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Increased CD56(bright) NK cells in HIV-HCV co-infection and HCV mono-infection are associated with distinctive alterations of their phenotype.

Bhardwaj S, Ahmad F, Wedemeyer H, Cornberg M, Schulze Zur Wiesch J, van Lunzen J, Sarin SK, Schmidt RE, Meyer-Olson D - Virol. J. (2016)

Bottom Line: HIV-HCV co-infection is associated with accelerated progression to hepatic fibrosis, cirrhosis and hepatocellular carcinoma than HCV mono-infection.The expression of NKp46 in HIV-HCV co-infected group was significantly upregulated as compared to both HIV as well as HCV mono-infections while NKp30 expression in the HIV-HCV co-infected group significantly differed as compared to HIV mono-infection.HCV mono-infection has a dominant effect on NKp30 expression while NKG2D and CD127 expressions remained same in all the groups.

View Article: PubMed Central - PubMed

Affiliation: Institute of Liver and Billiary Sciences, New Delhi, India.

ABSTRACT

Background: HIV-HCV co-infection is associated with accelerated progression to hepatic fibrosis, cirrhosis and hepatocellular carcinoma than HCV mono-infection. The contribution of innate immunity during HIV-HCV co-infection has been a relatively under-investigated area. Natural killer (NK) cells are pivotal sentinels of innate immunity against viruses and tumour cells. In this study we evaluated the effect of HIV-HCV co-infection on peripheral blood NK cell subsets with emphasis on the phenotype of CD56(bright) NK cells.

Methods: Sixty patients were included in the study; HIV mono-infected (n = 12), HCV mono-infected (n = 15), HCV-HIV co-infected (n = 21) and healthy controls (n = 16). PBMCs were isolated and immunophenotyping of NK cells was performed by flowcytometry.

Results: We observed an expansion of CD56(bright) NK cell subset in HIV-HCV co-infection as compared to healthy controls and HIV mono-infected group. All the infected groups had an upregulated expression of the activating receptor NKG2D on CD56(bright) NK cells in comparison to healthy controls while not differing amongst themselves. The expression of NKp46 in HIV-HCV co-infected group was significantly upregulated as compared to both HIV as well as HCV mono-infections while NKp30 expression in the HIV-HCV co-infected group significantly differed as compared to HIV mono-infection. The CD56(bright) NK cell subset was activated in HIV-HCV co-infection as assessed by the expression of CD69 as compared to healthy controls but was significantly downregulated in comparison to HIV mono-infection. CD95 expression on CD56(bright) NK cells followed the same pattern where there was an increased expression of CD95 in HIV mono-infection and HIV-HCV co-infection as compared to healthy controls. In contrast to CD69 expression, CD95 expression in HCV mono-infection was decreased when compared to HIV mono-infection and HIV-HCV co-infection. Finally, expression of CXCR3 on CD56(bright) NK cells was increased in HIV-HCV co-infection in comparison to HIV mono-infection while remaining similar to HCV mono-infection.

Conclusion: Thus, HIV-HCV co-infection is able to modulate the phenotype of CD56(bright) NK cell subset in a unique way such that NKp46 and CXCR3 expressions are distinct for co-infection while both mono-infections have an additive effect on CD56(bright), CD69 with CD95 expressions. HCV mono-infection has a dominant effect on NKp30 expression while NKG2D and CD127 expressions remained same in all the groups.

No MeSH data available.


