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Vitamin D3 stimulates embryonic stem cells but inhibits migration and growth of ovarian cancer and teratocarcinoma cell lines.

Abdelbaset-Ismail A, Pedziwiatr D, Suszyńska E, Sluczanowska-Glabowska S, Schneider G, Kakar SS, Ratajczak MZ - J Ovarian Res (2016)

Bottom Line: We evaluated expression of Vitamin D3 receptor (VDR) in these cells as well as effect of vitamin D3 exposure on cell proliferation and migration.We also found that the ESD3 murine immortalized embryonic stem cell line and normal, pluripotent, germline-marker-positive very small embryonic-like stem cells (VSELs) isolated from adult tissues are stimulated by vitamin D3, which suggests that vitamin D3 affects the earliest stages of embryogenesis.We found that however all normal and malignant germ-line derived cells express functional VDR, Vitamin D3 differently affects their proliferation and migration.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S. Floyd Street, Rm. 107, Louisville, KY, 40202, USA.

ABSTRACT

Background: Deficiency in Vitamin D3 (cholecalciferol) may predispose to some malignancies, including gonadal tumors and in experimental models vitamin D3 has been proven to inhibit the growth of cancer cells. To learn more about the potential role of vitamin D3 in cancerogenesis, we evaluated the expression and functionality of the vitamin D receptor (VDR) and its role in metastasis of ovarian cancer cells and of murine and human teratocarcinoma cell lines.

Methods: In our studies we employed murine embrynic stem cells (ESD3), murine (P19) and human (NTERA-2) teratocarcimona cells lines, human ovarian cancer cells (A2780) as well as purified murine and human purified very small embryonic like stem cells (VSELs). We evaluated expression of Vitamin D3 receptor (VDR) in these cells as well as effect of vitamin D3 exposure on cell proliferation and migration.

Results: We here provide also more evidence for the role of vitamin D3 in germline-derived malignancies, and this evidence supports the proposal that vitamin D3 treatment inhibits growth and metastatic potential of several germline-derived malignancies. We also found that the ESD3 murine immortalized embryonic stem cell line and normal, pluripotent, germline-marker-positive very small embryonic-like stem cells (VSELs) isolated from adult tissues are stimulated by vitamin D3, which suggests that vitamin D3 affects the earliest stages of embryogenesis.

Conclusions: We found that however all normal and malignant germ-line derived cells express functional VDR, Vitamin D3 differently affects their proliferation and migration. We postulate that while Vitamin D3 as anticancer drug inhibits proliferation of malignant cells, it may protect normal stem cells that play an important role in development and tissue/organ regeneration.

No MeSH data available.


Related in: MedlinePlus

1,25-dihydroxyvitamin D3 suppresses proliferation of human and murine germline-derived cell lines. Proliferation of the A2780 human ovarian cancer cell line (Panel a) as well as the NTERA-2 human teratocarcinoma (Panel b) and mP19 murine embryonal teratocarcinoma (Panel c) cell lines was significantly decreased by 1,25-dihydroxyvitamin D3 in a dose-dependent manner in comparison with cells treated with vehicle only. All proliferation experiments were done either in RPMI (for A2780), DMEM (for NTERA-2), or αMEM (for P19) culture medium containing 0.5 % BSA for 72 h using 0.3 × 104 cells/well in a 96-well plate. The negative control values are normalized to 100 %. For each cell line, the experiment was repeated twice in triplicate with similar results
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Fig4: 1,25-dihydroxyvitamin D3 suppresses proliferation of human and murine germline-derived cell lines. Proliferation of the A2780 human ovarian cancer cell line (Panel a) as well as the NTERA-2 human teratocarcinoma (Panel b) and mP19 murine embryonal teratocarcinoma (Panel c) cell lines was significantly decreased by 1,25-dihydroxyvitamin D3 in a dose-dependent manner in comparison with cells treated with vehicle only. All proliferation experiments were done either in RPMI (for A2780), DMEM (for NTERA-2), or αMEM (for P19) culture medium containing 0.5 % BSA for 72 h using 0.3 × 104 cells/well in a 96-well plate. The negative control values are normalized to 100 %. For each cell line, the experiment was repeated twice in triplicate with similar results

Mentions: In parallel, these apoptotic changes corresponded with an inhibitory effect of vitamin D3 on proliferation of human and murine germline-derived malignant cell lines (Fig. 4).Fig. 4


Vitamin D3 stimulates embryonic stem cells but inhibits migration and growth of ovarian cancer and teratocarcinoma cell lines.

