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Imbalance of the antioxidative system by plumbagin and Plumbago indica L. extract induces hepatotoxicity in mice.

Sukkasem N, Chatuphonprasert W, Tatiya-Aphiradee N, Jarukamjorn K - J Intercult Ethnopharmacol (2016)

Bottom Line: Plasma alanine transaminase (ALT) and aspartate transaminase (AST) levels, hepatic lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, and the ratio of reduced to oxidized glutathione (GSH/GSSG) were determined.Plumbagin and PI significantly increased plasma ALT and AST levels, hepatic lipid peroxidation, and GPx activity but significantly decreased hepatic SOD and CAT activities.The GSH/GSSG ratio was significantly reduced by plumbagin.

View Article: PubMed Central - PubMed

Affiliation: Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology, Khon Kaen University.

ABSTRACT

Background/aim: Plumbago indica (PI) L. and its active constituent, plumbagin, has been traditionally claimed for several pharmacological activities; however, there is little information regarding their toxicity. The present study aims to examine the effects of plumbagin and PI extract (PI) on hepatic histomorphology and antioxidative system in mice.

Materials and methods: Adult male intelligent character recognition mice were intragastrically administered plumbagin (1, 5, and 15 mg/kg/day) or PI (20, 200, and 1,000 mg/kg/day) consecutively for 14 days. Hepatic histomorphology was examined. Plasma alanine transaminase (ALT) and aspartate transaminase (AST) levels, hepatic lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, and the ratio of reduced to oxidized glutathione (GSH/GSSG) were determined.

Results: Plumbagin and PI concentration-dependently induced hepatic injury based on histopathological changes via imbalance of antioxidative system. Plumbagin and PI significantly increased plasma ALT and AST levels, hepatic lipid peroxidation, and GPx activity but significantly decreased hepatic SOD and CAT activities. The GSH/GSSG ratio was significantly reduced by plumbagin.

Conclusion: Plumbagin and PI caused hepatotoxic effects in the mice by unbalancing of the redox defense system. Therefore, plumbagin and PI-containing supplements should be used cautiously, especially when consumed in high quantities or for long periods.

No MeSH data available.


Related in: MedlinePlus

The superoxide dismutase (SOD) activity in the mouse livers after the treatments with plumbagin and the Plumbago indica extract. Mice were treated, and the SOD activity was measured as described in the method (n = 5). *P<0.05, **P<0.01, ***P<0.001 versus control using one-way ANOVA with least significant different post hoc test
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Figure 5: The superoxide dismutase (SOD) activity in the mouse livers after the treatments with plumbagin and the Plumbago indica extract. Mice were treated, and the SOD activity was measured as described in the method (n = 5). *P<0.05, **P<0.01, ***P<0.001 versus control using one-way ANOVA with least significant different post hoc test

Mentions: Both of plumbagin and the PI extract significantly lowered SOD activity in the mouse livers to at least 2 times lesser than the control group [Figure 5]. The CAT activity was significantly decreased by both plumbagin and the PI extract in a dose-dependent pattern [Figure 6].


Imbalance of the antioxidative system by plumbagin and Plumbago indica L. extract induces hepatotoxicity in mice.

Sukkasem N, Chatuphonprasert W, Tatiya-Aphiradee N, Jarukamjorn K - J Intercult Ethnopharmacol (2016)

The superoxide dismutase (SOD) activity in the mouse livers after the treatments with plumbagin and the Plumbago indica extract. Mice were treated, and the SOD activity was measured as described in the method (n = 5). *P<0.05, **P<0.01, ***P<0.001 versus control using one-way ANOVA with least significant different post hoc test
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835988&req=5

Figure 5: The superoxide dismutase (SOD) activity in the mouse livers after the treatments with plumbagin and the Plumbago indica extract. Mice were treated, and the SOD activity was measured as described in the method (n = 5). *P<0.05, **P<0.01, ***P<0.001 versus control using one-way ANOVA with least significant different post hoc test
Mentions: Both of plumbagin and the PI extract significantly lowered SOD activity in the mouse livers to at least 2 times lesser than the control group [Figure 5]. The CAT activity was significantly decreased by both plumbagin and the PI extract in a dose-dependent pattern [Figure 6].

Bottom Line: Plasma alanine transaminase (ALT) and aspartate transaminase (AST) levels, hepatic lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, and the ratio of reduced to oxidized glutathione (GSH/GSSG) were determined.Plumbagin and PI significantly increased plasma ALT and AST levels, hepatic lipid peroxidation, and GPx activity but significantly decreased hepatic SOD and CAT activities.The GSH/GSSG ratio was significantly reduced by plumbagin.

View Article: PubMed Central - PubMed

Affiliation: Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology, Khon Kaen University.

ABSTRACT

Background/aim: Plumbago indica (PI) L. and its active constituent, plumbagin, has been traditionally claimed for several pharmacological activities; however, there is little information regarding their toxicity. The present study aims to examine the effects of plumbagin and PI extract (PI) on hepatic histomorphology and antioxidative system in mice.

Materials and methods: Adult male intelligent character recognition mice were intragastrically administered plumbagin (1, 5, and 15 mg/kg/day) or PI (20, 200, and 1,000 mg/kg/day) consecutively for 14 days. Hepatic histomorphology was examined. Plasma alanine transaminase (ALT) and aspartate transaminase (AST) levels, hepatic lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, and the ratio of reduced to oxidized glutathione (GSH/GSSG) were determined.

Results: Plumbagin and PI concentration-dependently induced hepatic injury based on histopathological changes via imbalance of antioxidative system. Plumbagin and PI significantly increased plasma ALT and AST levels, hepatic lipid peroxidation, and GPx activity but significantly decreased hepatic SOD and CAT activities. The GSH/GSSG ratio was significantly reduced by plumbagin.

Conclusion: Plumbagin and PI caused hepatotoxic effects in the mice by unbalancing of the redox defense system. Therefore, plumbagin and PI-containing supplements should be used cautiously, especially when consumed in high quantities or for long periods.

No MeSH data available.


Related in: MedlinePlus