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Anti-diabetic activity of traditional Indian gold containing preparation: Shadguna Balijarita Makaradhwaja on streptozotocin induced diabetic rats.

Khedekar S, Rukkudin G, Ravishankar B, Prajapati P - J Intercult Ethnopharmacol (2016)

Bottom Line: The effects of treatment on body weight changes and blood glucose levels were quantified on day 1, 5, 10, 15 and 21 of the experiments.Blood sugar level of test drug group shown a significant decrease (279.11 ± 57.95) compared with diabetic rats.The present study demonstrates that SBM and dried extract of T. cordifolia with honey significantly reduces the blood glucose level and shows anti-diabetic effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Rasashastra and Bhaishajya Kalpana, Assistant Director for Post Graduate Studies, SSAM & H, Hirawadi, Maharashtra University of Health Sciences, Nashik, Maharashtra, India.

ABSTRACT

Background: Makaradhwaja a gold containing mercurial preparation used for diabetes mellitus in indigenous system of medicine. It is a popular aphrodisiac and rejuvenator traditional medicine. It is prepared by using processed gold, mercury and sulfur in different ratios by applying intermittent heating pattern in Valuka Yantra.

Objectives: The aim of the study was to evaluate anti-diabetic effect of Shadguna Balijarita Makaradhwaja (SBM) on streptozotocin (STZ) induced diabetic rats.

Materials and methods: Diabetes was induced to normal rats by injecting STZ in dose 40 mg/kg. Powdered SBM and dried extract of Tinospora cordifolia were mixed with honey and administered orally for 20 days at dose 2.63 mg/kg and 42.34 mg/kg body weight, respectively. The effects of treatment on body weight changes and blood glucose levels were quantified on day 1, 5, 10, 15 and 21 of the experiments. On the 21(st) day, animals were sacrificed and gross histopathological changes in liver, kidney and pancreas were illustrated. Blood sugar level, glyacated hemoglobin, blood urea, serum cholesterol, serum creatinine, serum triglyceride and serum protein were estimated with standard methods. The study was conducted in the year 2011.

Results: Test drug observed significant decrease (P < 0.001) in glyacated hemoglobin level compared to diabetic control rats. Blood sugar level of test drug group shown a significant decrease (279.11 ± 57.95) compared with diabetic rats.

Conclusion: The present study demonstrates that SBM and dried extract of T. cordifolia with honey significantly reduces the blood glucose level and shows anti-diabetic effect.

No MeSH data available.


Related in: MedlinePlus

(a) Normal cytoarchitecture of liver in normal control group (1 × 400 magnification), (b) Photomicrographs of representative section of Liver. Macro and micro fatty changes, cell infiltration in almost all the sections streptozotocin control group (1 × 400 magnification) (c) Shadguna Balijarita Makaradhwaja treated rats shows almost normal cytoarchitecture of liver in comparison with streptozotocin control group diabetic rats (1 × 400 magnification), (d) Glibenclamide treated rat showed almost normal cytoarchitecture of liver sections in comparison with streptozotocin control group diabetic rats (1 × 400 magnification)
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Figure 1: (a) Normal cytoarchitecture of liver in normal control group (1 × 400 magnification), (b) Photomicrographs of representative section of Liver. Macro and micro fatty changes, cell infiltration in almost all the sections streptozotocin control group (1 × 400 magnification) (c) Shadguna Balijarita Makaradhwaja treated rats shows almost normal cytoarchitecture of liver in comparison with streptozotocin control group diabetic rats (1 × 400 magnification), (d) Glibenclamide treated rat showed almost normal cytoarchitecture of liver sections in comparison with streptozotocin control group diabetic rats (1 × 400 magnification)

Mentions: The accumulation of fat in liver was observed in histopathological study. SBM and RS treated animals restored the histological changes [Figure 1a-d]. Inflammation in blood vessels, increase in the thickness of bowman capsules, fat deposition, and change in size of the glomerulus were found in the kidney of diabetic rats. The treatment with SBM showed the normal histopathology of the kidney without any inflammatory vessels and fat deposition [Figure 2a-d]. Decreased Islets of Langerhans, smashed size of β cells and extensive necrosis, fibrosis and atrophy were observed in the pancreas of diabetic rats. STZ induced diabetic rat treated with SBM and glibenclamide restored the necrotic and fibrotic changes and up to moderate levels, raised the number of β cells. [Figure 3a-d].


