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ABA-mediated responses to water deficit separate grapevine genotypes by their genetic background.

Rossdeutsch L, Edwards E, Cookson SJ, Barrieu F, Gambetta GA, Delrot S, Ollat N - BMC Plant Biol. (2016)

Bottom Line: The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses.In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

View Article: PubMed Central - PubMed

Affiliation: UMR EGFV, ISVV-INRA, 210 chemin de Leysotte, 33882, Villenave d'Ornon, France.

ABSTRACT

Background: ABA-mediated processes are involved in plant responses to water deficit, especially the control of stomatal opening. However in grapevine it is not known if these processes participate in the phenotypic variation in drought adaptation existing between genotypes. To elucidate this question, the response to short-term water-deficit was analysed in roots and shoots of nine Vitis genotypes differing in their drought adaptation in the field. The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.

Results: Although transpiration and ABA responses were well-conserved among the genotypes, multifactorial analyses separated Vitis vinifera varieties and V. berlandieri x V. rupestris hybrids (all considered drought tolerant) from the other genotypes studied. Generally, V. vinifera varieties, followed by V. berlandieri x V. rupestris hybrids, displayed more pronounced responses to water-deficit in comparison to the other genotypes. However, changes in transcript abundance in roots were more pronounced for Vitis hybrids than V. vinifera genotypes. Changes in the expression of the cornerstone ABA biosynthetic gene VviNCED1, and the ABA transcriptional regulator VviABF1, were associated with the response of V. vinifera genotypes, while changes in VviNCED2 abundance were associated with the response of other Vitis genotypes. In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses. Interestingly, the expression of VviSnRK2.6 (an AtOST1 ortholog) was constitutively lower in roots and leaves of V. vinifera genotypes and higher in roots of V. berlandieri x V. rupestris hybrids.

Conclusions: This study highlights that Vitis genotypes exhibiting different levels of drought adaptation differ in key steps involved in ABA metabolism and signalling; both under well-watered conditions and in response to water-deficit. In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

No MeSH data available.


Related in: MedlinePlus

Relationships between water potential and concentration of ABA, PA, DPA in root and shoot xylem sap. ABA (a, b), phaseic acid (c, d) and dihydroxyphaseic acid (d, e) in root (a, c & e) and shoot (b, d & f) xylem sap during a four day water-deficit treatment in nine grapevine genotypes (key to symbols as shown for Fig. 1c) (n = 12). The dashed line shows the global linear regression for all nine genotypes, solid lines show those genotypes with a significantly different relationship from the global linear regression (Fischer-Snedecor test; p < 0.05). The slopes of the different regressions estimate the accumulation plasticity of the various genotypes for the different compounds. Comparisons between the slopes of general regressions obtained for shoot and root xylem sap were made using a Fischer-Snedecor-test (ABA: F = 1.76, p < 0.05; PA: F = 37.7, p < 0.001: DPA: F = 3.57, p < 0.05)
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Fig3: Relationships between water potential and concentration of ABA, PA, DPA in root and shoot xylem sap. ABA (a, b), phaseic acid (c, d) and dihydroxyphaseic acid (d, e) in root (a, c & e) and shoot (b, d & f) xylem sap during a four day water-deficit treatment in nine grapevine genotypes (key to symbols as shown for Fig. 1c) (n = 12). The dashed line shows the global linear regression for all nine genotypes, solid lines show those genotypes with a significantly different relationship from the global linear regression (Fischer-Snedecor test; p < 0.05). The slopes of the different regressions estimate the accumulation plasticity of the various genotypes for the different compounds. Comparisons between the slopes of general regressions obtained for shoot and root xylem sap were made using a Fischer-Snedecor-test (ABA: F = 1.76, p < 0.05; PA: F = 37.7, p < 0.001: DPA: F = 3.57, p < 0.05)

Mentions: The [ABA], [PA] and [DPA] increased significantly in the xylem sap of the shoots and roots while water potential decreased for all genotypes. The slope of the response curve to water potential is an estimation of the accumulation capacity. In order to compare the accumulation capacity of individual genotypes to the average accumulation capacity, the general regressions and the genotype-specific regressions, significantly different from the general regressions, are presented in Fig. 3.Fig. 3


ABA-mediated responses to water deficit separate grapevine genotypes by their genetic background.

