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Cytogenetic relationships among Citrullus species in comparison with some genera of the tribe Benincaseae (Cucurbitaceae) as inferred from rDNA distribution patterns.

Li KP, Wu YX, Zhao H, Wang Y, Lü XM, Wang JM, Xu Y, Li ZY, Han YH - BMC Evol. Biol. (2016)

Bottom Line: The present study confirmed evolutionary closeness among cultivated watermelon (C. lanatus subsp. vulgaris), C. lanatus subsp. mucosospermus and C. colocynthis.Our result also supported that C. lanatus subsp. lanatus was not a wild form of the cultivated watermelon instead was a separate crop species.In addition, present cytogenetic analysis suggested that A. naudinianus was more closely related to Cucumis than to Citrullus or Acanthosicyos, but with a unique position and may be a link bridge between the Citrullus and the Cucumis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal University, Xuzhou, 221116, China.

ABSTRACT

Background: Comparative mapping of 5S and 45S rDNA by fluorescent in situ hybridization (FISH) technique is an excellent tool to determine cytogenetic relationships among closely related species.

Results: In this study, the number and position of 5S and 45S rDNA loci in all Citrullus species and subspecies were determined. The cultivated watermelon (C. lanatus subsp. vulgaris), C. lanatus subsp. mucosospermus, C. colocynthis and C. naudinianus (or Acanthosicyos naudinianus) had two 45S rDNA loci and one 5S rDNA locus which was located syntenic to one of the 45S rDNA loci. C. ecirrhosus and C. lanatus subsp. lanatus had one 45S rDNA locus and two 5S rDNA loci, each located on a different chromosome. C. rehmii had one 5S and one 45S rDNA locus positioned on different chromosomes. The distribution of 5S and 45S rDNA in several species belonging to other genera in Benincaseae tribe was also investigated. The distribution pattern of rDNAs showed a great difference among these species.

Conclusions: The present study confirmed evolutionary closeness among cultivated watermelon (C. lanatus subsp. vulgaris), C. lanatus subsp. mucosospermus and C. colocynthis. Our result also supported that C. lanatus subsp. lanatus was not a wild form of the cultivated watermelon instead was a separate crop species. In addition, present cytogenetic analysis suggested that A. naudinianus was more closely related to Cucumis than to Citrullus or Acanthosicyos, but with a unique position and may be a link bridge between the Citrullus and the Cucumis.

No MeSH data available.


FISH of 5S rDNA (red) and 45S rDNA (green) probes in eight species from other genera in Benincaseae tribe. aLagenaria siceraria. bZehneria mariothii. cMelothria pendula. dCucumis metuliferus. eBenincasa fistulosa. fCoccinia sessilifolia. gDiplocyclos palmatus. hCucumis sativus. Bars, 5 mm
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Fig2: FISH of 5S rDNA (red) and 45S rDNA (green) probes in eight species from other genera in Benincaseae tribe. aLagenaria siceraria. bZehneria mariothii. cMelothria pendula. dCucumis metuliferus. eBenincasa fistulosa. fCoccinia sessilifolia. gDiplocyclos palmatus. hCucumis sativus. Bars, 5 mm

Mentions: The distribution of 5S and 45S rDNA were also investigated in eight species from other genera in Benincaseae tribe, including Lagenaria siceraria (Fig. 2a), Zehneria mariothii (Fig. 2b), Melothria pendula (Fig. 2c), Cucumis metuliferus (Fig. 2d), Benincasa fistulosa (Fig. 2e), Coccinia sessilifolia (Fig. 2f), Diplocyclos palmatus (Fig. 2g) and Cucumis sativus (Fig. 2h) (Table 1). There was only one 5S locus (red) in these species except for B. fistulosa with two 5S loci (Fig. 2e). However, the number of 45S rDNA loci (green) showed a great difference. There were two 45S rDNA loci in L. siceraria (Fig. 2a), Z. mariothii (Fig. 2b), M. pendula (Fig. 2c), C. metuliferus (Fig. 2d) and B. fistulosa (Fig. 2e), three 45S rDNA loci in C. sessilifolia (Fig. 2f) and D. palmatus (Fig. 2g), and five 45S rDNA loci in C. sativas (Fig. 2h), respectively. The distribution pattern of 5S and 45S rDNA and chromosome number were exactly same between L. siceraria (Fig. 2a) and cultivated watermelon (Fig. 1a2, a4), also between C. sessilifolia (Fig. 2f) and D. palmatus (Fig. 2g).Fig. 2


Cytogenetic relationships among Citrullus species in comparison with some genera of the tribe Benincaseae (Cucurbitaceae) as inferred from rDNA distribution patterns.

