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Methanolic leaf extract of Gymnema sylvestre augments glucose uptake and ameliorates insulin resistance by upregulating glucose transporter-4, peroxisome proliferator-activated receptor-gamma, adiponectin, and leptin levels in vitro.

Kumar PM, Venkataranganna MV, Manjunath K, Viswanatha GL, Ashok G - J Intercult Ethnopharmacol (2016)

Bottom Line: The findings of the experiments have demonstrated a significant and dose-dependent increase in glucose uptake in all the tested concentrations of MLGS, further the glucose uptake activity of MLGS (750 μg/ml) was at par with rosiglitazone (50 μg/ml).Concomitantly, MLGS has shown enhanced GLUT-4 and PPAR-γ gene expressions in L6 myotubes.Furthermore, cycloheximide (CHX) had completely abolished the glucose uptake activity of MLGS when co-incubated, which further confirmed that glucose uptake activity of MLGS was linked to enhanced expression of GLUT-4 and PPAR-γ.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Padmashree Institute of Information Sciences, Bengaluru, Karnataka, India.

ABSTRACT

Aims: The present study was undertaken to evaluate the effect of methanolic leaf extract of Gymnema sylvestre (MLGS) on glucose transport (GLUT) and insulin resistance in vitro.

Materials and methods: Peroxisome proliferator-activated receptor-gamma (PPAR-γ) and GLUT-4 expression were assessed in L6 myotubes for concluding the GLUT activity, and adiponectin and leptin expression was studied in 3T3 L1 murine adipocyte cell line to determine the effect of MLGS (250-750 μg/ml) on insulin resistance.

Results: The findings of the experiments have demonstrated a significant and dose-dependent increase in glucose uptake in all the tested concentrations of MLGS, further the glucose uptake activity of MLGS (750 μg/ml) was at par with rosiglitazone (50 μg/ml). Concomitantly, MLGS has shown enhanced GLUT-4 and PPAR-γ gene expressions in L6 myotubes. Furthermore, cycloheximide (CHX) had completely abolished the glucose uptake activity of MLGS when co-incubated, which further confirmed that glucose uptake activity of MLGS was linked to enhanced expression of GLUT-4 and PPAR-γ. In addition, in another experimental set, MLGS showed enhanced expression of adiponectin and leptin, thus confirms the ameliorative effect of MLGS on insulin resistance.

Conclusion: These findings suggest that MLGS has an enhanced glucose uptake activity in L6 myotubes, and ameliorate the insulin resistance in 3T3 L1 murine adipocyte cell line in vitro.

No MeSH data available.


Related in: MedlinePlus

Effect of the methanolic leaf extract of Gymnema sylvestre (MLGS) on Adiponectin transcripts in 3T3 L1 cell lines. (a and b) - M: Ikbp marker, Lane 1: Control, Lane 2: 50 μg/ml rosiglitazone, Lane 3: 250 μg/ml MLGS, Lane 4: 500 μg/ml MLGS, Lane 5: 750 μg/ml MLGS, (c) The values are x ± standard error of mean (n = 3) of independent experiments, means of various groups were statistically compared by ANOVA followed by Tukey’s multiple comparison test using Graph Pad version 4.0. †P < 0.001 corresponds to rosiglitazone versus control; *P < 0.05, **P < 0.01, ***< 0.01 corresponds to MLGS versus control
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Figure 5: Effect of the methanolic leaf extract of Gymnema sylvestre (MLGS) on Adiponectin transcripts in 3T3 L1 cell lines. (a and b) - M: Ikbp marker, Lane 1: Control, Lane 2: 50 μg/ml rosiglitazone, Lane 3: 250 μg/ml MLGS, Lane 4: 500 μg/ml MLGS, Lane 5: 750 μg/ml MLGS, (c) The values are x ± standard error of mean (n = 3) of independent experiments, means of various groups were statistically compared by ANOVA followed by Tukey’s multiple comparison test using Graph Pad version 4.0. †P < 0.001 corresponds to rosiglitazone versus control; *P < 0.05, **P < 0.01, ***< 0.01 corresponds to MLGS versus control

Mentions: Based on the above findings, it was concluded that MLGS could upregulate the expression of GLUT-4 and PPAR-γ, and thereby increased the glucose uptake in vitro. Further, the glucose uptake activity of MLGS was completely abolished in the presence of CHX (1 μg/ml), which is a protein synthesis inhibitor, thus it was confirmed that glucose uptake activity of MLGS is mediating through enhanced expression of GLUT-4 and PPAR-γ. Furthermore, in another experimental set, MLGS has shown enhanced expression of adiponectin, leptin by 3.9-fold and 7.1-fold, respectively, compared to control [Figures 5a-c and 6a-c] Collectively, it can be concluded that MLGS shows hypoglycemic activity through enhanced expression of GLUT-4, and reverses insulin resistance by enhancing the expression of PPAR-γ, adiponectin, and leptin, respectively.


