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Imbalance of the antioxidative system by plumbagin and Plumbago indica L. extract induces hepatotoxicity in mice.

Sukkasem N, Chatuphonprasert W, Tatiya-Aphiradee N, Jarukamjorn K - J Intercult Ethnopharmacol (2016)

Bottom Line: Plasma alanine transaminase (ALT) and aspartate transaminase (AST) levels, hepatic lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, and the ratio of reduced to oxidized glutathione (GSH/GSSG) were determined.Plumbagin and PI significantly increased plasma ALT and AST levels, hepatic lipid peroxidation, and GPx activity but significantly decreased hepatic SOD and CAT activities.The GSH/GSSG ratio was significantly reduced by plumbagin.

View Article: PubMed Central - PubMed

Affiliation: Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology, Khon Kaen University.

ABSTRACT

Background/aim: Plumbago indica (PI) L. and its active constituent, plumbagin, has been traditionally claimed for several pharmacological activities; however, there is little information regarding their toxicity. The present study aims to examine the effects of plumbagin and PI extract (PI) on hepatic histomorphology and antioxidative system in mice.

Materials and methods: Adult male intelligent character recognition mice were intragastrically administered plumbagin (1, 5, and 15 mg/kg/day) or PI (20, 200, and 1,000 mg/kg/day) consecutively for 14 days. Hepatic histomorphology was examined. Plasma alanine transaminase (ALT) and aspartate transaminase (AST) levels, hepatic lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, and the ratio of reduced to oxidized glutathione (GSH/GSSG) were determined.

Results: Plumbagin and PI concentration-dependently induced hepatic injury based on histopathological changes via imbalance of antioxidative system. Plumbagin and PI significantly increased plasma ALT and AST levels, hepatic lipid peroxidation, and GPx activity but significantly decreased hepatic SOD and CAT activities. The GSH/GSSG ratio was significantly reduced by plumbagin.

Conclusion: Plumbagin and PI caused hepatotoxic effects in the mice by unbalancing of the redox defense system. Therefore, plumbagin and PI-containing supplements should be used cautiously, especially when consumed in high quantities or for long periods.

No MeSH data available.


Related in: MedlinePlus

The weight profiles of mice during the treatments with plumbagin and the Plumbago indica extract. Mice were treated as described in the method (n = 5). Plum 1, 5, and 15, plumbagin in 0.5% carboxymethyl cellulose (CMC) at the doses of 1, 5, and 15 mg/kg/day, i.g., for 14 days; P. indica 20, 200, and 1,000, P. indica extract in 0.5% CMC at the doses of 20, 200, and 1,000 mg/kg/day, i.g., for 14 days
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Figure 3: The weight profiles of mice during the treatments with plumbagin and the Plumbago indica extract. Mice were treated as described in the method (n = 5). Plum 1, 5, and 15, plumbagin in 0.5% carboxymethyl cellulose (CMC) at the doses of 1, 5, and 15 mg/kg/day, i.g., for 14 days; P. indica 20, 200, and 1,000, P. indica extract in 0.5% CMC at the doses of 20, 200, and 1,000 mg/kg/day, i.g., for 14 days

Mentions: Plumbagin and the PI extract had no significant influence on the profile of mouse body weight [Figure 3]. However, the treatments with plumbagin and the PI extract significantly elevated the levels of ALT and AST, compared with the control mice [Table 1]. While plumbagin significantly increased both plasma ALT and AST levels in a dose-dependent manner, the PI extract only increased the AST levels dose-dependently; the ALT levels were increased in a dose-independent manner.


Imbalance of the antioxidative system by plumbagin and Plumbago indica L. extract induces hepatotoxicity in mice.

Sukkasem N, Chatuphonprasert W, Tatiya-Aphiradee N, Jarukamjorn K - J Intercult Ethnopharmacol (2016)

The weight profiles of mice during the treatments with plumbagin and the Plumbago indica extract. Mice were treated as described in the method (n = 5). Plum 1, 5, and 15, plumbagin in 0.5% carboxymethyl cellulose (CMC) at the doses of 1, 5, and 15 mg/kg/day, i.g., for 14 days; P. indica 20, 200, and 1,000, P. indica extract in 0.5% CMC at the doses of 20, 200, and 1,000 mg/kg/day, i.g., for 14 days
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835988&req=5

Figure 3: The weight profiles of mice during the treatments with plumbagin and the Plumbago indica extract. Mice were treated as described in the method (n = 5). Plum 1, 5, and 15, plumbagin in 0.5% carboxymethyl cellulose (CMC) at the doses of 1, 5, and 15 mg/kg/day, i.g., for 14 days; P. indica 20, 200, and 1,000, P. indica extract in 0.5% CMC at the doses of 20, 200, and 1,000 mg/kg/day, i.g., for 14 days
Mentions: Plumbagin and the PI extract had no significant influence on the profile of mouse body weight [Figure 3]. However, the treatments with plumbagin and the PI extract significantly elevated the levels of ALT and AST, compared with the control mice [Table 1]. While plumbagin significantly increased both plasma ALT and AST levels in a dose-dependent manner, the PI extract only increased the AST levels dose-dependently; the ALT levels were increased in a dose-independent manner.

Bottom Line: Plasma alanine transaminase (ALT) and aspartate transaminase (AST) levels, hepatic lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, and the ratio of reduced to oxidized glutathione (GSH/GSSG) were determined.Plumbagin and PI significantly increased plasma ALT and AST levels, hepatic lipid peroxidation, and GPx activity but significantly decreased hepatic SOD and CAT activities.The GSH/GSSG ratio was significantly reduced by plumbagin.

View Article: PubMed Central - PubMed

Affiliation: Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology, Khon Kaen University.

ABSTRACT

Background/aim: Plumbago indica (PI) L. and its active constituent, plumbagin, has been traditionally claimed for several pharmacological activities; however, there is little information regarding their toxicity. The present study aims to examine the effects of plumbagin and PI extract (PI) on hepatic histomorphology and antioxidative system in mice.

Materials and methods: Adult male intelligent character recognition mice were intragastrically administered plumbagin (1, 5, and 15 mg/kg/day) or PI (20, 200, and 1,000 mg/kg/day) consecutively for 14 days. Hepatic histomorphology was examined. Plasma alanine transaminase (ALT) and aspartate transaminase (AST) levels, hepatic lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities, and the ratio of reduced to oxidized glutathione (GSH/GSSG) were determined.

Results: Plumbagin and PI concentration-dependently induced hepatic injury based on histopathological changes via imbalance of antioxidative system. Plumbagin and PI significantly increased plasma ALT and AST levels, hepatic lipid peroxidation, and GPx activity but significantly decreased hepatic SOD and CAT activities. The GSH/GSSG ratio was significantly reduced by plumbagin.

Conclusion: Plumbagin and PI caused hepatotoxic effects in the mice by unbalancing of the redox defense system. Therefore, plumbagin and PI-containing supplements should be used cautiously, especially when consumed in high quantities or for long periods.

No MeSH data available.


Related in: MedlinePlus