Limits...
Anticoagulant effect and safety assessment of an aqueous extract of Pseudocedrela kotschyi (Schweinf.) harms and Adenia cissampeloides (Planch. Ex Hook.) harms.

Nyansah WB, Koffuor GA, Asare F, Gyanfosu L - J Intercult Ethnopharmacol (2016)

Bottom Line: The PAE treatment resulted in a significant increase (P ≤ 0.05-0.0001) in BT and CT in vivo compared with control.The no-observed-adverse-effect-level was <2000 mg/kg when given orally.PAE has anticoagulant effect in vitro and is safe to use at oral doses <2000 mg/kg.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

ABSTRACT

Background: Currently available therapeutic options for thromboembolic disorders are often very expensive and are associated with unfavorable side effects.

Aim: To establish the anticoagulant effect and safety profile of an extract made from of the root bark of Pseudocedrela kotschyi (Schweinf.) Harms and the aerial part of Adenia cissampeloides (Planch. ex Hook.) Harms (PAE).

Materials and methods: PAE (0.5-2.0 g/L) effect on prothrombin time (PT) and activated partial thromboplastin time (aPTT) were evaluated on whole blood drawn from the marginal ear vein of New Zealand White rabbits. Effect of PAE (250-2000 mg/kg) on bleeding time (BT) and clotting time (CT) in Sprague-Dawley rats were also assessed. Histopathological, hematological, and liver function studies were also carried out to assess the safety for use of PAE (250-2000 mg/kg).

Results: PAE had no significant effect (P > 0.05) on PT, but resulted in a significant increase (P ≤ 0.05-0.0001) in aPTT. The PAE treatment resulted in a significant increase (P ≤ 0.05-0.0001) in BT and CT in vivo compared with control. Safety studies indicated no deaths with PAE treatment with hematological and liver function tests being normal. Histological studies revealed pathological changes in the liver at a PAE treatment dose of 2000 mg/kg but all doses had no detrimental effect on kidney and stomach tissue. The no-observed-adverse-effect-level was <2000 mg/kg when given orally.

Conclusion: PAE has anticoagulant effect in vitro and is safe to use at oral doses <2000 mg/kg.

No MeSH data available.


Related in: MedlinePlus

The effect of PAE and Aspirin on bleeding time in Sprague-Dawley rats. Values plotted are means ± standard error of the mean; (n = 5). ****P ≤ 0.0001; ns P > 0.05 compared to control (One-way ANOVA followed by Sidak’s post-hoc test). ††††P ≤ 0.0001; ns P > 0.05 comparison between aspirin and the various doses of Pseudocedrela kotschyi and Adenia cissampeloides extract
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4835990&req=5

Figure 4: The effect of PAE and Aspirin on bleeding time in Sprague-Dawley rats. Values plotted are means ± standard error of the mean; (n = 5). ****P ≤ 0.0001; ns P > 0.05 compared to control (One-way ANOVA followed by Sidak’s post-hoc test). ††††P ≤ 0.0001; ns P > 0.05 comparison between aspirin and the various doses of Pseudocedrela kotschyi and Adenia cissampeloides extract

Mentions: PAE treatment (250, 500, 1000 and 2000 mg/kg) showed a dose-dependent increase (P ≤ 0.0001) in bleeding and CT compared to the control. There were dose-dependent significant increases (P ≤ 0.0001) in bleeding and CT between PAE and aspirin treatments [Figures 4 and 5]. There was a significant increase (P ≤ 0.0001) in CT as compared with BT in both aspirin and PAE treated groups. This difference between bleeding and CT was significantly higher (P ≤ 0.0001) in the treatment groups compared with the untreated group (control) [Figure 6].


Anticoagulant effect and safety assessment of an aqueous extract of Pseudocedrela kotschyi (Schweinf.) harms and Adenia cissampeloides (Planch. Ex Hook.) harms.

Nyansah WB, Koffuor GA, Asare F, Gyanfosu L - J Intercult Ethnopharmacol (2016)

The effect of PAE and Aspirin on bleeding time in Sprague-Dawley rats. Values plotted are means ± standard error of the mean; (n = 5). ****P ≤ 0.0001; ns P > 0.05 compared to control (One-way ANOVA followed by Sidak’s post-hoc test). ††††P ≤ 0.0001; ns P > 0.05 comparison between aspirin and the various doses of Pseudocedrela kotschyi and Adenia cissampeloides extract
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835990&req=5

Figure 4: The effect of PAE and Aspirin on bleeding time in Sprague-Dawley rats. Values plotted are means ± standard error of the mean; (n = 5). ****P ≤ 0.0001; ns P > 0.05 compared to control (One-way ANOVA followed by Sidak’s post-hoc test). ††††P ≤ 0.0001; ns P > 0.05 comparison between aspirin and the various doses of Pseudocedrela kotschyi and Adenia cissampeloides extract
Mentions: PAE treatment (250, 500, 1000 and 2000 mg/kg) showed a dose-dependent increase (P ≤ 0.0001) in bleeding and CT compared to the control. There were dose-dependent significant increases (P ≤ 0.0001) in bleeding and CT between PAE and aspirin treatments [Figures 4 and 5]. There was a significant increase (P ≤ 0.0001) in CT as compared with BT in both aspirin and PAE treated groups. This difference between bleeding and CT was significantly higher (P ≤ 0.0001) in the treatment groups compared with the untreated group (control) [Figure 6].

Bottom Line: The PAE treatment resulted in a significant increase (P ≤ 0.05-0.0001) in BT and CT in vivo compared with control.The no-observed-adverse-effect-level was <2000 mg/kg when given orally.PAE has anticoagulant effect in vitro and is safe to use at oral doses <2000 mg/kg.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

ABSTRACT

Background: Currently available therapeutic options for thromboembolic disorders are often very expensive and are associated with unfavorable side effects.

Aim: To establish the anticoagulant effect and safety profile of an extract made from of the root bark of Pseudocedrela kotschyi (Schweinf.) Harms and the aerial part of Adenia cissampeloides (Planch. ex Hook.) Harms (PAE).

Materials and methods: PAE (0.5-2.0 g/L) effect on prothrombin time (PT) and activated partial thromboplastin time (aPTT) were evaluated on whole blood drawn from the marginal ear vein of New Zealand White rabbits. Effect of PAE (250-2000 mg/kg) on bleeding time (BT) and clotting time (CT) in Sprague-Dawley rats were also assessed. Histopathological, hematological, and liver function studies were also carried out to assess the safety for use of PAE (250-2000 mg/kg).

Results: PAE had no significant effect (P > 0.05) on PT, but resulted in a significant increase (P ≤ 0.05-0.0001) in aPTT. The PAE treatment resulted in a significant increase (P ≤ 0.05-0.0001) in BT and CT in vivo compared with control. Safety studies indicated no deaths with PAE treatment with hematological and liver function tests being normal. Histological studies revealed pathological changes in the liver at a PAE treatment dose of 2000 mg/kg but all doses had no detrimental effect on kidney and stomach tissue. The no-observed-adverse-effect-level was <2000 mg/kg when given orally.

Conclusion: PAE has anticoagulant effect in vitro and is safe to use at oral doses <2000 mg/kg.

No MeSH data available.


Related in: MedlinePlus