Rossdeutsch L, Edwards E, Cookson SJ, Barrieu F, Gambetta GA, Delrot S, Ollat N - BMC Plant Biol. (2016)

Relationships between water potential and concentration of ABA, PA, DPA in root and shoot xylem sap. ABA (a, b), phaseic acid (c, d) and dihydroxyphaseic acid (d, e) in root (a, c & e) and shoot (b, d & f) xylem sap during a four day water-deficit treatment in nine grapevine genotypes (key to symbols as shown for Fig. 1c) (n = 12). The dashed line shows the global linear regression for all nine genotypes, solid lines show those genotypes with a significantly different relationship from the global linear regression (Fischer-Snedecor test; p < 0.05). The slopes of the different regressions estimate the accumulation plasticity of the various genotypes for the different compounds. Comparisons between the slopes of general regressions obtained for shoot and root xylem sap were made using a Fischer-Snedecor-test (ABA: F = 1.76, p < 0.05; PA: F = 37.7, p < 0.001: DPA: F = 3.57, p < 0.05)
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4836075&req=5

Fig3: Relationships between water potential and concentration of ABA, PA, DPA in root and shoot xylem sap. ABA (a, b), phaseic acid (c, d) and dihydroxyphaseic acid (d, e) in root (a, c & e) and shoot (b, d & f) xylem sap during a four day water-deficit treatment in nine grapevine genotypes (key to symbols as shown for Fig. 1c) (n = 12). The dashed line shows the global linear regression for all nine genotypes, solid lines show those genotypes with a significantly different relationship from the global linear regression (Fischer-Snedecor test; p < 0.05). The slopes of the different regressions estimate the accumulation plasticity of the various genotypes for the different compounds. Comparisons between the slopes of general regressions obtained for shoot and root xylem sap were made using a Fischer-Snedecor-test (ABA: F = 1.76, p < 0.05; PA: F = 37.7, p < 0.001: DPA: F = 3.57, p < 0.05)
Mentions: The [ABA], [PA] and [DPA] increased significantly in the xylem sap of the shoots and roots while water potential decreased for all genotypes. The slope of the response curve to water potential is an estimation of the accumulation capacity. In order to compare the accumulation capacity of individual genotypes to the average accumulation capacity, the general regressions and the genotype-specific regressions, significantly different from the general regressions, are presented in Fig. 3.Fig. 3

Bottom Line: The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses.In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

View Article: PubMed Central - PubMed

Affiliation: UMR EGFV, ISVV-INRA, 210 chemin de Leysotte, 33882, Villenave d'Ornon, France.

ABSTRACT

Background: ABA-mediated processes are involved in plant responses to water deficit, especially the control of stomatal opening. However in grapevine it is not known if these processes participate in the phenotypic variation in drought adaptation existing between genotypes. To elucidate this question, the response to short-term water-deficit was analysed in roots and shoots of nine Vitis genotypes differing in their drought adaptation in the field. The transcript abundance of 12 genes involved in ABA biosynthesis, catabolism, and signalling were monitored, together with physiological and metabolic parameters related to ABA and its role in controlling plant transpiration.

Results: Although transpiration and ABA responses were well-conserved among the genotypes, multifactorial analyses separated Vitis vinifera varieties and V. berlandieri x V. rupestris hybrids (all considered drought tolerant) from the other genotypes studied. Generally, V. vinifera varieties, followed by V. berlandieri x V. rupestris hybrids, displayed more pronounced responses to water-deficit in comparison to the other genotypes. However, changes in transcript abundance in roots were more pronounced for Vitis hybrids than V. vinifera genotypes. Changes in the expression of the cornerstone ABA biosynthetic gene VviNCED1, and the ABA transcriptional regulator VviABF1, were associated with the response of V. vinifera genotypes, while changes in VviNCED2 abundance were associated with the response of other Vitis genotypes. In contrast, the ABA RCAR receptors were not identified as key components of the genotypic variability of water-deficit responses. Interestingly, the expression of VviSnRK2.6 (an AtOST1 ortholog) was constitutively lower in roots and leaves of V. vinifera genotypes and higher in roots of V. berlandieri x V. rupestris hybrids.

Conclusions: This study highlights that Vitis genotypes exhibiting different levels of drought adaptation differ in key steps involved in ABA metabolism and signalling; both under well-watered conditions and in response to water-deficit. In addition, it supports that adaptation may be related to various mechanisms related or not to ABA responses.

No MeSH data available.


Related in: MedlinePlus