Anti-diabetic activity of traditional Indian gold containing preparation: Shadguna Balijarita Makaradhwaja on streptozotocin induced diabetic rats.

Khedekar S, Rukkudin G, Ravishankar B, Prajapati P - J Intercult Ethnopharmacol (2016)

(a) Normal cytoarchitecture of liver in normal control group (1 × 400 magnification), (b) Photomicrographs of representative section of Liver. Macro and micro fatty changes, cell infiltration in almost all the sections streptozotocin control group (1 × 400 magnification) (c) Shadguna Balijarita Makaradhwaja treated rats shows almost normal cytoarchitecture of liver in comparison with streptozotocin control group diabetic rats (1 × 400 magnification), (d) Glibenclamide treated rat showed almost normal cytoarchitecture of liver sections in comparison with streptozotocin control group diabetic rats (1 × 400 magnification)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835991&req=5

Figure 1: (a) Normal cytoarchitecture of liver in normal control group (1 × 400 magnification), (b) Photomicrographs of representative section of Liver. Macro and micro fatty changes, cell infiltration in almost all the sections streptozotocin control group (1 × 400 magnification) (c) Shadguna Balijarita Makaradhwaja treated rats shows almost normal cytoarchitecture of liver in comparison with streptozotocin control group diabetic rats (1 × 400 magnification), (d) Glibenclamide treated rat showed almost normal cytoarchitecture of liver sections in comparison with streptozotocin control group diabetic rats (1 × 400 magnification)
Mentions: The accumulation of fat in liver was observed in histopathological study. SBM and RS treated animals restored the histological changes [Figure 1a-d]. Inflammation in blood vessels, increase in the thickness of bowman capsules, fat deposition, and change in size of the glomerulus were found in the kidney of diabetic rats. The treatment with SBM showed the normal histopathology of the kidney without any inflammatory vessels and fat deposition [Figure 2a-d]. Decreased Islets of Langerhans, smashed size of β cells and extensive necrosis, fibrosis and atrophy were observed in the pancreas of diabetic rats. STZ induced diabetic rat treated with SBM and glibenclamide restored the necrotic and fibrotic changes and up to moderate levels, raised the number of β cells. [Figure 3a-d].

Bottom Line: The effects of treatment on body weight changes and blood glucose levels were quantified on day 1, 5, 10, 15 and 21 of the experiments.Blood sugar level of test drug group shown a significant decrease (279.11 ± 57.95) compared with diabetic rats.The present study demonstrates that SBM and dried extract of T. cordifolia with honey significantly reduces the blood glucose level and shows anti-diabetic effect.

View Article: PubMed Central - PubMed

Affiliation: Department of Rasashastra and Bhaishajya Kalpana, Assistant Director for Post Graduate Studies, SSAM & H, Hirawadi, Maharashtra University of Health Sciences, Nashik, Maharashtra, India.

ABSTRACT

Background: Makaradhwaja a gold containing mercurial preparation used for diabetes mellitus in indigenous system of medicine. It is a popular aphrodisiac and rejuvenator traditional medicine. It is prepared by using processed gold, mercury and sulfur in different ratios by applying intermittent heating pattern in Valuka Yantra.

Objectives: The aim of the study was to evaluate anti-diabetic effect of Shadguna Balijarita Makaradhwaja (SBM) on streptozotocin (STZ) induced diabetic rats.

Materials and methods: Diabetes was induced to normal rats by injecting STZ in dose 40 mg/kg. Powdered SBM and dried extract of Tinospora cordifolia were mixed with honey and administered orally for 20 days at dose 2.63 mg/kg and 42.34 mg/kg body weight, respectively. The effects of treatment on body weight changes and blood glucose levels were quantified on day 1, 5, 10, 15 and 21 of the experiments. On the 21(st) day, animals were sacrificed and gross histopathological changes in liver, kidney and pancreas were illustrated. Blood sugar level, glyacated hemoglobin, blood urea, serum cholesterol, serum creatinine, serum triglyceride and serum protein were estimated with standard methods. The study was conducted in the year 2011.

Results: Test drug observed significant decrease (P < 0.001) in glyacated hemoglobin level compared to diabetic control rats. Blood sugar level of test drug group shown a significant decrease (279.11 ± 57.95) compared with diabetic rats.

Conclusion: The present study demonstrates that SBM and dried extract of T. cordifolia with honey significantly reduces the blood glucose level and shows anti-diabetic effect.

No MeSH data available.


Related in: MedlinePlus