Limits...
Effect of the PGD2-DP signaling pathway on primary cultured rat hippocampal neuron injury caused by aluminum overload.

Ma J, Yang Q, Wei Y, Yang Y, Ji C, Hu X, Mai S, Kuang S, Tian X, Luo Y, Liang G, Yang J - Sci Rep (2016)

Bottom Line: BW245C reduced the Ca(2+) fluorescence intensity and protected the neurons.DK-PGD2 increased the intensity of Ca(2+) fluorescence, while CAY10471 had the opposite effect.In conclusion, contrary to the effect of DP2, the PGD2-DP1 signaling pathway protects against the primary cultured rat hippocampal neuronal injury caused by aluminum overload.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Chongqing Medical University, the Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing 400016, China.

ABSTRACT
In the present study, the agonists and antagonists of DP receptor were used to examine whether the PGD2-DP signaling pathway affects neuronal function. Primary cultured hippocampal neuron was prepared and treated with aluminum maltolate (100 μM) to establish the neuronal damage model. PGD2 and cAMP content was detected by ELISA. L-PGDS and DPs mRNA and protein expression were measured by RT-PCR and Western blotting, respectively. The aluminium-load neuron was treated with the DP1 agonist BW245C, the DP1 antagonist BWA868C, the DP2 agonist DK-PGD2, and the DP2 antagonist CAY10471, respectively. Neuronal pathomorphology was observed using H-E staining. The cell viability and the lactate dehydrogenase leakage rates of neurons were measured with MTT and LDH kit, respectively. Ca(2+) level was detected by Fluo-3/AM. In the model group, the MTT values obviously decreased; LDH leakage rates and PGD2 content increased significantly; L-PGDS, DP1 mRNA and protein expressions increased, and DP2 level decreased. BW245C reduced the Ca(2+) fluorescence intensity and protected the neurons. DK-PGD2 increased the intensity of Ca(2+) fluorescence, while CAY10471 had the opposite effect. In conclusion, contrary to the effect of DP2, the PGD2-DP1 signaling pathway protects against the primary cultured rat hippocampal neuronal injury caused by aluminum overload.

No MeSH data available.


Related in: MedlinePlus

Dose-dependent effects of Al (malt)3 and maltol on neuronal viability detected by the method of MTT.The 100, 200 and 400 M of Al (malt)3 decreased the neuron viability significantly whereas the neuron viability at 50 μM decreased slightly. The concentration of Al3+ at 100 μM was suitable for the damage model. There was no considerable difference between the control group and the solvent control (maltol) (100–1200 μM) group. Values were mean ± SD of six individual experiments (n = 6, **P < 0.01 vs. control group, one-way ANOVA with Dunnett’s multiple comparisons).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4835855&req=5

f2: Dose-dependent effects of Al (malt)3 and maltol on neuronal viability detected by the method of MTT.The 100, 200 and 400 M of Al (malt)3 decreased the neuron viability significantly whereas the neuron viability at 50 μM decreased slightly. The concentration of Al3+ at 100 μM was suitable for the damage model. There was no considerable difference between the control group and the solvent control (maltol) (100–1200 μM) group. Values were mean ± SD of six individual experiments (n = 6, **P < 0.01 vs. control group, one-way ANOVA with Dunnett’s multiple comparisons).

Mentions: The results showed that compared with the control group, the neuron viability decreased significantly in the Al3+-treated group at the concentration of 100, 200 and 400 μM with the neuron survival rates of 69.17%, 39.97% and 27.66%, respectively. However, the neuron survival rate was 95.47% at the concentration of 50 μM of Al (malt)3, which was no substantial difference compared with the control group. There was no considerable difference between the control group and the solvent control (maltol) (100–1200 μM) group. Considering each ion of Al is bound to three molecules of maltolate, 100 μM of Al (malt)3 and 300 μM of maltol were used in following experiments to evaluate the effects of PGD2-DP signaling on Al-load neuron (Fig. 2).