Related in: MedlinePlus

Upregulated expression of NKG2D and NCRs on CD56brightCD16+/- NK cells in HIV-HCV co-infection. a Representative examples of flow cytometric analysis of C-type lectin receptor NKG2D and NCRs NKp46 and NKp30 expression on CD56brightCD16+/- NK cells. b Surface expression of NKG2D on CD56brightCD16+/- NK cell subset in healthy controls (n = 15), HIV mono-infected (n =12), HIV-HCV co-infected (n = 12) and HCV mono-infected patients (n = 15). c Surface expression of NKp46 on CD56brightCD16+/- NK cell subset in healthy controls (n = 13), HIV mono-infected (n =11), HIV-HCV co-infected (n = 13) and HCV mono-infected patients (n = 15). d Surface expression of NKp30 on CD56brightCD16+/- NK cell subset in healthy controls (n = 16), HIV mono-infected (n =11), HIV-HCV co-infected (n = 12) and HCV mono-infected patients (n = 12). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns - not significant (P > 0.05). In the dot plot figures horizontal line represents Log % Mean ± SEM
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Fig2: Upregulated expression of NKG2D and NCRs on CD56brightCD16+/- NK cells in HIV-HCV co-infection. a Representative examples of flow cytometric analysis of C-type lectin receptor NKG2D and NCRs NKp46 and NKp30 expression on CD56brightCD16+/- NK cells. b Surface expression of NKG2D on CD56brightCD16+/- NK cell subset in healthy controls (n = 15), HIV mono-infected (n =12), HIV-HCV co-infected (n = 12) and HCV mono-infected patients (n = 15). c Surface expression of NKp46 on CD56brightCD16+/- NK cell subset in healthy controls (n = 13), HIV mono-infected (n =11), HIV-HCV co-infected (n = 13) and HCV mono-infected patients (n = 15). d Surface expression of NKp30 on CD56brightCD16+/- NK cell subset in healthy controls (n = 16), HIV mono-infected (n =11), HIV-HCV co-infected (n = 12) and HCV mono-infected patients (n = 12). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns - not significant (P > 0.05). In the dot plot figures horizontal line represents Log % Mean ± SEM

Mentions: The representative flow cytometry plots of NKG2D and NCRs, NKp46 and NKp30 expression on CD56bright NK cells is shown in Fig. 2a. All the infected groups did not differ significantly for NKG2D expression on the CD56bright NK cell subset; however its expression was significantly up-regulated on all the infected groups as compared to healthy controls (Fig. 2b).Fig. 2


Increased CD56(bright) NK cells in HIV-HCV co-infection and HCV mono-infection are associated with distinctive alterations of their phenotype.

Bhardwaj S, Ahmad F, Wedemeyer H, Cornberg M, Schulze Zur Wiesch J, van Lunzen J, Sarin SK, Schmidt RE, Meyer-Olson D - Virol. J. (2016)

Upregulated expression of NKG2D and NCRs on CD56brightCD16+/- NK cells in HIV-HCV co-infection. a Representative examples of flow cytometric analysis of C-type lectin receptor NKG2D and NCRs NKp46 and NKp30 expression on CD56brightCD16+/- NK cells. b Surface expression of NKG2D on CD56brightCD16+/- NK cell subset in healthy controls (n = 15), HIV mono-infected (n =12), HIV-HCV co-infected (n = 12) and HCV mono-infected patients (n = 15). c Surface expression of NKp46 on CD56brightCD16+/- NK cell subset in healthy controls (n = 13), HIV mono-infected (n =11), HIV-HCV co-infected (n = 13) and HCV mono-infected patients (n = 15). d Surface expression of NKp30 on CD56brightCD16+/- NK cell subset in healthy controls (n = 16), HIV mono-infected (n =11), HIV-HCV co-infected (n = 12) and HCV mono-infected patients (n = 12). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns - not significant (P > 0.05). In the dot plot figures horizontal line represents Log % Mean ± SEM
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4835889&req=5