Methanolic leaf extract of Gymnema sylvestre augments glucose uptake and ameliorates insulin resistance by upregulating glucose transporter-4, peroxisome proliferator-activated receptor-gamma, adiponectin, and leptin levels in vitro.

Kumar PM, Venkataranganna MV, Manjunath K, Viswanatha GL, Ashok G - J Intercult Ethnopharmacol (2016)

Effect of the methanolic leaf extract of Gymnema sylvestre (MLGS) on Adiponectin transcripts in 3T3 L1 cell lines. (a and b) - M: Ikbp marker, Lane 1: Control, Lane 2: 50 μg/ml rosiglitazone, Lane 3: 250 μg/ml MLGS, Lane 4: 500 μg/ml MLGS, Lane 5: 750 μg/ml MLGS, (c) The values are x ± standard error of mean (n = 3) of independent experiments, means of various groups were statistically compared by ANOVA followed by Tukey’s multiple comparison test using Graph Pad version 4.0. †P < 0.001 corresponds to rosiglitazone versus control; *P < 0.05, **P < 0.01, ***< 0.01 corresponds to MLGS versus control
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835989&req=5

Figure 5: Effect of the methanolic leaf extract of Gymnema sylvestre (MLGS) on Adiponectin transcripts in 3T3 L1 cell lines. (a and b) - M: Ikbp marker, Lane 1: Control, Lane 2: 50 μg/ml rosiglitazone, Lane 3: 250 μg/ml MLGS, Lane 4: 500 μg/ml MLGS, Lane 5: 750 μg/ml MLGS, (c) The values are x ± standard error of mean (n = 3) of independent experiments, means of various groups were statistically compared by ANOVA followed by Tukey’s multiple comparison test using Graph Pad version 4.0. †P < 0.001 corresponds to rosiglitazone versus control; *P < 0.05, **P < 0.01, ***< 0.01 corresponds to MLGS versus control
Mentions: Based on the above findings, it was concluded that MLGS could upregulate the expression of GLUT-4 and PPAR-γ, and thereby increased the glucose uptake in vitro. Further, the glucose uptake activity of MLGS was completely abolished in the presence of CHX (1 μg/ml), which is a protein synthesis inhibitor, thus it was confirmed that glucose uptake activity of MLGS is mediating through enhanced expression of GLUT-4 and PPAR-γ. Furthermore, in another experimental set, MLGS has shown enhanced expression of adiponectin, leptin by 3.9-fold and 7.1-fold, respectively, compared to control [Figures 5a-c and 6a-c] Collectively, it can be concluded that MLGS shows hypoglycemic activity through enhanced expression of GLUT-4, and reverses insulin resistance by enhancing the expression of PPAR-γ, adiponectin, and leptin, respectively.

Bottom Line: The findings of the experiments have demonstrated a significant and dose-dependent increase in glucose uptake in all the tested concentrations of MLGS, further the glucose uptake activity of MLGS (750 μg/ml) was at par with rosiglitazone (50 μg/ml).Concomitantly, MLGS has shown enhanced GLUT-4 and PPAR-γ gene expressions in L6 myotubes.Furthermore, cycloheximide (CHX) had completely abolished the glucose uptake activity of MLGS when co-incubated, which further confirmed that glucose uptake activity of MLGS was linked to enhanced expression of GLUT-4 and PPAR-γ.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, Padmashree Institute of Information Sciences, Bengaluru, Karnataka, India.

ABSTRACT

Aims: The present study was undertaken to evaluate the effect of methanolic leaf extract of Gymnema sylvestre (MLGS) on glucose transport (GLUT) and insulin resistance in vitro.

Materials and methods: Peroxisome proliferator-activated receptor-gamma (PPAR-γ) and GLUT-4 expression were assessed in L6 myotubes for concluding the GLUT activity, and adiponectin and leptin expression was studied in 3T3 L1 murine adipocyte cell line to determine the effect of MLGS (250-750 μg/ml) on insulin resistance.

Results: The findings of the experiments have demonstrated a significant and dose-dependent increase in glucose uptake in all the tested concentrations of MLGS, further the glucose uptake activity of MLGS (750 μg/ml) was at par with rosiglitazone (50 μg/ml). Concomitantly, MLGS has shown enhanced GLUT-4 and PPAR-γ gene expressions in L6 myotubes. Furthermore, cycloheximide (CHX) had completely abolished the glucose uptake activity of MLGS when co-incubated, which further confirmed that glucose uptake activity of MLGS was linked to enhanced expression of GLUT-4 and PPAR-γ. In addition, in another experimental set, MLGS showed enhanced expression of adiponectin and leptin, thus confirms the ameliorative effect of MLGS on insulin resistance.

Conclusion: These findings suggest that MLGS has an enhanced glucose uptake activity in L6 myotubes, and ameliorate the insulin resistance in 3T3 L1 murine adipocyte cell line in vitro.

No MeSH data available.


Related in: MedlinePlus