Effect of the PGD2-DP signaling pathway on primary cultured rat hippocampal neuron injury caused by aluminum overload.

Ma J, Yang Q, Wei Y, Yang Y, Ji C, Hu X, Mai S, Kuang S, Tian X, Luo Y, Liang G, Yang J - Sci Rep (2016)

Dose-dependent effects of Al (malt)3 and maltol on neuronal viability detected by the method of MTT.The 100, 200 and 400 M of Al (malt)3 decreased the neuron viability significantly whereas the neuron viability at 50 μM decreased slightly. The concentration of Al3+ at 100 μM was suitable for the damage model. There was no considerable difference between the control group and the solvent control (maltol) (100–1200 μM) group. Values were mean ± SD of six individual experiments (n = 6, **P < 0.01 vs. control group, one-way ANOVA with Dunnett’s multiple comparisons).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4835855&req=5

f2: Dose-dependent effects of Al (malt)3 and maltol on neuronal viability detected by the method of MTT.The 100, 200 and 400 M of Al (malt)3 decreased the neuron viability significantly whereas the neuron viability at 50 μM decreased slightly. The concentration of Al3+ at 100 μM was suitable for the damage model. There was no considerable difference between the control group and the solvent control (maltol) (100–1200 μM) group. Values were mean ± SD of six individual experiments (n = 6, **P < 0.01 vs. control group, one-way ANOVA with Dunnett’s multiple comparisons).
Mentions: The results showed that compared with the control group, the neuron viability decreased significantly in the Al3+-treated group at the concentration of 100, 200 and 400 μM with the neuron survival rates of 69.17%, 39.97% and 27.66%, respectively. However, the neuron survival rate was 95.47% at the concentration of 50 μM of Al (malt)3, which was no substantial difference compared with the control group. There was no considerable difference between the control group and the solvent control (maltol) (100–1200 μM) group. Considering each ion of Al is bound to three molecules of maltolate, 100 μM of Al (malt)3 and 300 μM of maltol were used in following experiments to evaluate the effects of PGD2-DP signaling on Al-load neuron (Fig. 2).

Bottom Line: BW245C reduced the Ca(2+) fluorescence intensity and protected the neurons.DK-PGD2 increased the intensity of Ca(2+) fluorescence, while CAY10471 had the opposite effect.In conclusion, contrary to the effect of DP2, the PGD2-DP1 signaling pathway protects against the primary cultured rat hippocampal neuronal injury caused by aluminum overload.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Chongqing Medical University, the Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing 400016, China.

ABSTRACT
In the present study, the agonists and antagonists of DP receptor were used to examine whether the PGD2-DP signaling pathway affects neuronal function. Primary cultured hippocampal neuron was prepared and treated with aluminum maltolate (100 μM) to establish the neuronal damage model. PGD2 and cAMP content was detected by ELISA. L-PGDS and DPs mRNA and protein expression were measured by RT-PCR and Western blotting, respectively. The aluminium-load neuron was treated with the DP1 agonist BW245C, the DP1 antagonist BWA868C, the DP2 agonist DK-PGD2, and the DP2 antagonist CAY10471, respectively. Neuronal pathomorphology was observed using H-E staining. The cell viability and the lactate dehydrogenase leakage rates of neurons were measured with MTT and LDH kit, respectively. Ca(2+) level was detected by Fluo-3/AM. In the model group, the MTT values obviously decreased; LDH leakage rates and PGD2 content increased significantly; L-PGDS, DP1 mRNA and protein expressions increased, and DP2 level decreased. BW245C reduced the Ca(2+) fluorescence intensity and protected the neurons. DK-PGD2 increased the intensity of Ca(2+) fluorescence, while CAY10471 had the opposite effect. In conclusion, contrary to the effect of DP2, the PGD2-DP1 signaling pathway protects against the primary cultured rat hippocampal neuronal injury caused by aluminum overload.

No MeSH data available.


Related in: MedlinePlus