Li KP, Wu YX, Zhao H, Wang Y, Lü XM, Wang JM, Xu Y, Li ZY, Han YH - BMC Evol. Biol. (2016)

FISH of 5S rDNA (red) and 45S rDNA (green) probes in eight species from other genera in Benincaseae tribe. aLagenaria siceraria. bZehneria mariothii. cMelothria pendula. dCucumis metuliferus. eBenincasa fistulosa. fCoccinia sessilifolia. gDiplocyclos palmatus. hCucumis sativus. Bars, 5 mm
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4835933&req=5

Fig2: FISH of 5S rDNA (red) and 45S rDNA (green) probes in eight species from other genera in Benincaseae tribe. aLagenaria siceraria. bZehneria mariothii. cMelothria pendula. dCucumis metuliferus. eBenincasa fistulosa. fCoccinia sessilifolia. gDiplocyclos palmatus. hCucumis sativus. Bars, 5 mm
Mentions: The distribution of 5S and 45S rDNA were also investigated in eight species from other genera in Benincaseae tribe, including Lagenaria siceraria (Fig. 2a), Zehneria mariothii (Fig. 2b), Melothria pendula (Fig. 2c), Cucumis metuliferus (Fig. 2d), Benincasa fistulosa (Fig. 2e), Coccinia sessilifolia (Fig. 2f), Diplocyclos palmatus (Fig. 2g) and Cucumis sativus (Fig. 2h) (Table 1). There was only one 5S locus (red) in these species except for B. fistulosa with two 5S loci (Fig. 2e). However, the number of 45S rDNA loci (green) showed a great difference. There were two 45S rDNA loci in L. siceraria (Fig. 2a), Z. mariothii (Fig. 2b), M. pendula (Fig. 2c), C. metuliferus (Fig. 2d) and B. fistulosa (Fig. 2e), three 45S rDNA loci in C. sessilifolia (Fig. 2f) and D. palmatus (Fig. 2g), and five 45S rDNA loci in C. sativas (Fig. 2h), respectively. The distribution pattern of 5S and 45S rDNA and chromosome number were exactly same between L. siceraria (Fig. 2a) and cultivated watermelon (Fig. 1a2, a4), also between C. sessilifolia (Fig. 2f) and D. palmatus (Fig. 2g).Fig. 2

Bottom Line: The present study confirmed evolutionary closeness among cultivated watermelon (C. lanatus subsp. vulgaris), C. lanatus subsp. mucosospermus and C. colocynthis.Our result also supported that C. lanatus subsp. lanatus was not a wild form of the cultivated watermelon instead was a separate crop species.In addition, present cytogenetic analysis suggested that A. naudinianus was more closely related to Cucumis than to Citrullus or Acanthosicyos, but with a unique position and may be a link bridge between the Citrullus and the Cucumis.

View Article: PubMed Central - PubMed

Affiliation: Institute of Integrative Plant Biology, School of Life Sciences, Jiangsu Normal University, Xuzhou, 221116, China.

ABSTRACT

Background: Comparative mapping of 5S and 45S rDNA by fluorescent in situ hybridization (FISH) technique is an excellent tool to determine cytogenetic relationships among closely related species.

Results: In this study, the number and position of 5S and 45S rDNA loci in all Citrullus species and subspecies were determined. The cultivated watermelon (C. lanatus subsp. vulgaris), C. lanatus subsp. mucosospermus, C. colocynthis and C. naudinianus (or Acanthosicyos naudinianus) had two 45S rDNA loci and one 5S rDNA locus which was located syntenic to one of the 45S rDNA loci. C. ecirrhosus and C. lanatus subsp. lanatus had one 45S rDNA locus and two 5S rDNA loci, each located on a different chromosome. C. rehmii had one 5S and one 45S rDNA locus positioned on different chromosomes. The distribution of 5S and 45S rDNA in several species belonging to other genera in Benincaseae tribe was also investigated. The distribution pattern of rDNAs showed a great difference among these species.

Conclusions: The present study confirmed evolutionary closeness among cultivated watermelon (C. lanatus subsp. vulgaris), C. lanatus subsp. mucosospermus and C. colocynthis. Our result also supported that C. lanatus subsp. lanatus was not a wild form of the cultivated watermelon instead was a separate crop species. In addition, present cytogenetic analysis suggested that A. naudinianus was more closely related to Cucumis than to Citrullus or Acanthosicyos, but with a unique position and may be a link bridge between the Citrullus and the Cucumis.

No MeSH data available.