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Blood meal sources of wild and domestic Triatoma infestans (Hemiptera: Reduviidae) in Bolivia: connectivity between cycles of transmission of Trypanosoma cruzi.

Buitrago R, Bosseno MF, Depickère S, Waleckx E, Salas R, Aliaga C, Barnabé C, Brenière SF - Parasit Vectors (2016)

Bottom Line: Interestingly, blood from wild animals was identified in triatomines captured in the peridomestic and domestic environment, and blood from domestic animals was found in triatomines captured in the wild, revealing links between wild and domestic cycles of T. cruzi transmission.The current study suggests that wild T. infestans attack humans in the wild, but is also able to bite humans in domestic settings before going back to its natural environment.These results support the risk to human health posed by wild populations of T. infestans.

View Article: PubMed Central - PubMed

Affiliation: IRD, Institut de Recherche pour le Développement, UMR INTERTRYP, (IRD-CIRAD), Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatidés, 911 Av. Agropolis, Montpellier, cédex 5, 34394, France. rosiob8@gmail.com.

ABSTRACT

Background: Chagas disease is a major public health problem in Latin America. Its etiologic agent, Trypanosoma cruzi, is mainly transmitted through the contaminated faeces of blood-sucking insects called triatomines. Triatoma infestans is the main vector in various countries in South America and recently, several foci of wild populations of this species have been described in Bolivia and other countries. These wild populations are suspected of affecting the success of insecticide control campaigns being carried out in South America. To assess the risk that these T. infestans populations pose to human health, it is helpful to determine blood meal sources.

Methods: In the present work, blood meals were identified in various Bolivian wild T. infestans populations and in three specific areas, in both wild and intra-peridomestic populations to assess the links between wild and domestic cycles of T. cruzi transmission. PCR-HDA and sequencing of Cytb gene were used to identify these blood meal sources.

Results and discussion: Fourteen vertebrate species were identified as wild blood meal sources. Of those, the most prevalent species were two Andean endemic rodents, Octodontomys gliroides (36%) and Galea musteloides (30%), while humans were the third most prevalent source (18.7%). Of 163 blood meals from peridomestic areas, more than half were chickens, and the others were generally domestic animals or humans. Interestingly, blood from wild animals was identified in triatomines captured in the peridomestic and domestic environment, and blood from domestic animals was found in triatomines captured in the wild, revealing links between wild and domestic cycles of T. cruzi transmission.

Conclusion: The current study suggests that wild T. infestans attack humans in the wild, but is also able to bite humans in domestic settings before going back to its natural environment. These results support the risk to human health posed by wild populations of T. infestans.

No MeSH data available.


Related in: MedlinePlus

Vertebrate blood meal sources of intra-peridomestic T. infestans identified by PCR-HDA and sequencing in three specific areas (Quillacollo, Thago Thago and Sapini). The ellipses show the wild animals identified
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Fig3: Vertebrate blood meal sources of intra-peridomestic T. infestans identified by PCR-HDA and sequencing in three specific areas (Quillacollo, Thago Thago and Sapini). The ellipses show the wild animals identified

Mentions: In the sylvatic area around Sapini, 50 intestinal contents were processed and 17 blood meal sources (34 %) were identified. Three species of wild rodents and human were identified (Table 3). The most prevalent blood meal source identified in both adult specimens and nymphal instars was O. gliroides (58.8 %). The second most prevalent species was H. sapiens (23.5 %) found in three adult specimens and a 5th nymphal instar. In peridomiciles, a total of 13 samples were processed and the blood meal sources of seven samples were identified (53.8 %). The most prevalent blood sources were chicken (71 %) and human (Table 3, Fig. 3).Table 3


Blood meal sources of wild and domestic Triatoma infestans (Hemiptera: Reduviidae) in Bolivia: connectivity between cycles of transmission of Trypanosoma cruzi.

Buitrago R, Bosseno MF, Depickère S, Waleckx E, Salas R, Aliaga C, Barnabé C, Brenière SF - Parasit Vectors (2016)

Vertebrate blood meal sources of intra-peridomestic T. infestans identified by PCR-HDA and sequencing in three specific areas (Quillacollo, Thago Thago and Sapini). The ellipses show the wild animals identified
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4835887&req=5

Fig3: Vertebrate blood meal sources of intra-peridomestic T. infestans identified by PCR-HDA and sequencing in three specific areas (Quillacollo, Thago Thago and Sapini). The ellipses show the wild animals identified
Mentions: In the sylvatic area around Sapini, 50 intestinal contents were processed and 17 blood meal sources (34 %) were identified. Three species of wild rodents and human were identified (Table 3). The most prevalent blood meal source identified in both adult specimens and nymphal instars was O. gliroides (58.8 %). The second most prevalent species was H. sapiens (23.5 %) found in three adult specimens and a 5th nymphal instar. In peridomiciles, a total of 13 samples were processed and the blood meal sources of seven samples were identified (53.8 %). The most prevalent blood sources were chicken (71 %) and human (Table 3, Fig. 3).Table 3

Bottom Line: Interestingly, blood from wild animals was identified in triatomines captured in the peridomestic and domestic environment, and blood from domestic animals was found in triatomines captured in the wild, revealing links between wild and domestic cycles of T. cruzi transmission.The current study suggests that wild T. infestans attack humans in the wild, but is also able to bite humans in domestic settings before going back to its natural environment.These results support the risk to human health posed by wild populations of T. infestans.

View Article: PubMed Central - PubMed

Affiliation: IRD, Institut de Recherche pour le Développement, UMR INTERTRYP, (IRD-CIRAD), Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatidés, 911 Av. Agropolis, Montpellier, cédex 5, 34394, France. rosiob8@gmail.com.

ABSTRACT

Background: Chagas disease is a major public health problem in Latin America. Its etiologic agent, Trypanosoma cruzi, is mainly transmitted through the contaminated faeces of blood-sucking insects called triatomines. Triatoma infestans is the main vector in various countries in South America and recently, several foci of wild populations of this species have been described in Bolivia and other countries. These wild populations are suspected of affecting the success of insecticide control campaigns being carried out in South America. To assess the risk that these T. infestans populations pose to human health, it is helpful to determine blood meal sources.

Methods: In the present work, blood meals were identified in various Bolivian wild T. infestans populations and in three specific areas, in both wild and intra-peridomestic populations to assess the links between wild and domestic cycles of T. cruzi transmission. PCR-HDA and sequencing of Cytb gene were used to identify these blood meal sources.

Results and discussion: Fourteen vertebrate species were identified as wild blood meal sources. Of those, the most prevalent species were two Andean endemic rodents, Octodontomys gliroides (36%) and Galea musteloides (30%), while humans were the third most prevalent source (18.7%). Of 163 blood meals from peridomestic areas, more than half were chickens, and the others were generally domestic animals or humans. Interestingly, blood from wild animals was identified in triatomines captured in the peridomestic and domestic environment, and blood from domestic animals was found in triatomines captured in the wild, revealing links between wild and domestic cycles of T. cruzi transmission.

Conclusion: The current study suggests that wild T. infestans attack humans in the wild, but is also able to bite humans in domestic settings before going back to its natural environment. These results support the risk to human health posed by wild populations of T. infestans.

No MeSH data available.


Related in: MedlinePlus