Abdelbaset-Ismail A, Pedziwiatr D, Suszyńska E, Sluczanowska-Glabowska S, Schneider G, Kakar SS, Ratajczak MZ - J Ovarian Res (2016)

1,25-dihydroxyvitamin D3 suppresses proliferation of human and murine germline-derived cell lines. Proliferation of the A2780 human ovarian cancer cell line (Panel a) as well as the NTERA-2 human teratocarcinoma (Panel b) and mP19 murine embryonal teratocarcinoma (Panel c) cell lines was significantly decreased by 1,25-dihydroxyvitamin D3 in a dose-dependent manner in comparison with cells treated with vehicle only. All proliferation experiments were done either in RPMI (for A2780), DMEM (for NTERA-2), or αMEM (for P19) culture medium containing 0.5 % BSA for 72 h using 0.3 × 104 cells/well in a 96-well plate. The negative control values are normalized to 100 %. For each cell line, the experiment was repeated twice in triplicate with similar results
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4835879&req=5

Fig4: 1,25-dihydroxyvitamin D3 suppresses proliferation of human and murine germline-derived cell lines. Proliferation of the A2780 human ovarian cancer cell line (Panel a) as well as the NTERA-2 human teratocarcinoma (Panel b) and mP19 murine embryonal teratocarcinoma (Panel c) cell lines was significantly decreased by 1,25-dihydroxyvitamin D3 in a dose-dependent manner in comparison with cells treated with vehicle only. All proliferation experiments were done either in RPMI (for A2780), DMEM (for NTERA-2), or αMEM (for P19) culture medium containing 0.5 % BSA for 72 h using 0.3 × 104 cells/well in a 96-well plate. The negative control values are normalized to 100 %. For each cell line, the experiment was repeated twice in triplicate with similar results
Mentions: In parallel, these apoptotic changes corresponded with an inhibitory effect of vitamin D3 on proliferation of human and murine germline-derived malignant cell lines (Fig. 4).Fig. 4

Bottom Line: We evaluated expression of Vitamin D3 receptor (VDR) in these cells as well as effect of vitamin D3 exposure on cell proliferation and migration.We also found that the ESD3 murine immortalized embryonic stem cell line and normal, pluripotent, germline-marker-positive very small embryonic-like stem cells (VSELs) isolated from adult tissues are stimulated by vitamin D3, which suggests that vitamin D3 affects the earliest stages of embryogenesis.We found that however all normal and malignant germ-line derived cells express functional VDR, Vitamin D3 differently affects their proliferation and migration.

View Article: PubMed Central - PubMed

Affiliation: Stem Cell Institute at James Graham Brown Cancer Center, University of Louisville, 500 S. Floyd Street, Rm. 107, Louisville, KY, 40202, USA.

ABSTRACT

Background: Deficiency in Vitamin D3 (cholecalciferol) may predispose to some malignancies, including gonadal tumors and in experimental models vitamin D3 has been proven to inhibit the growth of cancer cells. To learn more about the potential role of vitamin D3 in cancerogenesis, we evaluated the expression and functionality of the vitamin D receptor (VDR) and its role in metastasis of ovarian cancer cells and of murine and human teratocarcinoma cell lines.

Methods: In our studies we employed murine embrynic stem cells (ESD3), murine (P19) and human (NTERA-2) teratocarcimona cells lines, human ovarian cancer cells (A2780) as well as purified murine and human purified very small embryonic like stem cells (VSELs). We evaluated expression of Vitamin D3 receptor (VDR) in these cells as well as effect of vitamin D3 exposure on cell proliferation and migration.

Results: We here provide also more evidence for the role of vitamin D3 in germline-derived malignancies, and this evidence supports the proposal that vitamin D3 treatment inhibits growth and metastatic potential of several germline-derived malignancies. We also found that the ESD3 murine immortalized embryonic stem cell line and normal, pluripotent, germline-marker-positive very small embryonic-like stem cells (VSELs) isolated from adult tissues are stimulated by vitamin D3, which suggests that vitamin D3 affects the earliest stages of embryogenesis.

Conclusions: We found that however all normal and malignant germ-line derived cells express functional VDR, Vitamin D3 differently affects their proliferation and migration. We postulate that while Vitamin D3 as anticancer drug inhibits proliferation of malignant cells, it may protect normal stem cells that play an important role in development and tissue/organ regeneration.

No MeSH data available.


Related in: MedlinePlus