < 0.01) across all the genotypes. Similar results were obtained for roots (data not shown). Several genotypes were situated outside of the confidence intervals of the regressions. Grenache had the largest difference in both [ABA] and transpiration, while 140Ru had much smaller differences in [ABA] with similarly large reduction in transpiration. The genotype 101-14Mgt was also an outlier in the relationship between change in [ABA] and shoot water potential, showing a much smaller increase of [ABA] in relation to the decrease in pre-dawn shoot water potential.Fig. 4


ABA-mediated responses to water deficit separate grapevine genotypes by their genetic background.

Rossdeutsch L, Edwards E, Cookson SJ, Barrieu F, Gambetta GA, Delrot S, Ollat N - BMC Plant Biol. (2016)

Relationship between ABA concentration changes and plant water status. Plots of the changes from day 1 to day 4 after withholding irrigation in abscisic acid (ABA) concentration in the shoot sap and transpiration (a) and shoot water potential (b) for nine grapevine genotypes (key to symbols as shown for Fig. 1c). Each point represents difference between means at day 4 and at day 1 (n = 3). The black lines show the global linear regression for all nine genotypes, dashed black lines show the 95 % interval of confidences for the regressions
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4836075&req=5

Fig4: Relationship between ABA concentration changes and plant water status. Plots of the changes from day 1 to day 4 after withholding irrigation in abscisic acid (ABA) concentration in the shoot sap and transpiration (a) and shoot water potential (b) for nine grapevine genotypes (key to symbols as shown for Fig. 1c). Each point represents difference between means at day 4 and at day 1 (n = 3). The black lines show the global linear regression for all nine genotypes, dashed black lines show the 95 % interval of confidences for the regressions
Mentions: From day 1 to day 4 without irrigation, the changes in [ABA] in shoot sap were highly correlated to changes in transpiration (Fig. 4a, R2 = 0.86, p < 0.01) and pre-dawn shoot water potential (Fig. 4b, R2 = 0.80, p < 0.01) across all the genotypes. Similar results were obtained for roots (data not shown). Several genotypes were situated outside of the confidence intervals of the regressions. Grenache had the largest difference in both [ABA] and transpiration, while 140Ru had much smaller differences in [ABA] with similarly large reduction in transpiration. The genotype 101-14Mgt was also an outlier in the relationship between change in [ABA] and shoot water potential, showing a much smaller increase of [ABA] in relation to the decrease in pre-dawn shoot water potential.Fig. 4

Bottom Line: The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses.In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

View Article: PubMed Central - PubMed

Affiliation: UMR EGFV, ISVV-INRA, 210 chemin de Leysotte, 33882, Villenave d'Ornon, France.

ABSTRACT

Background: ABA-mediated processes are involved in plant responses to water deficit, especially the control of stomatal opening. However in grapevine it is not known if these processes participate in the phenotypic variation in drought adaptation existing between genotypes. To elucidate this question, the response to short-term water-deficit was analysed in roots and shoots of nine Vitis genotypes differing in their drought adaptation in the field. The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.

Results: Although transpiration and ABA responses were well-conserved among the genotypes, multifactorial analyses separated Vitis vinifera varieties and V. berlandieri x V. rupestris hybrids (all considered drought tolerant) from the other genotypes studied. Generally, V. vinifera varieties, followed by V. berlandieri x V. rupestris hybrids, displayed more pronounced responses to water-deficit in comparison to the other genotypes. However, changes in transcript abundance in roots were more pronounced for Vitis hybrids than V. vinifera genotypes. Changes in the expression of the cornerstone ABA biosynthetic gene VviNCED1, and the ABA transcriptional regulator VviABF1, were associated with the response of V. vinifera genotypes, while changes in VviNCED2 abundance were associated with the response of other Vitis genotypes. In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses. Interestingly, the expression of VviSnRK2.6 (an AtOST1 ortholog) was constitutively lower in roots and leaves of V. vinifera genotypes and higher in roots of V. berlandieri x V. rupestris hybrids.

Conclusions: This study highlights that Vitis genotypes exhibiting different levels of drought adaptation differ in key steps involved in ABA metabolism and signalling; both under well-watered conditions and in response to water-deficit. In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

No MeSH data available.


Related in: MedlinePlus