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The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens.

Psifidi A, Fife M, Howell J, Matika O, van Diemen PM, Kuo R, Smith J, Hocking PM, Salmon N, Jones MA, Hume DA, Banos G, Stevens MP, Kaiser P - BMC Genomics (2016)

Bottom Line: The level of colonisation with Campylobacter jejuni following experimental infection was found to be a quantitative trait.Finally, gene expression analyses were performed for some of the candidate resistance genes to support the results.Two of the QTLs for Campylobacter resistance are co-located with Salmonella resistance loci, indicating that it may be possible to breed simultaneously for enhanced resistance to both zoonoses.

View Article: PubMed Central - PubMed

Affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK. Androniki.psifidi@roslin.ed.ac.uk.

ABSTRACT

Background: Campylobacter is the leading cause of foodborne diarrhoeal illness in humans and is mostly acquired from consumption or handling of contaminated poultry meat. In the absence of effective licensed vaccines and inhibitors, selection for chickens with increased resistance to Campylobacter could potentially reduce its subsequent entry into the food chain. Campylobacter intestinal colonisation levels are influenced by the host genetics of the chicken. In the present study, two chicken populations were used to investigate the genetic architecture of avian resistance to colonisation: (i) a back-cross of two White Leghorn derived inbred lines [(61 x N) x N] known to differ in resistance to Campylobacter colonisation and (ii) a 9(th) generation advanced intercross (61 x N) line.

Results: The level of colonisation with Campylobacter jejuni following experimental infection was found to be a quantitative trait. A back-cross experiment using 1,243 fully informative single nucleotide polymorphism (SNP) markers revealed quantitative trait loci (QTL) on chromosomes 7, 11 and 14. In the advanced intercross line study, the location of the QTL on chromosome 14 was confirmed and refined and two new QTLs were identified located on chromosomes 4 and 16. Pathway and re-sequencing data analysis of the genes located in the QTL candidate regions identified potential pathways, networks and candidate resistance genes. Finally, gene expression analyses were performed for some of the candidate resistance genes to support the results.

Conclusion: Campylobacter resistance in chickens is a complex trait, possibly involving the Major Histocompatibility Complex, innate and adaptive immune responses, cadherins and other factors. Two of the QTLs for Campylobacter resistance are co-located with Salmonella resistance loci, indicating that it may be possible to breed simultaneously for enhanced resistance to both zoonoses.

No MeSH data available.


Related in: MedlinePlus

Pathway analysis using the IPA software. The most highly represented canonical pathways of genes located at the candidate regions for Campylobacter colonisation resistance derived from the back-cross (above) and the advance intercross line (below) experiments. The solid yellow line represents the significance threshold. The line with squares represents the ratio of the genes represented within each pathway to the total number of genes in the pathway
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Fig3: Pathway analysis using the IPA software. The most highly represented canonical pathways of genes located at the candidate regions for Campylobacter colonisation resistance derived from the back-cross (above) and the advance intercross line (below) experiments. The solid yellow line represents the significance threshold. The line with squares represents the ratio of the genes represented within each pathway to the total number of genes in the pathway

Mentions: To identify potential canonical pathways and networks underlying the QTLs detected, we performed pathway analysis using the genes located in these regions. Pathways involved in innate and adaptive immune response, inflammatory response, response to infectious diseases, cell signalling and adhesion, and metabolism constituted the majority of the pathways highlighted for both back-cross and AIL results (Fig. 3). Moreover, two networks of molecular interactions related to immune response were constructed using the list of candidate genes for AIL (Additional file 11: Figure S7).Fig. 3


The genomic architecture of resistance to Campylobacter jejuni intestinal colonisation in chickens.

Psifidi A, Fife M, Howell J, Matika O, van Diemen PM, Kuo R, Smith J, Hocking PM, Salmon N, Jones MA, Hume DA, Banos G, Stevens MP, Kaiser P - BMC Genomics (2016)

Pathway analysis using the IPA software. The most highly represented canonical pathways of genes located at the candidate regions for Campylobacter colonisation resistance derived from the back-cross (above) and the advance intercross line (below) experiments. The solid yellow line represents the significance threshold. The line with squares represents the ratio of the genes represented within each pathway to the total number of genes in the pathway
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4835825&req=5

Fig3: Pathway analysis using the IPA software. The most highly represented canonical pathways of genes located at the candidate regions for Campylobacter colonisation resistance derived from the back-cross (above) and the advance intercross line (below) experiments. The solid yellow line represents the significance threshold. The line with squares represents the ratio of the genes represented within each pathway to the total number of genes in the pathway
Mentions: To identify potential canonical pathways and networks underlying the QTLs detected, we performed pathway analysis using the genes located in these regions. Pathways involved in innate and adaptive immune response, inflammatory response, response to infectious diseases, cell signalling and adhesion, and metabolism constituted the majority of the pathways highlighted for both back-cross and AIL results (Fig. 3). Moreover, two networks of molecular interactions related to immune response were constructed using the list of candidate genes for AIL (Additional file 11: Figure S7).Fig. 3

Bottom Line: The level of colonisation with Campylobacter jejuni following experimental infection was found to be a quantitative trait.Finally, gene expression analyses were performed for some of the candidate resistance genes to support the results.Two of the QTLs for Campylobacter resistance are co-located with Salmonella resistance loci, indicating that it may be possible to breed simultaneously for enhanced resistance to both zoonoses.

View Article: PubMed Central - PubMed

Affiliation: The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK. Androniki.psifidi@roslin.ed.ac.uk.

ABSTRACT

Background: Campylobacter is the leading cause of foodborne diarrhoeal illness in humans and is mostly acquired from consumption or handling of contaminated poultry meat. In the absence of effective licensed vaccines and inhibitors, selection for chickens with increased resistance to Campylobacter could potentially reduce its subsequent entry into the food chain. Campylobacter intestinal colonisation levels are influenced by the host genetics of the chicken. In the present study, two chicken populations were used to investigate the genetic architecture of avian resistance to colonisation: (i) a back-cross of two White Leghorn derived inbred lines [(61 x N) x N] known to differ in resistance to Campylobacter colonisation and (ii) a 9(th) generation advanced intercross (61 x N) line.

Results: The level of colonisation with Campylobacter jejuni following experimental infection was found to be a quantitative trait. A back-cross experiment using 1,243 fully informative single nucleotide polymorphism (SNP) markers revealed quantitative trait loci (QTL) on chromosomes 7, 11 and 14. In the advanced intercross line study, the location of the QTL on chromosome 14 was confirmed and refined and two new QTLs were identified located on chromosomes 4 and 16. Pathway and re-sequencing data analysis of the genes located in the QTL candidate regions identified potential pathways, networks and candidate resistance genes. Finally, gene expression analyses were performed for some of the candidate resistance genes to support the results.

Conclusion: Campylobacter resistance in chickens is a complex trait, possibly involving the Major Histocompatibility Complex, innate and adaptive immune responses, cadherins and other factors. Two of the QTLs for Campylobacter resistance are co-located with Salmonella resistance loci, indicating that it may be possible to breed simultaneously for enhanced resistance to both zoonoses.

No MeSH data available.


Related in: MedlinePlus