Fig2: Upregulated expression of NKG2D and NCRs on CD56brightCD16+/- NK cells in HIV-HCV co-infection. a Representative examples of flow cytometric analysis of C-type lectin receptor NKG2D and NCRs NKp46 and NKp30 expression on CD56brightCD16+/- NK cells. b Surface expression of NKG2D on CD56brightCD16+/- NK cell subset in healthy controls (n = 15), HIV mono-infected (n =12), HIV-HCV co-infected (n = 12) and HCV mono-infected patients (n = 15). c Surface expression of NKp46 on CD56brightCD16+/- NK cell subset in healthy controls (n = 13), HIV mono-infected (n =11), HIV-HCV co-infected (n = 13) and HCV mono-infected patients (n = 15). d Surface expression of NKp30 on CD56brightCD16+/- NK cell subset in healthy controls (n = 16), HIV mono-infected (n =11), HIV-HCV co-infected (n = 12) and HCV mono-infected patients (n = 12). *, P < 0.05; **, P < 0.01; ***, P < 0.001; ns - not significant (P > 0.05). In the dot plot figures horizontal line represents Log % Mean ± SEM
Mentions: The representative flow cytometry plots of NKG2D and NCRs, NKp46 and NKp30 expression on CD56bright NK cells is shown in Fig. 2a. All the infected groups did not differ significantly for NKG2D expression on the CD56bright NK cell subset; however its expression was significantly up-regulated on all the infected groups as compared to healthy controls (Fig. 2b).Fig. 2

Bottom Line: HIV-HCV co-infection is associated with accelerated progression to hepatic fibrosis, cirrhosis and hepatocellular carcinoma than HCV mono-infection.The expression of NKp46 in HIV-HCV co-infected group was significantly upregulated as compared to both HIV as well as HCV mono-infections while NKp30 expression in the HIV-HCV co-infected group significantly differed as compared to HIV mono-infection.HCV mono-infection has a dominant effect on NKp30 expression while NKG2D and CD127 expressions remained same in all the groups.

View Article: PubMed Central - PubMed

Affiliation: Institute of Liver and Billiary Sciences, New Delhi, India.

ABSTRACT

Background: HIV-HCV co-infection is associated with accelerated progression to hepatic fibrosis, cirrhosis and hepatocellular carcinoma than HCV mono-infection. The contribution of innate immunity during HIV-HCV co-infection has been a relatively under-investigated area. Natural killer (NK) cells are pivotal sentinels of innate immunity against viruses and tumour cells. In this study we evaluated the effect of HIV-HCV co-infection on peripheral blood NK cell subsets with emphasis on the phenotype of CD56(bright) NK cells.

Methods: Sixty patients were included in the study; HIV mono-infected (n = 12), HCV mono-infected (n = 15), HCV-HIV co-infected (n = 21) and healthy controls (n = 16). PBMCs were isolated and immunophenotyping of NK cells was performed by flowcytometry.

Results: We observed an expansion of CD56(bright) NK cell subset in HIV-HCV co-infection as compared to healthy controls and HIV mono-infected group. All the infected groups had an upregulated expression of the activating receptor NKG2D on CD56(bright) NK cells in comparison to healthy controls while not differing amongst themselves. The expression of NKp46 in HIV-HCV co-infected group was significantly upregulated as compared to both HIV as well as HCV mono-infections while NKp30 expression in the HIV-HCV co-infected group significantly differed as compared to HIV mono-infection. The CD56(bright) NK cell subset was activated in HIV-HCV co-infection as assessed by the expression of CD69 as compared to healthy controls but was significantly downregulated in comparison to HIV mono-infection. CD95 expression on CD56(bright) NK cells followed the same pattern where there was an increased expression of CD95 in HIV mono-infection and HIV-HCV co-infection as compared to healthy controls. In contrast to CD69 expression, CD95 expression in HCV mono-infection was decreased when compared to HIV mono-infection and HIV-HCV co-infection. Finally, expression of CXCR3 on CD56(bright) NK cells was increased in HIV-HCV co-infection in comparison to HIV mono-infection while remaining similar to HCV mono-infection.

Conclusion: Thus, HIV-HCV co-infection is able to modulate the phenotype of CD56(bright) NK cell subset in a unique way such that NKp46 and CXCR3 expressions are distinct for co-infection while both mono-infections have an additive effect on CD56(bright), CD69 with CD95 expressions. HCV mono-infection has a dominant effect on NKp30 expression while NKG2D and CD127 expressions remained same in all the groups.

No MeSH data available.


Related in: MedlinePlus